Articles by Sandrine Paule Claus in JoVE

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by ¹H NMR Spectroscopy

JoVE 3642 12/15/2011

Peter Heath¹, Sandrine Paule Claus²

¹School of Chemistry, Food and Pharmacy, The University of Reading, ²Department of Nutritional Sciences, The University of Reading

A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.