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In JoVE (1)
- High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Other Publications (7)
Articles by Tal Imbar in JoVE
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Felix Moltzahn1,2,3, Nathan Hunkapiller1,2,4, Alain A. Mir5, Tal Imbar1,2,6, Robert Blelloch1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco
Here we describe an optimized multiplex reverse transcriptase quantitative PCR (qRT-PCR) protocol in combination with a microfluidic platform as a cost and time effective high-throughput screening tool for microRNA (miRNA) expression levels, especially when working with limited amounts of sample.
Published August 3, 2011. Keywords: Bioengineering, microRNA, multiplex qRT-PCR, high throughput profiling, Fluidigm
Other articles by Tal Imbar on PubMed
Uterine Artery Embolization to Control Hemorrhage After Termination of Pregnancy Implanted in a Cesarean Delivery Scar
Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine. Oct, 2003 | Pubmed ID: 14606570
Fertility and Sterility. Aug, 2004 | Pubmed ID: 15302305
To evaluate the need of P for endometrial preparation before cryopreserved embryo transfer (ET) in an artificial cycle.
The Significance of Intrauterine Lesions Detected by Ultrasound in Asymptomatic Postmenopausal Patients
BJOG : an International Journal of Obstetrics and Gynaecology. Mar, 2005 | Pubmed ID: 15713160
A retrospective study on 82 women with an incidental sonographic finding suspected to be intrauterine polyps was undertaken to assess the histopathologic characteristics of such polyps utilising operative hysteroscopy. Endometrial polyps were found in 68 patients, submucousal myomas in 7, atrophic endometrium in 6 and thickened proliferative endometrium was found in 1 patient. Simple hyperplasia was found in one polyp but neither endometrial carcinoma nor complex hyperplasia was found. The total complication rate was 3.6%. It appears that the risk of endometrial carcinoma in postmenopausal women with asymptomatic endometrial polyps is low, although a larger series is required to confirm this finding.
Diagnosis, Surveillance, and Treatment of the Anemic Fetus Using Middle Cerebral Artery Peak Systolic Velocity Measurement
Prenatal Diagnosis. Jan, 2006 | Pubmed ID: 16374898
The in utero course of the anemic fetus has improved dramatically, owing to early diagnosis and cordocentesis transfusion. In utero invasive procedures such as amnio- and cordocentesis have become important modalities in the evaluation and treatment of anemic fetuses. However, they carry risks for both the mother and fetus. A valid and sensitive noninvasive means of following the anemic fetus is the evaluation of changes in the middle cerebral artery peak systolic flow velocity (MCA-PSV). This is a sensitive tool for both the evaluation of fetal anemia and response to treatment. Intracerebral vessels respond earliest to the fetal anemic state, and are readily accessible for ultrasound examination. We describe the methodology and evolving clinical applications of MCA-PSV measurement in the fetus, through an overview of the literature describing the development and application of MCA-PSV measurement in fetuses at risk of fetal anemia of various immune and nonimmune etiologies, illustrated by index cases from our center. MCA-PSV measurement is essential in the diagnosis, evaluation, and management of cases of fetal anemia. The use of this modality lessens the need for invasive procedures. The method is readily accessible and should be integrated into the repertoire of all obstetric ultrasound centers.
Fertility and Sterility. Nov, 2008 | Pubmed ID: 18440000
To present the set of reasons for and against fertility treatment for a very young patient.
Fertility and Sterility. Jan, 2009 | Pubmed ID: 18249376
To determine the predictive value and the quality of supernatant sperm (SS) achieved by a simple laboratory technical modification after testicular sperm extraction (TESE).
Reproductive Outcome of Fresh or Frozen-thawed Embryo Transfer is Similar in High-risk Patients for Ovarian Hyperstimulation Syndrome Using GnRH Agonist for Final Oocyte Maturation and Intensive Luteal Support
Human Reproduction (Oxford, England). Mar, 2012 | Pubmed ID: 22252086
BACKGROUND Triggering ovulation by GnRH agonist (GnRHa) in GnRH antagonist IVF protocols coupled with adequate luteal phase support has recently been suggested as a means to prevent ovarian hyperstimulation syndrome (OHSS). Our objective was to examine the outcome of fresh embryo transfer (f-ET) after triggering ovulation by GnRHa and providing intensive luteal phase supplementation, compared with that of the next first frozen-thawed embryo transfer (ft-ET) after cycles with the same protocol and cryopreservation of all the embryos. METHODS We performed a cohort study at a university-based IVF clinic. The study population was patients at high risk for OHSS. A daily dose of 50 mg i.m. progesterone in oil and 6 mg of oral 17-Î²-estradiol initiated on oocyte retrieval day in the f-ET group (n= 70). In the ft-ET group (n= 40) the embryos were cryopreserved and transferred in the next cycle. RESULTS The live birth rate per f-ET was 27.1 versus 20% in the ft-ET groups [P = 0.4; rate ratio = 1.36 (0.65-2.81)]. The implantation, pregnancy and spontaneous abortion rates were comparable in both groups. None of the patients developed OHSS. CONCLUSIONS In this observational cohort study, we showed that triggering ovulation with GnRHa and intensive luteal phase support is a promising new modality to prevent OHSS without the cost of cycle cancellation, ET deferral and reduced clinical pregnancy rates. Confirmation of these findings by RCTs is now required.