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Articles by Stylianos P. Scordilis in JoVE
JoVE Clinical and Translational Medicine
Dimorfismo Sexo músculo esquelético de Proteomics
1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College
Um conjunto simples e direta de métodos para isolar e determinar a identidade das proteínas mais abundantes expressa no músculo esquelético. Cerca de 800 spots são discernidos em um gel de duas dimensões de 10 mg do músculo, o que permite a determinação de gênero específico expressão da proteína. Esses métodos produzem resultados equivalentes na maioria dos tecidos.
Other articles by Stylianos P. Scordilis on PubMed
Serum Creatine Kinase Activity Varies with Ovulatory Status in Regularly Exercising, Premenopausal Women
The clinical complications associated with an unopposed estrogen environment and luteal phase defects observed in exercising women prompted the examination of the relationship of exercise and endogenous ovarian steroids with serum creatine kinase (CK) activity.
Gender Dimorphism in the Exercise-naïve Murine Skeletal Muscle Proteome
Skeletal muscle is a plastic tissue with known gender dimorphism, especially at the metabolic level. A proteomic comparison of male and female murine biceps brachii was undertaken, resolving an average of 600 protein spots of MW 15-150 kDa and pI 5-8. Twenty-six unique full-length proteins spanning 11 KOG groups demonstrated statistically significant (p<0.05) abundance differences between genders; the majority of these proteins have metabolic functions. Identified glycolytic enzymes demonstrated decreased abundance in females, while abundance differences in identified oxidative phosphorylation enzymes were specific to the proteins rather than to the functional group as a whole. Certain cytoskeletal and stress proteins showed specific expression differences, and all three phosphorylation states of creatine kinase showed significant decreased abundance in females. Expression differences were significant but many were subtle (< or = 2-fold), and known hormonally-regulated proteins were not identified. We conclude that while gender dimorphism is present in non-exercised murine skeletal muscle, the proteome comparison of male and female biceps brachii in exercise-naive mice indicates subtle differences rather than a large or obviously hormonal dimorphism.
