Translate this page to:
Swedish
In JoVE (1)
Other Publications (1)
Automatic Translation
This translation into Swedish was automatically generated.
English Version | Other Languages
Articles by Thomas Gietzelt in JoVE
JoVE General
Mikrofabrikation av Chip-storlek Byggnadsställningar för tredimensionell cellodling
1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre
Vi presenterar två processer för mikrofabrikationslaboratorier av porösa polymera marker för tredimensionell cellodling. Den första är varmpressning kombineras med en lösningsmedelsångor svetsning. Den andra använder en nyligen utvecklad microthermoforming processen kombinerat med ion spåret teknik som leder till en betydande förenkling av tillverkningen.
Other articles by Thomas Gietzelt on PubMed
A Chip-based Platform for the in Vitro Generation of Tissues in Three-dimensional Organization
We describe a multi-purpose platform for the three-dimensional cultivation of tissues. The device is composed of polymer chips featuring a microstructured area of 1-2 cm(2). The chip is constructed either as a grid of micro-containers measuring 120-300 x 300 x 300 microm (h x l x w), or as an array of round recesses (300 microm diameter, 300 microm deep). The micro-containers may be separately equipped with addressable 3D-micro-electrodes, which allow for electrical stimulation of excitable cells and on-site measurements of electrochemically accessible parameters. The system is applicable for the cultivation of high cell densities of up to 8 x 10(6) cells and, because of the rectangular grid layout, allows the automated microscopical analysis of cultivated cells. More than 1000 micro-containers enable the parallel analysis of different parameters under superfusion/perfusion conditions. Using different polymer chips in combination with various types of bioreactors we demonstrated the principal suitability of the chip-based bioreactor for tissue culture applications. Primary and established cell lines have been successfully cultivated and analysed for functional properties. When cells were cultured in non-perfused chips, over time a considerable degree of apoptosis could be observed indicating the need for an active perfusion. The system presented here has also been applied for the differentiation analysis of pluripotent embryonic stem cells and may be suitable for the analysis of the stem cell niche.
