Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

JoVE 1931 7/28/2010

Huy Nguyen¹, Cristy Loustaunau¹, Alexander Facista¹, Lois Ramsey¹, Nadia Hassounah¹, Hilary Taylor¹, Robert Krouse^2,3, Claire M. Payne^1,4, V. Liana Tsikitis³, Steve Goldschmid⁵, Bhaskar Banerjee⁵, Rafael F. Perini⁵, Carol Bernstein¹

¹Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, ²Southern Arizona Veterans Affairs Health Care System, Tucson, AZ, ³Department of Surgery, College of Medicine, University of Arizona, Tucson, ⁴Biomedical Diagnostics and Research, Tucson, AZ, ⁵Department of Medicine, College of Medicine, University of Arizona, Tucson

Reduced/absent expression of Pms2 and/or ERCC1 in entire crypts is a frequent event within 10 cm on each side of colonic adenocarcinomas, likely the basis of a field defect with high mutability and progression to cancer. Deficiency in Ku86 or CcOI is much less frequent in these field defects.