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In JoVE (2)
- Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
- Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Other Publications (25)
- Physical Review Letters
- Radiation Protection Dosimetry
- Nature
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Asian Pacific Journal of Cancer Prevention : APJCP
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Clinical Imaging
- Molecular Biology of the Cell
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
- Physical Review Letters
Articles by Sujata S. Jha in JoVE
JoVE General
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
A technique to identify translational pause sites on mRNA is described. This procedure is based on isolation of nascent polypeptides accumulating on ribosomes during in vitro translation of a target mRNA, followed by the size analysis of the nascent chains using a denaturing gel electrophoresis.
JoVE General
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.
Other articles by Sujata S. Jha on PubMed
Search for New Physics in High P_{T} Like-Sign Dilepton Events at CDF II
We present a search for new physics in events with two high p_{T} leptons of the same electric charge, using data with an integrated luminosity of 6.1  fb^{-1}. The observed data are consistent with standard model predictions. We set 95% C.L. lower limits on the mass of doubly charged scalars decaying to like-sign dileptons, m_{H^{±±}}>190-245  GeV/c^{2}, assuming 100% BR to ee, μμ or eμ.
AN ASSESSMENT OF THE RADIOLOGICAL SCENARIO AROUND URANIUM MINES IN SINGHBHUM EAST DISTRICT, JHARKHAND, INDIA
The present work deals with the prevalent radiological scenario around uranium-mining sites in the Singhbhum East district of Jharkhand state, India. The concentration of naturally occurring radioactive materials (NORMs) was estimated from 27 soil samples collected around three regions in the study area, namely Bagjata, Turamdih and Jaduguda. The mean activity concentrations of (238)U in Bagjata, Turamdih and Jaduguda regions were found to be 128.6, 95.7 and 49.2 Bq kg(-1), respectively. Similarly for (232)Th and (40)K the activity concentrations were found to be 57.3, 78.4, 68.9 and 530, 425 and 615 Bq kg(-1) in the Bagjata, Turamdih and Jaduguda regions, respectively, which are comparable with other reported values worldwide, except for some high values. The calculated gamma dose rate, obtained from the concentrations of (238)U, (232)Th and (40)K in the samples, was compared with the observed dose rate in air. A good correlation (0.96) was observed between the calculated and the observed gamma dose rate. The annual outdoor effective dose rate was estimated and the values falls between 0.04-0.3, 0.07-0.3 and 0.07-.14 mSv y(-1) with mean values of 0.14, 0.12 and 0.11 mSv y(-1) for the Bagjata, Turamdih and Jaduguda regions, respectively. The terrestrial dose rates in all the three regions are comparable with other reported values worldwide, except for a few high values in Greece, Rio Grande Do Norte (Brazil) and Kalpakkam (India).
Exclusion of a Luminous Red Giant As a Companion Star to the Progenitor of Supernova SN 2011fe
Type Ia supernovae are thought to result from a thermonuclear explosion of an accreting white dwarf in a binary system, but little is known of the precise nature of the companion star and the physical properties of the progenitor system. There are two classes of models: double-degenerate (involving two white dwarfs in a close binary system) and single-degenerate models. In the latter, the primary white dwarf accretes material from a secondary companion until conditions are such that carbon ignites, at a mass of 1.38 times the mass of the Sun. The type Ia supernova SN 2011fe was recently detected in a nearby galaxy. Here we report an analysis of archival images of the location of SN 2011fe. The luminosity of the progenitor system (especially the companion star) is 10-100 times fainter than previous limits on other type Ia supernova progenitor systems, allowing us to rule out luminous red giants and almost all helium stars as the mass-donating companion to the exploding white dwarf.
Search for B(s)(0) †’ μ+ μ- and B(0) †’ μ+ μ- Decays with CDF II
A search has been performed for B(s)(0) → μ+ μ- and B(0) → μ+ μ- decays using 7 fb(-1) of integrated luminosity collected by the CDF II detector at the Fermilab Tevatron collider. The observed number of B(0) candidates is consistent with background-only expectations and yields an upper limit on the branching fraction of B(B(0) → μ+ μ-) < 6.0 × 10(-9) at 95% confidence level. We observe an excess of B(s)(0) candidates. The probability that the background processes alone could produce such an excess or larger is 0.27%. The probability that the combination of background and the expected standard model rate of B(s)(0) → μ+ μ- could produce such an excess or larger is 1.9%. These data are used to determine B(B(s)(0)→ μ+ μ-) = (1.8(-0.9) (+1.1)) × 10(-8) and provide an upper limit of B(B(s)(0) → μ+ μ-) < 4.0 × 10(-8) at 95% confidence level.
Search for New T' Particles in Final States with Large Jet Multiplicities and Missing Transverse Energy in P P Collisions at Sqrt[s] = 1.96 TeV
We present a search for a new particle T' decaying to a top quark via T' → t + X, where X goes undetected. We use a data sample corresponding to 5.7 fb(-1) of integrated luminosity of p p collisions with sqrt[s] = 1.96 TeV, collected at Fermilab Tevatron by the CDF II detector. Our search for pair production of T' is focused on the hadronic decay channel, pp → T'T' → tt + XX → bqq b qq + XX. We interpret our results in terms of a model where T' is an exotic fourth generation quark and X is a dark matter particle. The data are consistent with standard model expectations. We set a limit on the generic production of T'T' → tt + XX, excluding the fourth generation exotic quarks T' at 95% confidence level up to m(T') = 400 GeV/c(2) for m(X) ≤ 70 GeV/c(2).
Observation of the Baryonic Flavor-Changing Neutral Current Decay Λ_{b}^{0}→Λμ^{+}μ^{-}
We report the first observation of the baryonic flavor-changing neutral current decay Λ_{b}^{0}→Λμ^{+}μ^{-} with 24 signal events and a statistical significance of 5.8 Gaussian standard deviations. This measurement uses a pp[over ¯] collisions data sample corresponding to 6.8  fb^{-1} at sqrt[s]=1.96  TeV collected by the CDF II detector at the Tevatron collider. The total and differential branching ratios for Λ_{b}^{0}→Λμ^{+}μ^{-} are measured. We find B(Λ_{b}^{0}→Λμ^{+}μ^{-})=[1.73±0.42(stat)±(syst)]×10^{-6}. We also report the first measurement of the differential branching ratio of B_{s}^{0}→ϕμ^{+}μ^{-}, using 49 signal events. In addition, we report branching ratios for B^{+}→K^{+}μ^{+}μ^{-}, B^{0}→K^{0}μ^{+}μ^{-}, and B→K^{*}(892)μ^{+}μ^{-} decays.
Search for a Standard Model Higgs Boson in the H→ZZ→ℓ(+)ℓ(-)νν Decay Channel with the ATLAS Detector
A search for a heavy standard model Higgs boson decaying via H→ZZ→→ℓ(+)ℓ(-)νν, where ℓ=e, μ, is presented. It is based on proton-proton collision data at √s=7 TeV, collected by the ATLAS experiment at the LHC in the first half of 2011 and corresponding to an integrated luminosity of 1.04 fb(-1). The data are compared to the expected standard model backgrounds. The data and the background expectations are found to be in agreement and upper limits are placed on the Higgs boson production cross section over the entire mass window considered; in particular, the production of a standard model Higgs boson is excluded in the region 340
Search for the Higgs Boson in the H→WW→lνjj Decay Channel in Pp Collisions at ˆšs=7 TeV with the ATLAS Detector
A search for a Higgs boson has been performed in the H→WW→ℓνjj channel in 1.04 fb(-1) of pp collision data at √s=7 TeV recorded with the ATLAS detector at the Large Hadron Collider. No significant excess of events is observed over the expected background and limits on the Higgs boson production cross section are derived for a Higgs boson mass in the range 240 GeV
Top-quark Mass Measurement Using Events with Missing Transverse Energy and Jets at CDF
We present a measurement of the top-quark mass using a sample of t ̄t events in 5.7 fb(-1) of integrated luminosity from p ̄p collisions at the Fermilab Tevatron with √s=1.96 TeV and collected by the CDF II Detector. We select events having large missing transverse energy, and four, five, or six jets with at least one jet tagged as coming from a b quark, and reject events with identified charged leptons. This analysis considers events from the semileptonic t ̄t decay channel, including events that contain tau leptons. The measurement is based on a multidimensional template method. We fit the data to signal templates of varying top-quark masses and background templates, and measure a top-quark mass of M(top)=172.32±2.4(stat)±1.0(syst)  GeV/c(2).
Search for a Heavy Toplike Quark in Pp Collisions at ˆšs=1.96 TeV
We present the results of a search for pair production of a heavy toplike (t') quark decaying to Wq final states using data corresponding to an integrated luminosity of 5.6 fb(-1) collected by the CDF II detector in pp collisions at √s=1.96 TeV. We perform parallel searches for t'→Wb and t'→Wq (where q is a generic down-type quark) in events containing a lepton and four or more jets. By performing a fit to the two-dimensional distribution of total transverse energy versus reconstructed t' quark mass, we set upper limits on the t't' production cross section and exclude a standard model fourth-generation t' quark decaying to Wb (Wq) with mass below 358 (340) GeV/c(2) at 95% C.L.
Measurement of Polarization and Search for CP Violation in B(s)0→φφ Decays
We present the first measurement of polarization and CP-violating asymmetries in a B(s)(0) decay into two light vector mesons, B(s)(0)→φφ, and an improved determination of its branching ratio using 295 decays reconstructed in a data sample corresponding to 2.9 fb(-1) of integrated luminosity collected by the CDF experiment at the Fermilab Tevatron collider. The fraction of longitudinal polarization is determined to be f(L)=0.348±0.041(stat)±0.021(syst), and the branching ratio B(B(s)(0)→φφ)=[2.32±0.18(stat)±0.82(syst)]×10(-5). Asymmetries of decay angle distributions sensitive to CP violation are measured to be A(u)=-0.007±0.064(stat)±0.018(syst) and A(v)=-0.120±0.064(stat)±0.016(syst).
Measurement of the B(s)0 Lifetime in Fully and Partially Reconstructed B(s)0→D(s)(-)(Ï•Ï€(-))X Decays in P¯p Collisions at ˆšs=1.96 TeV
We present a measurement of the B(s)(0) lifetime in fully and partially reconstructed B(s)(0)→D(s)(-)(ϕπ(-))X decays in 1.3  fb(-1) collected in pp ¯ collisions at √s=1.96 TeV by the CDF II detector at the Fermilab Tevatron. We measure τ(B(s)(0))=1.518±0.041(stat)±0.027(syst)  ps. The ratio of this result and the world average B(0) lifetime yields τ(B(s)(0))/τ(B(0))=0.99±0.03, which is in agreement with recent theoretical predictions.
Search for Dilepton Resonances in Pp Collisions at ˆšs=7 TeV with the ATLAS Detector
This Letter reports on a search for narrow high-mass resonances decaying into dilepton final states. The data were recorded by the ATLAS experiment in pp collisions at √s=7  TeV at the Large Hadron Collider and correspond to a total integrated luminosity of 1.08 (1.21)  fb(-1) in the e(+)e(-) (μ(+)μ(-)) channel. No statistically significant excess above the standard model expectation is observed and upper limits are set at the 95% C.L. on the cross section times branching fraction of Z' resonances and Randall-Sundrum gravitons decaying into dileptons as a function of the resonance mass. A lower mass limit of 1.83 TeV on the sequential standard model Z' boson is set. A Randall-Sundrum graviton with coupling k/M(Pl)=0.1 is excluded at 95% C.L. for masses below 1.63 TeV.
Neoadjuvant and Adjuvant Therapy with Imatinib for Locally Advanced Gastrointestinal Stromal Tumors in Eastern Indian Patients
Background: Imatinib mesylate is able to at least modify the course of gastrointestinal stromal tumours (GISTs). Neoadjuvant use for locally advanced lesions is evolving as a new treatment paradigm in this hitherto universally fatal disease. Methods and Results: The study patients with locally advanced GIST received neoadjuvant and adjuvant imatinib mesylate. Response was noted as per the RECIST protocol and overall progression free survival was reported. Of 19 patients (mean age 38.5 years, range 26 yrs to 64 yrs) studied, 13 achieved partial response (PR) and 6 a stationary disease (SD) on preoperative imatinib. Histopathological evaluation and grading of responses revealed only moderate and low grade pathological response after imatinib. R0 resection was possible in 13/19 and R1 in 6/19. Imatinib was well tolerated and adverse reactions were minimal. Post operative complications of surgery were not out of the ordinary for a surgical series featuring extensive abdominal surgery. Conclusion: Preoperative imatinib in locally advanced GIST seems to be a reasonable option for locally advanced GIST patients and enough downstaging to allow a resection with microscopically negative margins can be expected in a fairly good proportion of patients.
Search for Scalar Bottom Quark Pair Production with the ATLAS Detector in Pp Collisions at Sqrt[s]=7  TeV
The results of a search for pair production of the scalar partners of bottom quarks in 2.05  fb(-1) of pp collisions at sqrt[s]=7  TeV using the ATLAS experiment are reported. Scalar bottom quarks are searched for in events with large missing transverse momentum and two jets in the final state, where both jets are identified as originating from a bottom quark. In an R-parity conserving minimal supersymmetric scenario, assuming that the scalar bottom quark decays exclusively into a bottom quark and a neutralino, 95% confidence-level upper limits are obtained in the b(1) - χ(1)(0) mass plane such that for neutralino masses below 60 GeV scalar bottom masses up to 390 GeV are excluded.
Search for a Low-mass Standard Model Higgs Boson in the ττ Decay Channel in Pp Collisions at Sqrt[s]=1.96  TeV
We report on a search for the standard model Higgs boson decaying into pairs of τ leptons in pp collisions produced by the Tevatron at sqrt[s]=1.96  TeV. The analyzed data sample was recorded by the CDFII detector and corresponds to an integrated luminosity of 6.0  fb(-1). The search is performed in the final state with one τ decaying leptonically and the second one identified through its semihadronic decay. Since no significant excess is observed, a 95% credibility level upper limit on the production cross section times branching ratio to the ττ final state is set for hypothetical Higgs boson masses between 100 and 150  GeV/c2. For a Higgs boson of 120  GeV/c2 the observed (expected) limit is 14.6 (15.3) the predicted value.
Search for a Higgs Boson in the Diphoton Final State in Pp Collisions at Sqrt[s]=1.96  TeV
A search for a narrow Higgs boson resonance in the diphoton mass spectrum is presented based on data corresponding to 7.0  fb{-1} of integrated luminosity from pp collisions at sqrt[s]=1.96  TeV collected by the CDF experiment. No evidence of such a resonance is observed, and upper limits are set on the cross section times branching ratio of the resonant state as a function of Higgs boson mass. The limits are interpreted in the context of the standard model and one fermiophobic benchmark model where the data exclude fermiophobic Higgs bosons with masses below 114  GeV/c{2} at a 95% Bayesian credibility level.
Abdominal Complications of Chemotherapy: Findings at Computed Tomography
Modern chemotherapy may result in an array of complications that can produce computed tomography (CT) findings in the hepatobiliary, gastrointestinal and urinary systems. This article describes the CT findings that may be seen in abdominal complications of contemporary chemotherapy. Knowledge of the varying CT appearances that can be encountered may facilitate both diagnosis and management in such cases.
The Apoptotic Engulfment Protein Ced-6 Participates in Clathrin-mediated Yolk Uptake in Drosophila Egg Chambers
Clathrin-mediated endocytosis and phagocytosis are both selective surface internalization processes but have little known mechanistic similarity or interdependence. Here we show that the phosphotyrosine-binding (PTB) domain protein Ced-6, a well-established phagocytosis component that operates as a transducer of so-called "eat-me" signals during engulfment of apoptotic cells and microorganisms, is expressed in the female Drosophila germline and that Ced-6 expression correlates with ovarian follicle development. Ced-6 exhibits all the known biochemical properties of a clathrin-associated sorting protein, yet ced-6-null flies are semifertile despite massive accumulation of soluble yolk precursors in the hemolymph. This is because redundant sorting signals within the cytosolic domain of the Drosophila vitellogenin receptor Yolkless, a low density lipoprotein receptor superfamily member, occur; a functional atypical dileucine signal binds to the endocytic AP-2 clathrin adaptor directly. Nonetheless, the Ced-6 PTB domain specifically recognizes the noncanonical Yolkless FXNPXA sorting sequence and in HeLa cells promotes the rapid, clathrin-dependent uptake of a Yolkless chimera lacking the distal dileucine signal. Ced-6 thus operates in vivo as a clathrin adaptor. Because the human Ced-6 orthologue GULP similarly binds to clathrin machinery, localizes to cell surface clathrin-coated structures, and is enriched in placental clathrin-coated vesicles, new possibilities for Ced-6/Gulp operation during phagocytosis must be considered.
Measurement of the ZZ Production Cross Section and Limits on Anomalous Neutral Triple Gauge Couplings in Proton-proton Collisions at Sqrt[s] = 7 TeV with the ATLAS Detector
A measurement of the ZZ production cross section in proton-proton collisions at sqrt[s] = 7 TeV using data corresponding to an integrated luminosity of 1.02 fb(-1) recorded by the ATLAS experiment at the LHC is presented. Twelve events containing two Z boson candidates decaying to electrons and/or muons are observed, with an expected background of 0.3 ± 0.3(stat)(-0.3)(+0.4)(syst) events. The cross section measured in a phase-space region with good detector acceptance and for dilepton masses within the range 66 to 116 GeV is σ(ZZ → ℓ+ ℓ- ℓ+ ℓ-)(fid) = 19.4(-5.2)(+6.3)(stat)(-0.7)(+0.9)(syst) ± 0.7(lumi) fb. The resulting total cross section for on-shell ZZ production, σ(ZZ)(tot) = 8.5(-2.3)(+2.7)(stat)(-0.3)(+0.4)(syst) ± 0.3(lumi) pb, is consistent with the standard model expectation of 6.5(-0.2)(+0.3) pb calculated at the next-to-leading order in QCD. Limits on anomalous neutral triple gauge boson couplings are derived.
Search for New Phenomena in Tt Events with Large Missing Transverse Momentum in Proton-proton Collisions at Sqrt[s] = 7 TeV with the ATLAS Detector
A search for new phenomena in tt events with large missing transverse momentum in proton-proton collisions at a center-of-mass energy of 7 TeV is presented. The measurement is based on 1.04 fb(-1) of data collected with the ATLAS detector at the LHC. Contributions to this final state may arise from a number of standard model extensions. The results are interpreted in terms of a model where new top-quark partners are pair produced and each decay to an on-shell top (or antitop) quark and a long-lived undetected neutral particle. The data are found to be consistent with standard model expectations. A limit at 95% confidence level is set excluding a cross section times branching ratio of 1.1 pb for a top-partner mass of 420 GeV and a neutral particle mass less than 10 GeV. In a model of exotic fourth generation quarks, top-partner masses are excluded up to 420 GeV and neutral particle masses up to 140 GeV.
Measurements of the Angular Distributions in the Decays B→K^{(*)}μ^{+}μ^{-} at CDF
We report an indirect search for nonstandard model physics using the flavor-changing neutral current decays B→K^{(*)}μ^{+}μ^{-}. We reconstruct the decays and measure their angular distributions, as a function of q^{2}=M_{μμ}^{2}c^{2}, where M_{μμ} is the dimuon mass, in pp[over ¯] collisions at sqrt[s]=1.96  TeV using a data sample corresponding to an integrated luminosity of 6.8  fb^{-1}. The transverse polarization asymmetry A_{T}^{(2)} and the time-reversal-odd charge-and-parity asymmetry A_{im} are measured for the first time, together with the K^{*} longitudinal polarization fraction F_{L} and the muon forward-backward asymmetry A_{FB} for the decays B^{0}→K^{*0}μ^{+}μ^{-} and B^{+}→K^{*+}μ^{+}μ^{-}. The B→K^{*}μ^{+}μ^{-} forward-backward asymmetry in the most sensitive kinematic regime, 1≤q^{2}<6  GeV^{2}/c^{2}, is measured to be A_{FB}=0.29_{-0.23}^{+0.20}(stat)±0.07(syst), the most precise result to date. No deviations from the standard model predictions are observed.
Search for the Higgs Boson in the H→WW(*)→ℓ(+)νℓ(-)ν Decay Channel in Pp Collisions at ˆšs=7 TeV with the ATLAS Detector
A search for the Higgs boson has been performed in the H→WW(*)→ℓ(+)νℓ(-)ν[over ¯] channel (ℓ=e/μ) with an integrated luminosity of 2.05 fb(-1) of pp collisions at √s=7 TeV collected with the ATLAS detector at the Large Hadron Collider. No significant excess of events over the expected background is observed and limits on the Higgs boson production cross section are derived for a Higgs boson mass in the range 110 GeV
Search for the Standard Model Higgs Boson in the Diphoton Decay Channel with 4.9 Fb(-1) of Pp Collision Data at ˆšs=7 TeV with ATLAS
A search for the standard model Higgs boson is performed in the diphoton decay channel. The data used correspond to an integrated luminosity of 4.9 fb(-1) collected with the ATLAS detector at the Large Hadron Collider in proton-proton collisions at a center-of-mass energy of √s=7 TeV. In the diphoton mass range 110-150 GeV, the largest excess with respect to the background-only hypothesis is observed at 126.5 GeV, with a local significance of 2.8 standard deviations. Taking the look-elsewhere effect into account in the range 110-150 GeV, this significance becomes 1.5 standard deviations. The standard model Higgs boson is excluded at 95% confidence level in the mass ranges of 113-115 GeV and 134.5-136 GeV.
Observation of a New χ(b) State in Radiative Transitions to Υ(1S) and Υ(2S) at ATLAS
The χ(b)(nP) quarkonium states are produced in proton-proton collisions at the Large Hadron Collider at sqrt[s] = 7  TeV and recorded by the ATLAS detector. Using a data sample corresponding to an integrated luminosity of 4.4  fb(-1), these states are reconstructed through their radiative decays to Υ(1S,2S) with Υ → μ+ μ-. In addition to the mass peaks corresponding to the decay modes χ(b)(1P,2P) → Υ(1S)γ, a new structure centered at a mass of 10.530 ± 0.005(stat) ± 0.009(syst)  GeV is also observed, in both the Υ(1S)γ and Υ(2S)γ decay modes. This structure is interpreted as the χ(b)(3P) system.
