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In JoVE (1)
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Articles by Stephanie Bourin in JoVE
JoVE General
Rotulagem seletiva de proteínas de superfície celular usando Dyes CyDye DIGE Fluor Minimal
Research and Development, GE Healthcare Bio-Sciences AB
Um método simples e específica foi demonstrada para marcação fluorescente e detecção aprimorada de proteínas de superfície das células sem uma etapa de fracionamento. Abundância diferencial de proteínas de superfície celular foi analisada através de eletroforese bidimensional (2-D) e Ettan ™ tecnologia DIGE.
Other articles by Stephanie Bourin on PubMed
A Proteomics Approach to the Study of Absorption, Distribution, Metabolism, Excretion, and Toxicity
A proteomics approach was used to identify liver proteins that displayed altered levels in mice following treatment with a candidate drug. Samples from livers of mice treated with candidate drug or untreated were prepared, quantified, labeled with CyDye DIGE Fluors, and subjected to two-dimensional electrophoresis. Following scanning and imaging of gels from three different isoelectric focusing intervals (3-10, 7-11, 6.2-7.5), automated spot handling was performed on a large number of gel spots including those found to differ more than 20% between the treated and untreated condition. Subsequently, differentially regulated proteins were subjected to a three-step approach of mass spectrometry using (a) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide mass fingerprinting, (b) post-source decay utilizing chemically assisted fragmentation, and (c) liquid chromatography-tandem mass spectrometry. Using this approach we have so far resolved 121 differentially regulated proteins following treatment of mice with the candidate drug and identified 110 of these using mass spectrometry. Such data can potentially give improved molecular insight into the metabolism of drugs as well as the proteins involved in potential toxicity following the treatment. The differentially regulated proteins could be used as targets for metabolic studies or as markers for toxicity.
A Quantitative Proteomic Analysis of Soluble Bronchoalveolar Fluid Proteins from Patients with Sarcoidosis and Chronic Beryllium Disease
Sarcoidosis and chronic beryllium disease (CBD) are granulomatous disorders which can lead to development of chronic inflammation and fibrosis. These diseases have several similarities from their clinical aspects. The aim of this study was to compare the protein profile at the site of active inflammation i.e. the lungs of patients with sarcoidosis and CBD.
