Translate this page to:
Hebrew
In JoVE (2)
- RNA סינתזה ניתוק מן דג הזברה cDNA עובריים עבור ניתוח ביטוי גנים
- Gene Expression גלובל ניתוח באמצעות פלטפורמה דג הזברה microarray oligonucleotide
Other Publications (4)
Automatic Translation
This translation into Hebrew was automatically generated.
English Version | Other Languages
Articles by Samuel M. Peterson in JoVE
JoVE General
RNA סינתזה ניתוק מן דג הזברה cDNA עובריים עבור ניתוח ביטוי גנים
School of Health Sciences, Purdue University
הבידוד של איכות גבוהה, RNA שלם הוא צעד חיוני בפרוטוקולים מעבדה רבות. הנה, אנחנו מדגימים מיצוי RNA מעוברים דג הזברה כולו סינתזה cDNA עבור יישום עוקבת הפרוצדורות השונות כולל ניתוח ביטוי גנים microarray.
JoVE General
Gene Expression גלובל ניתוח באמצעות פלטפורמה דג הזברה microarray oligonucleotide
School of Health Sciences, Purdue University
Microarrays ג'ין הם כלים רבי עוצמה באפיון ביטוי גנים ברמת הגנום כולו. טכנולוגיה זו יישומים במגוון רחב של תחומים לרבות ביולוגי ביולוגיה התפתחותית הרעלים. בסרטון הזה, אנחנו פרט פרוטוקול ניתוח ביטוי גנטי גלובלי באמצעות פלטפורמה מקיפה microarray oligonucleotide על דג הזברה.
Other articles by Samuel M. Peterson on PubMed
Cancer Cytogenetics in the Zebrafish
The zebrafish system has been established as a useful model for the study of carcinogenesis. The cytogenetic characterization of the genome is vital for furthering our understanding of the progression of the disease. Establishing a basic description of the zebrafish chromosomal karyotype and markers for each specific chromosome permitted the first cytogenetic characterization of the reference genome and the genome of cancer models. As the field of cancer cytogenetics is highly dependent on technology, each advance in technique and methodology has resulted in a corresponding wave of discoveries. We have witnessed great improvement in the resolution of the assays allowing for more detailed characterization of cytogenetic abnormalities, including the efficient and accurate identification of DNA copy number alterations of specific chromosomal regions. Herein, we will discuss major advancements in the field of cytogenetics, along with examples of how these technologies have been utilized in studies to characterize zebrafish cancer disease models. Finally, we will discuss the current state of the field and how microarray technology are being implemented to scan the whole genome at high resolution for DNA copy number alterations observed in various cancer types throughout the progression of the disease.
Global Gene Expression Analysis Reveals Dynamic and Developmental Stage-dependent Enrichment of Lead-induced Neurological Gene Alterations
The underlying genetic mechanisms specific to subtle neurological alterations associated with environmental lead (Pb) exposures have not been clearly elucidated.
Decreased Axonal Density and Altered Expression Profiles of Axonal Guidance Genes Underlying Lead (Pb) Neurodevelopmental Toxicity at Early Embryonic Stages in the Zebrafish
Previous studies have reported that environmental lead (Pb) exposure can result in neurological alterations in children leading to reduced IQ, attention deficit hyperactivity disorder, and diminished reading and learning abilities. However, the specific alterations in neurodevelopmental morphology and the underlying genetic mechanisms of these alterations have not yet been thoroughly defined. To investigate alterations in neurologic morphology and test the hypothesis that developmental Pb neurotoxicity is partially mediated through alterations in neuronal growth and transport function of axons, the changes of specific axon tracts in the embryonic zebrafish brain were observed with anti-acetylated α-tubulin staining at several developmental time points through 36hours post fertilization (hpf). In addition, the role of a subset of axonogenesis-related genes including shha, epha4b, netrin1b, netrin2, and noiwas investigated with real-time quantitative PCR (qPCR). Pb treatment resulted in decreased axonal density at 18, 20, and 24hpf for specific axon tracts in the midbrain and forebrain. These observations corresponded to an observed down-regulation of shha and epha4b at 14 and 16hpf, respectively. The axonal density in Pb exposed individuals at later stages (30 and 36hpf) was not significantly different from controls. An overexpression of netrin2 at these two developmental stages suggests a novel role for this gene in regulating axonal density specific to Pb neurotoxicity. Although no significant differences in axonal density was observed in the two later developmental stages, further studies are needed to determine if the morphologic alterations observed at the earlier stages will have lasting functional impacts.
Extensive Genetic Diversity and Substructuring Among Zebrafish Strains Revealed Through Copy Number Variant Analysis
Copy number variants (CNVs) represent a substantial source of genomic variation in vertebrates and have been associated with numerous human diseases. Despite this, the extent of CNVs in the zebrafish, an important model for human disease, remains unknown. Using 80 zebrafish genomes, representing three commonly used laboratory strains and one native population, we constructed a genome-wide, high-resolution CNV map for the zebrafish comprising 6,080 CNV elements and encompassing 14.6% of the zebrafish reference genome. This amount of copy number variation is four times that previously observed in other vertebrates, including humans. Moreover, 69% of the CNV elements exhibited strain specificity, with the highest number observed for Tubingen. This variation likely arose, in part, from Tubingen's large founding size and composite population origin. Additional population genetic studies also provided important insight into the origins and substructure of these commonly used laboratory strains. This extensive variation among and within zebrafish strains may have functional effects that impact phenotype and, if not properly addressed, such extensive levels of germ-line variation and population substructure in this commonly used model organism can potentially confound studies intended for translation to human diseases.
