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In JoVE (3)
- Proper Care and Cleaning of the Microscope
- Major Components of the Light Microscope
- Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Other Publications (1)
Articles by Victoria Centonze Frohlich in JoVE
JoVE General
Proper Care and Cleaning of the Microscope
Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
JoVE General
Major Components of the Light Microscope
Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
JoVE General
Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)
This protocol highlights the principles and practical applications of Phase and Differential Interference Contrast (DIC) Microscopy
Other articles by Victoria Centonze Frohlich on PubMed
Single Cell Behavior in Metastatic Primary Mammary Tumors Correlated with Gene Expression Patterns Revealed by Molecular Profiling
We have developed animal models of breast cancer that allow the direct examination of the behavior of individual green fluorescent protein-expressing carcinoma cells in live nonmetastatic and metastatic primary tumors in situ. We have combined this model with multiphoton microscopy to image differences in cell behavior within the primary tumor. Differences in cell behavior between nonmetastatic and metastatic cells in culture and within live primary tumors were correlated with results from cDNA microarray analyses to identify potentially important genetic determinants for breast cancer invasion and metastasis. Using multiphoton microscopy, we found five major differences in carcinoma cell behavior between the nonmetastatic and metastatic primary breast tumors involving extracellular matrix, cell motility, and chemotaxis. Behavioral differences were correlated with seven categories of molecules that were differentially expressed and related to these behaviors. We have found that extracellular matrix composition, actin nucleation factors, molecules involved in mechanical stability and survival, and cell polarity and chemotaxis showed large and consistent differences in gene expression. We conclude that aligning cell behavior in vivo with patterns of gene expression can lead to new insights into the microenvironment of carcinoma cells in the primary tumor and the molecular mechanisms behind cell behavior.
