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Articles by Su Fen Ang in JoVE

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प्लास्मिड के Microinjection द्वारा Polarized उपकला कोशिकाओं में छोटे GTPases के फंक्शन का विश्लेषण


JoVE 2645 5/31/2011

Department of Cell and Molecular Biology, Northwestern University

इस अनुच्छेद के विवरण और polarized microinjection तकनीक का उपयोग करके उपकला कोशिकाओं में छोटे GTPases की overexpression विश्लेषण में शामिल प्रक्रियाओं.

Other articles by Su Fen Ang on PubMed

DAAM1 is a Formin Required for Centrosome Re-orientation During Cell Migration

Disheveled-associated activator of morphogenesis 1 (DAAM1) is a formin acting downstream of Wnt signaling that is important for planar cell polarity. It has been shown to promote proper cell polarization during embryonic development in both Xenopus and Drosophila. Importantly, DAAM1 binds to Disheveled (Dvl) and thus functions downstream of the Frizzled receptors. Little is known of how DAAM1 is localized and functions in mammalian cells. We investigate here how DAAM1 affects migration and polarization of cultured cells and conclude that it plays a key role in centrosome polarity.

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