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In JoVE (2)
- आयरन ऑक्साइड के साथ hESCs और hMSCs लेबल vivo में ट्रैकिंग में गैर इनवेसिव के लिए एमआर इमेजिंग नैनोकणों के साथ
- गैर इनवेसिव पता लगाने के लिए प्रतिदीप्त रंगों के साथ ऑप्टिकल इमेजिंग के साथ स्टेम सेल लेबल
Other Publications (17)
- Academic Radiology
- European Radiology
- AJR. American Journal of Roentgenology
- Pediatric Radiology
- Contrast Media & Molecular Imaging
- Journal of Translational Medicine
- Molecular Imaging
- Pediatric Radiology
- Journal of Translational Medicine
- Optics Express
- Cell Transplantation
- Radiology
- Arthritis and Rheumatism
- Molecular Imaging
- Pediatric Radiology
- Frontiers in Human Neuroscience
- Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
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Articles by Sophie Boddington in JoVE
JoVE General
आयरन ऑक्साइड के साथ hESCs और hMSCs लेबल vivo में ट्रैकिंग में गैर इनवेसिव के लिए एमआर इमेजिंग नैनोकणों के साथ
नई स्टेम सेल के उपचारों के मूल्यांकन के लिए गैर invasively के लिए महत्वपूर्ण है vivo में इंजेक्शन कोशिकाओं को ट्रैक. यह वीडियो तुम्हें दिखाने कैसे vivo में बाद एमआर इमेजिंग के लिए vivo में मानव mesenchymal और लोहे के आक्साइड आधारित विपरीत एजेंटों के साथ भ्रूण स्टेम कोशिकाओं लेबल होगा.
JoVE General
गैर इनवेसिव पता लगाने के लिए प्रतिदीप्त रंगों के साथ ऑप्टिकल इमेजिंग के साथ स्टेम सेल लेबल
इस वीडियो में मानव भ्रूण स्टेम कोशिकाओं और फ्लोरोसेंट रंजक के साथ mesenchymal स्टेम सेल की लेबलिंग के लिए तकनीक से पता चलता है. इस तकनीक vivo में एक ऑप्टिकल इमेजिंग के साथ प्रतिरोपित स्टेम कोशिकाओं की ट्रैकिंग के लिए और प्रतिदीप्ति माइक्रोस्कोपी के साथ histopathological सहसंबंध के लिए इस्तेमाल किया जा सकता है.
Other articles by Sophie Boddington on PubMed
Postoperative Evaluation of Complex Aortovisceral and Aortorenal Reconstructions by Magnetic Resonance Angiography
To assess the ability of magnetic resonance angiography (MRA) to evaluate complex vascular bypass reconstructions of the abdominal aorta and its major branches in the postoperative period.
Cell Labeling with the Positive MR Contrast Agent Gadofluorine M
The purpose of this study was to label human monocytes with Gadofluorine M by simple incubation for subsequent cell depiction at 1.5 and 3 T. Gadofluorine M displays a high r(1) relaxivity and is spontaneously phagocytosed by macrophages. Human monocytes were incubated with Gadofluorine M-Cy at varying concentrations and incubation times and underwent MR imaging at 1.5 and 3 T at increasing time intervals after the labeling procedure. R1-relaxation rates and r1 relaxivities of the labeled cells and non-labeled controls were determined. Cellular contrast agent uptake was examined by fluorescence microscopy and quantified by ICP-AES. Efficient cell labeling was achieved after incubation of the cells with 25 mM Gd Gadofluorine M for 12 h, resulting in a maximal uptake of 0.3 fmol Gd/cell without impairment of cell viability. Fluorescence microscopy confirmed internalization of the fluorescent contrast agent by monocytes. The r1 relaxivity of the labeled cells was 137 mM(-1)s(-1) at 1.5 T and 80.46 mM(-1)s(-1) at 3 T. Imaging studies showed stable labeling for at least 7 days. Human monocytes can be effectively labeled for MR imaging with Gadofluorine M. Potential in vivo cell-tracking applications include targeting of inflammatory processes with Gadofluorine-labeled leukocytes or monitoring of stem cell therapies for the treatment of arthritis.
Anterior Layering of Excreted 18F-FDG in the Bladder on PET/CT: Frequency and Cause
OBJECTIVE: The objective of our study was to determine the frequency and cause of anterior layering of excreted 18F-FDG in the bladder on PET/CT. CONCLUSION: Anterior layering of excreted FDG in the bladder is commonly seen on PET/CT scans obtained with i.v. iodinated contrast material and is due to displacement of FDG by excreted iodinated contrast material; this phenomenon may unmask FDG-avid bladder disease.
MR Imaging of Ovarian Tumors Using Folate-receptor-targeted Contrast Agents
Because of its over-expression in many human tumors, the folate receptor (FR) is a promising target for tumor-specific imaging.
Improved Fluorescence of Indocyanine Green in Vitro and in Vivo After Simple Cooling Procedures
Indocyanine green (ICG) is a contrast agent used for detecting angiogenesis with optical imaging (OI). The purpose of this study was to investigate whether cooling procedures increase the signal yield of ICG with OI. Test samples of 0.05 and 0.02 mM ICG in 40% DMSO and 60% DMEM underwent OI at four different temperatures (5, 37, 55 and 75 degrees C). In addition, six athymic rats with an antigen-induced arthritis of the knee and ankle joints underwent OI before and after injection of ICG (10 mg/ml, dose 15 mg/kg) on two separate days with and without cooling of the joints. The fluorescent signals of the test samples and arthritic joints were measured and evaluated for significant differences before and after cooling with a t-test. In vitro studies showed a strong negative correlation between ICG temperature and fluorescent signal. The mean fluorescent signal of arthritic joints (measured in efficiency) was 0.345 before ICG-injection, 4.55 after ICG-injection and before cooling and 9.71 after ICG-injection and after cooling. The fluorescent signal enhancement of arthritic joints with ICG-enhanced OI images increased significantly after cooling (p = 0.02). The signal yield of ICG can be significantly increased by cooling the target pathology. The primary underlying cause of the temperature dependence of ICG is enhanced collisional quenching with increasing temperature. This simple cooling method may be immediately helpful to increase the fluorescence signal yield in current ICG-enhanced OI-studies in patients.
Detection of Postoperative Granulation Tissue with an ICG-enhanced Integrated OI-/X-ray System
The development of postoperative granulation tissue is one of the main postoperative risks after lumbar spine surgery. This granulation tissue may lead to persistent or new clinical symptoms or complicate a follow up surgery. A sensitive non-invasive imaging technique, that could diagnose this granulation tissue at the bedside, would help to develop appropriate treatments. Thus, the purpose of this study was to establish a fast and economic imaging tool for the diagnosis of granulation tissue after lumbar spine surgery, using a new integrated Optical Imaging (OI)/X-ray imaging system and the FDA-approved fluorescent contrast agent Indocyanine Green (ICG).
Optical Imaging of Cellular Immunotherapy Against Prostate Cancer
The purpose of this study was to track fluorophore-labeled, tumor-targeted natural killer (NK) cells to human prostate cancer xenografts with optical imaging (OI). NK-92-scFv(MOC31)-zeta cells targeted to the epithelial cell adhesion molecule (EpCAM) antigen on prostate cancer cells and nontargeted NK-92 parental cells were labeled with the near-infrared dye DiD (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine). The fluorescence, viability, and cytotoxicity of the labeled cells were evaluated. Subsequently, 12 athymic rats with prostate cancer xenografts underwent OI scans before and up to 24 hours postinjection of DiD-labeled parental NK-92 cells or NK-92-scFv(MOC31)-zeta cells. The tumor fluorescence intensity was measured and compared between pre- and postinjection scans and between both groups using t-tests. OI data were confirmed with fluorescence microscopy. In vitro studies demonstrated a significant increase in the fluorescence of labeled cells compared with unlabeled controls, which persisted over a period of 24 hours without any significant change in the viability. In vivo studies demonstrated a significant increase in tumor fluorescence at 24 hours postinjection of tumor-targeted NK-92-scFv(MOC31)-zeta cells but not parental NK cells. Ex vivo OI scans and fluorescence microscopy confirmed a specific accumulation of NK-92-scFv(MOC31)-zeta cells but not parental NK cells in the tumors. Tumor-targeted NK-92-scFv(MOC31)-zeta cells could be tracked to prostate cancer xenografts with OI.
Decreased Aortic Growth and Middle Aortic Syndrome in Patients with Neuroblastoma After Radiation Therapy
Long-term CT follow-up studies are required in pediatric patients who have received intraoperative radiation therapy (IORT) and external beam radiation therapy (EBRT) to assess vascular toxicities and to determine the exact complication rate.
Optical Imaging of the Peri-tumoral Inflammatory Response in Breast Cancer
Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging.
An Optical Imaging Method to Monitor Stem Cell Migration in a Model of Immune-mediated Arthritis
The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
Labeling Human Embryonic Stem Cell-derived Cardiomyocytes with Indocyanine Green for Noninvasive Tracking with Optical Imaging: an FDA-compatible Alternative to Firefly Luciferase
Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) have demonstrated the ability to improve myocardial function following transplantation into an ischemic heart; however, the functional benefits are transient possibly due to poor cell retention. A diagnostic technique that could visualize transplanted hESC-CMs could help to optimize stem cell delivery techniques. Thus, the purpose of this study was to develop a labeling technique for hESCs and hESC-CMs with the FDA-approved contrast agent indocyanine green (ICG) for optical imaging (OI). hESCs were labeled with 0.5, 1.0, 2.0, and 2.5 mg/ml of ICG for 30, 45, and 60 min at 37 degrees C. Longitudinal OI studies were performed with both hESCs and hESC-CMs. The expression of surface proteins was assessed with immunofluorescent staining. hESCs labeled with 2 mg ICG/ml for 60 min achieved maximum fluorescence. Longitudinal studies revealed that the fluorescent signal was equivalent to controls at 120 h postlabeling. The fluorescence signal of hESCs and hESC-CMs at 1, 24, and 48 h was significantly higher compared to precontrast data (p < 0.05). Immunocytochemistry revealed retention of cell-specific surface and nuclear markers postlabeling. These data demonstrate that hESCs and hESC-CMs labeled with ICG show a significant fluorescence up to 48 h and can be visualized with OI. The labeling procedure does not impair the viability or functional integrity of the cells. The technique may be useful for assessing different delivery routes in order to improve the engraftment of transplanted hESC-CMs or other stem cell progenitors.
Breast Cancers: MR Imaging of Folate-receptor Expression with the Folate-specific Nanoparticle P1133
To assess the capability of the folate receptor (FR)-targeted ultrasmall superparamagnetic iron oxide (USPIO) P1133 to provide FR-specific enhancement of breast cancers on magnetic resonance (MR) images.
Indocyanine Green-enhanced Imaging of Antigen-induced Arthritis with an Integrated Optical Imaging/radiography System
To evaluate a combined indocyanine green-enhanced optical imaging/radiography system for the detection of arthritic joints in a rat model of antigen-induced arthritis.
In Vivo Magnetic Resonance Imaging and Optical Imaging Comparison of Viable and Nonviable Mesenchymal Stem Cells with a Bifunctional Label
The purpose of this study was to compare viable and nonviable bilabeled mesenchymal stem cells (MSCs) in arthritic joints with magnetic resonance imaging (MRI) and optical imaging (OI). MSCs were labeled with ferucarbotran and DiD. MRI and OI of bilabeled cells were compared with controls. Six rats with arthritis received intra-articular injections of bilabeled viable MSCs into the right knee and nonviable MSCs into the left knee. Animals underwent MRI and OI preinjection and at 4, 24, 48, and 72 hours postinjection. The results were analyzed with a mixed random effects model and Fisher probability. Bilabeled MSCs showed increased MRI and OI signals compared to unlabeled controls (p < .0001). After intra-articular injection, bilabeled MSCs caused significant T2 and T2* effect on MRI and fluorescence on OI up to 72 hours postinjection (p < .05). There was no significant difference between viable and nonviable MSC signal in the knee joints; however, some of the viable cells migrated to an adjacent inflamed ankle joint (p < .05). Immunohistochemistry confirmed viable MSCs in right knee and ankle joints and nonviable MSCs in the left knee. Viable and nonviable cells could not be differentiated with MRI or OI signal intensity but were differentiated based on their ability to migrate in vivo.
Labeling Human Embryonic Stem-cell-derived Cardiomyocytes for Tracking with MR Imaging
Human embryonic stem cells (hESC) can generate cardiomyocytes (CM), which offer promising treatments for cardiomyopathies in children. However, challenges for clinical translation result from loss of transplanted cell from target sites and high cell death. An imaging technique that noninvasively and repetitively monitors transplanted hESC-CM could guide improvements in transplantation techniques and advance therapies.
The N250 Brain Potential to Personally Familiar and Newly Learned Faces and Objects
Studies employing event-related potentials have shown that when participants are monitoring for a novel target face, the presentation of their own face elicits an enhanced negative brain potential in posterior channels approximately 250 ms after stimulus onset. Here, we investigate whether the own face N250 effect generalizes to other highly familiar objects, specifically, images of the participant's own dog and own car. In our experiments, participants were asked to monitor for a pre-experimentally unfamiliar target face (Joe), a target dog (Experiment 1: Joe's Dog) or a target car (Experiment 2: Joe's Car). The target face and object stimuli were presented with non-target foils that included novel face and object stimuli, the participant's own face, their own dog (Experiment 1), and their own car (Experiment 2). The consistent findings across the two experiments were the following: (1) the N250 potential differentiated the target faces and objects from the non-target face and object foils and (2) despite being non-targets, the own face and own objects produced an N250 response that was equal in magnitude to the target faces and objects by the end of the experiment. Thus, as indicated by its response to personally familiar and recently familiarized faces and objects, the N250 component is a sensitive index of individuated representations in visual memory.
Labeling Human Mesenchymal Stem Cells with Fluorescent Contrast Agents: the Biological Impact
This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis.
