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Articles by Sagar K. Gupta in JoVE
JoVE Bioengineering
Обнаружение и изоляция циркулирующих клеток меланомы использованием Фотоакустическая флоуметрии
Мы разработали потока цитометр использованием лазерной индуцированной ультразвук для выявления циркулирующих клеток меланомы, как ранний индикатор метастазов.
Other articles by Sagar K. Gupta on PubMed
Detection of Melanoma Cells in Vitro Using an Optical Detector of Photoacoustic Waves
Circulating tumor cells have been shown to correlate positively with metastatic disease state in patients with advanced cancer. We have demonstrated the ability to detect melanoma cells in a flow system by generating and detecting photoacoustic waves in melanoma cells. This method is similar to flow cytometry, although using photoacoustics rather than fluorescence. Previously, we used piezoelectric films as our acoustic sensors. However, such films have indicated false-positive signals due to unwanted direct interactions between photons from the high laser fluence in the flow system and the film itself. We have adapted an optical detection scheme that obviates the need for piezoelectric films.
