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In JoVE (1)
Other Publications (5)
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Articles by Somaieh Ahmadian in JoVE
MCF-7 Hücre Line Nanogenomedicine Hücresel Toksisite: MTT assay
Somaieh Ahmadian1,2, Jaleh Barar1,3, Amir Ata Saei1, Mohammad Amin Abolghassemi Fakhree2, Yadollah Omidi1,3
1Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University (Medical Sciences), 2Gifted and Talented Students Office, Educational Development Center, Tabriz University (Medical Sciences), 3School of Advanced Biomedical Sciences, Tabriz University (Medical Sciences)
MTT testi sarı MTT azaltılması ve suda çözünmeyen mor formazan üretimine dayalı hücre canlılığının değerlendirilmesi için kolay ve tekrarlanabilir kolorimetrik assay. Burada, nanogenomedicine tedavi üzerine MCF-7 hücre canlılığı değerlendirildi.
Other articles by Somaieh Ahmadian on PubMed
Characterization and Astrocytic Modulation of System L Transporters in Brain Microvasculature Endothelial Cells
Cell Biochemistry and Function. Apr, 2008 | Pubmed ID: 18210381
Brain trafficking of amino acids is mainly mediated by amino acids transport machineries of the blood-brain barrier (BBB), where astrocytes play a key maintenance role. However, little is known about astrocytes impacts on such transport systems, in particular system L that consists of large and small neutral amino acids (NAAs) transporters, that is, LAT1/4F2hc and LAT2/4F2hc, respectively. In the current investigation, functionality and expression of system L were studied in the immortalized mouse brain microvascular endothelial b.End3 cells cocultured with astrocytes or treated with astrocyte-conditioned media (ACM). LAT2/4F2hc mediated luminal uptake of L-phenylalanine and L-leucine resulted in significantly decreased affinity of system L in b.End3 cells treated with ACM, while LAT2/4F2hc mediated luminal uptake of L-alanine remained unchanged. Gene expression analysis revealed marked upregulation of LAT1 and 4F2hc, but downregulation of LAT2 in b.End3 cells cultured with ACM. The basal to apical transport of L-phenylalanine and L-alanine appeared to be significantly greater than that of the apical to basal direction in b.End3 cells indicating an efflux functionality of system L. No marked influence was observed for transport of L-phenylalanine in b.End3 cells cocultured with astrocytes, while a slight decrease was seen for L-alanine in the basal to apical direction. Based on our findings, we propose that system L functions as influx and/or efflux transport machinery displaying a greater propensity for the outward transport of large and small NAAs. Astrocytes appeared to modulate the transcriptic expression and uptake functionalities of system L, but not the transport activities.
Microarray Analysis of the Toxicogenomics and the Genotoxic Potential of a Cationic Lipid-based Gene Delivery Nanosystem in Human Alveolar Epithelial A549 Cells
Toxicology Mechanisms and Methods. 2008 | Pubmed ID: 20020904
ABSTRACT Viral and nonviral vectors have been widely used in gene therapy as delivery reagents for nucleic acids. Toxicity with viral vectors has increasingly led to the search for suitable nonviral vectors, such as cationic lipids/polymers, as potentially safer alternatives. However, little is known about the genomic toxicity of these delivery systems in target cells/tissues. In the current investigation, we report on the toxicogenomics and genotoxicity of cationic lipid Oligofectamine (OF) nanosystems in human alveolar epithelial A549 cells. To investigate the nature and the ontology of the gene expression changes in A549 cells upon treatment with OF nanoliposomes, microarray gene expression profiling methodology was utilized. For microarray analysis, cyanine (Cy3/Cy5)-labeled cDNA samples from treated and untreated cells were hybridized on target arrays housing 200 genes. Both OF and OF-DNA lipoplex induced significant gene expression changes belonging to the different genomic ontologies such as cell defense and apoptosis pathways. Flow cytometry analyses revealed induction of apoptosis in A549 cells treated with these nanosystems that is likely due to interactions and/or deterioration of the cell membranes. However, no DNA damage was detected by the Comet assay. These data suggest that cationic nanoliposomes in the absence of direct DNA damage elicit multiple gene expression changes in A549 cells that may compromise the main goals of gene medicine where only therapy-defined gene changes are required.
Medical Hypotheses. Mar, 2009 | Pubmed ID: 19013024
Medical Hypotheses. May, 2009 | Pubmed ID: 19171436
Medical Hypotheses. Oct, 2009 | Pubmed ID: 19560876