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Articles by Shabnam Tavassoli in JoVE
JoVE General
En hög genomströmning metod för att globalt studera organell morfologi i S. cerevisiae
Department of Cellular and Physiological Sciences, University of British Columbia - UBC
GFP-fusionsproteiner används ofta för att visualisera organeller av konfokalmikroskopi. Kräver dock screening för mutationer som påverkar morfologi av organeller i allmänhet enskilda mutagenes och är tidskrävande. Här visar vi en metod att samtidigt införliva organell-GFP markörer i nästan 5000 icke väsentliga gener i jäst.
Other articles by Shabnam Tavassoli on PubMed
Inheritance of Cortical ER in Yeast is Required for Normal Septin Organization
How cells monitor the distribution of organelles is largely unknown. In budding yeast, the largest subdomain of the endoplasmic reticulum (ER) is a network of cortical ER (cER) that adheres to the plasma membrane. Delivery of cER from mother cells to buds, which is termed cER inheritance, occurs as an orderly process early in budding. We find that cER inheritance is defective in cells lacking Scs2, a yeast homologue of the integral ER membrane protein VAP (vesicle-associated membrane protein-associated protein) conserved in all eukaryotes. Scs2 and human VAP both target yeast bud tips, suggesting a conserved action of VAP in attaching ER to sites of polarized growth. In addition, the loss of either Scs2 or Ice2 (another protein involved in cER inheritance) perturbs septin assembly at the bud neck. This perturbation leads to a delay in the transition through G2, activating the Saccharomyces wee1 kinase (Swe1) and the morphogenesis checkpoint. Thus, we identify a mechanism involved in sensing the distribution of ER.
