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In JoVE (1)
- Nükleer Manyetik Rezonans Spektroskopisi ile Çevre metabolomik için düşük yoğunluklu Planktonik topluluklardan Metabolitlerinin Konsantrasyon
Other Publications (12)
- Circulation Journal : Official Journal of the Japanese Circulation Society
- The Journal of Bone and Joint Surgery. American Volume
- International Orthopaedics
- Nihon Naika Gakkai Zasshi. The Journal of the Japanese Society of Internal Medicine
- APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
- Plant & Cell Physiology
- Physiologia Plantarum
- Journal of Plant Research
- Nihon Arukōru Yakubutsu Igakkai Zasshi = Japanese Journal of Alcohol Studies & Drug Dependence
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Articles by Seiji Yoshida in JoVE
Nükleer Manyetik Rezonans Spektroskopisi ile Çevre metabolomik için düşük yoğunluklu Planktonik topluluklardan Metabolitlerinin Konsantrasyon
R. Craig Everroad*1, Seiji Yoshida*2, Yuuri Tsuboi1, Yasuhiro Date3, Jun Kikuchi2,3,4, Shigeharu Moriya1,2
1Biosphere Oriented Biology Research Unit, RIKEN Advanced Science Institute, 2Graduate School of Nanobioscience, Yokohama City University, 3Advanced NMR Metabomics Research Team, RIKEN Plant Science Center, 4Graduate School of Bioagricultural Science, Nagoya University
Mikrobiyal planktonik toplulukların metaboliti çıkarımı için bir yöntem sunulmaktadır. Tüm topluluk örnekleme özel hazırlanmış filtreler üzerine filtrasyon sağlanır. Liyofilizasyon sonra, sulu çözünür metabolitleri ekstre edilir. Bu yaklaşım doğal ya da deneysel mikrobiyal toplulukların trans-omik araştırmalar çevre metabolomik uygulaması için izin verir.
Other articles by Seiji Yoshida on PubMed
Circulation Journal : Official Journal of the Japanese Circulation Society. Feb, 2002 | Pubmed ID: 11999648
Experiments were conducted to determine whether the medial amygdala could control hemodynamics through the GABA receptor in anesthetized rats. Bicuculline (10-100pmol), a GABA(A) antagonist, injected into the anterior basomedial amygdala (medial amygdala) produced dose-related increases in blood pressure (BP) and heart rate (HR). Following microinjection of a 100pmol dose, plasma norepinephrine and epinephrine also rose significantly. In a group that had undergone bilateral adrenalectomy, the same dose of bicuculline into the same region of the amygdala caused similar increases in BP and HR to those produced in normal rats. Pretreatment with an intraperitoneal injection of hexamethonium prevented these responses. When bicuculline was given intravenously (100pmol) it failed to effect these cardiovascular changes. Bicuculline-induced hypertension and tachycardia were inhibited by microinjection of muscimol, a GABA agonist, into the anterior basomedial amygdala. The results thus demonstrate that the influence of bicuculline on BP and HR is through action on the anterior basomedial amygdala and direct sympathetic outflow to heart and vascular smooth muscle. There is tonic GABAergic inhibition of sympathetic outflow in the medial amygdala that plays a part in regulating hemodynamics in the limbic system.
The Journal of Bone and Joint Surgery. American Volume. Aug, 2002 | Pubmed ID: 12177277
Orthopedics. Aug, 2002 | Pubmed ID: 12195916
International Orthopaedics. 2003 | Pubmed ID: 12799755
We treated 15 patients with malignant bone and soft-tissue tumours of the shoulder girdle using limb salvage operations involving resection of the proximal humerus, scapula and clavicle. The oncological and functional outcomes were evaluated in all patients after an average of 5 (1-13) years. Oncological evaluation revealed that in 11 patients with adequate surgical margins six were continuously disease free and two had no evidence of disease. Three patients with marginal surgical margins had all died. We suggest that only where adequate surgical margins can be obtained should operations be performed. Forequarter amputation is still indicated in patients with complicated neurovascular involvement. Functional evaluation showed that scapular resections, especially glenoid resections, provided poor results (mean score 54%) in comparison with patients without such resection (mean score 82%).
Nihon Naika Gakkai Zasshi. The Journal of the Japanese Society of Internal Medicine. Jul, 2003 | Pubmed ID: 12924279
APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Jun, 2005 | Pubmed ID: 15996162
A case of collision tumor in the left maxillary sinus composed of adenoid cystic carcinoma (ACC) and metastatic hepatocellular carcinoma (HCC) is reported. Radiographic examination revealed masses in the liver and bilateral lung metastases. Histologically, proliferation of tumor cells with resemblance to HCC was observed, in addition to the ACC. For this reason, differential diagnosis between a second primary tumor and metastasis was made. The metastatic lesion immunohistochemically showed positivity for hepatocyte antigen (OCH1E5) and protein induced by vitamin K absence or antagonist II (PIVKA-II), sustaining the HCC diagnosis. Primary ACC and metastatic HCC in the maxillary sinus are rare, and this may therefore be the first case of maxillary sinus tumor with both these elements.
Plant & Cell Physiology. Feb, 2006 | Pubmed ID: 16361320
Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance.
Immunohistochemical Localisation of Surfactant Proteins A and D, and KL-6 in Pulmonary Alveolar Proteinosis
Pathology. Aug, 2008 | Pubmed ID: 18604747
An Unidentified Ultraviolet-B-specific Photoreceptor Mediates Transcriptional Activation of the Cyclobutane Pyrimidine Dimer Photolyase Gene in Plants
Planta. Dec, 2008 | Pubmed ID: 18825406
Cyclobutane pyrimidine dimers (CPDs) constitute a majority of DNA lesions caused by ultraviolet-B (UVB). CPD photolyase, which rapidly repairs CPDs, is essential for plant survival under sunlight containing UVB. Our earlier results that the transcription of the cucumber CPD photolyase gene (CsPHR) was activated by light have prompted us to propose that this light-driven transcriptional activation would allow plants to meet the need of the photolyase activity upon challenges of UVB from sunlight. However, molecular mechanisms underlying the light-dependent transcriptional activation of CsPHR were unknown. In order to understand spectroscopic aspects of the plant response, we investigated the wavelength-dependence (action spectra) of the light-dependent transcriptional activation of CsPHR. In both cucumber seedlings and transgenic Arabidopsis seedlings expressing reporter genes under the control of the CsPHR promoter, the action spectra exhibited the most predominant peak in the long-wavelength UVB waveband (around 310 nm). In addition, a 95-bp cis-acting region in the CsPHR promoter was identified to be essential for the UVB-driven transcriptional activation of CsPHR. Thus, we concluded that the photoperception of long-wavelength UVB by UVB photoreceptor(s) led to the induction of the CsPHR transcription via a conserved cis-acting element.
Physiologia Plantarum. Jul, 2009 | Pubmed ID: 19453511
Ozone produces reactive oxygen species and induces the synthesis of phytohormones, including ethylene and salicylic acid. These phytohormones act as signal molecules that enhance cell death in response to ozone exposure. However, some studies have shown that ethylene and salicylic acid can instead decrease the magnitude of ozone-induced cell death. Therefore, we studied the defensive roles of ethylene and salicylic acid against ozone. Unlike the wild-type, Col-0, Arabidopsis mutants deficient in ethylene signaling (ein2) or salicylic acid biosynthesis (sid2) generated high levels of superoxide and exhibited visible leaf injury, indicating that ethylene and salicylic acid can reduce ozone damage. Macroarray analysis suggested that the ethylene and salicylic acid defects influenced glutathione (GSH) metabolism. Increases in the reduced form of GSH occurred in Col-0 6 h after ozone exposure, but little GSH was detected in ein2 and sid2 mutants, suggesting that GSH levels were affected by ethylene or salicylic acid signaling. We performed gene expression analysis by real-time polymerase chain reaction using genes involved in GSH metabolism. Induction of gamma-glutamylcysteine synthetase (GSH1), glutathione synthetase (GSH2), and glutathione reductase 1 (GR1) expression occurred normally in Col-0, but at much lower levels in ein2 and sid2. Enzymatic activities of GSH1 and GSH2 in ein2 and sid2 were significantly lower than in Col-0. Moreover, ozone-induced leaf damage observed in ein2 and sid2 was mitigated by artificial elevation of GSH content. Our results suggest that ethylene and salicylic acid protect against ozone-induced leaf injury by increasing de novo biosynthesis of GSH.
Expression and Functions of Myo-inositol Monophosphatase Family Genes in Seed Development of Arabidopsis
Journal of Plant Research. May, 2011 | Pubmed ID: 20960216
Myo-inositol monophosphatase (IMP) catalyzes the dephosphorylation of myo-inositol 3-phosphate in the last step of myo-inositol biosynthesis. IMP is also important in phosphate metabolism and is required for the biosynthesis of cell wall polysaccharides, phytic acid, and phosphatidylinositol. In Arabidopsis, IMP is encoded by VTC4. There are, however, two additional IMP candidate genes, IMPL1 and IMPL2, which have not yet been elucidated. In our genetic studies of Arabidopsis IMP genes, only the loss-of-function mutant impl2 showed embryonic lethality at the globular stage. All IMP genes were expressed in a similar manner both in the vegetative and reproductive organs. In developing seeds, expression of IMP genes was not coupled with the expression of the genes encoding myo-inositol phosphate synthases, which supply the substrate for IMPs in the de novo synthesis pathway. Instead, expression of IMP genes was correlated with expression of the gene for myo-inositol polyphosphate 1-phosphatase (SAL1), which is involved in the myo-inositol salvage pathway, suggesting a possible salvage pathway role in seed development. Moreover, the partial rescue of the impl2 phenotype by histidine application implies that IMPL2 is also involved in histidine biosynthesis during embryo development.
Nihon Arukōru Yakubutsu Igakkai Zasshi = Japanese Journal of Alcohol Studies & Drug Dependence. Dec, 2011 | Pubmed ID: 22413564
Alcoholism is a disease that greatly ruins body mental health, family's health, and a social property. Moreover, it is a disease that a few drinking are connected with the danger of the relapse because there is a control trouble of the drinking desire. The purpose of this survey is to clarify the relation between television commercial of alcoholic beverage (CM) and alcoholism.