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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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In JoVE (1)

Other Publications (20)

Articles by Volker Dürr in JoVE

 JoVE Neuroscience

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)

1Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University


JoVE 50179

In this protocol we show how to condition harnessed honey bees to tactile stimuli and introduce a 2D motion capture technique for analyzing the kinematics of fine-scale antennal sampling pattern.

Other articles by Volker Dürr on PubMed

Sexual Dysfunction Related to the Treatment of Young Women with Breast Cancer

Women have a 13.4% chance of developing breast cancer over their lifetime, and an estimated 60,000 women aged 20-50 will develop breast cancer in 2005. Surgical, radiologic, and chemotherapeutic treatments used to treat breast cancer can alter women's body image and menopausal status, ultimately affecting sexual health. A diagnosis of breast cancer is more traumatic for young women because of psychosocial concerns, side effects of treatment, and a potentially shortened life expectancy. Alterations in sexual health interfere with intimate relationships. Nurses can play a pivotal role in improving the sexual health of young women with breast cancer. This article explores the potential side effects of treatment that can affect sexual health, the unique needs of young women, and nursing interventions that systematically address sexual health concerns.

Nutrition Intervention Using an Eicosapentaenoic Acid (EPA)-containing Supplement in Patients with Advanced Colorectal Cancer. Effects on Nutritional and Inflammatory Status: a Phase II Trial

The aim of the study was to assess the impact of an eicosapentanoic acid-containing protein and energy dense oral nutritional supplement (EPA-ONS) on nutritional and inflammatory status, quality of life (QOL), plasma phospholipids (PPL) and cytokine profile, tolerance of irinotecan-containing chemotherapy and EPA-ONS in patients with advanced colorectal cancer (CRC) receiving chemotherapy.

Network Formation by Rhizomorphs of Armillaria Lutea in Natural Soil: Their Description and Ecological Significance

Armillaria lutea rhizomorphs in soil were mapped over areas of 25 m2 at a Pinus nigra (site I) and a Picea abies (site II) plantation. Rhizomorph density was 4.3 and 6.1 m m(-2) soil surface with 84% and 48% of the total rhizomorph length in the mapped area interconnected in a network at site I and site II, respectively. At site I there were only two network attachments to Pinus stumps, but at site II many more to Picea roots and stumps. Anastomoses of rhizomorphs resulted in cyclic paths, parts of the network that start and end at the same point. Connections between different rhizomorph segments were shown to allow gaseous exchange. The network at site I consisted of 169 rhizomorphs ('edges'), and 107 rhizomorph nodes ('vertices'). Disruption of two critical edges ('bridges') would lead to large parts (13% and 11%) being disconnected from the remainder of the mapped network. There was a low probability that amputation of a randomly chosen edge would separate the network into two disconnected components. The high level of connectedness may enhance redistribution of nutrients and provide a robust rhizomorph structure, allowing Armillaria to respond opportunistically to spatially and temporally changing environments.

What Constitutes a Dignified Death? The Voice of Oncology Advanced Practice Nurses

The purpose of this study was to explore the concept of dignified dying from the perspective of oncology advanced practice nurses.

Developing a List of Reference Chemicals for Testing Alternatives to Whole Fish Toxicity Tests

This paper details the derivation of a list of 60 reference chemicals for the development of alternatives to animal testing in ecotoxicology with a particular focus on fish. The chemicals were selected as a prerequisite to gather mechanistic information on the performance of alternative testing systems, namely vertebrate cell lines and fish embryos, in comparison to the fish acute lethality test. To avoid the need for additional experiments with fish, the U.S. EPA fathead minnow database was consulted as reference for whole organism responses. This database was compared to the Halle Registry of Cytotoxicity and a collation of data by the German EPA (UBA) on acute toxicity data derived from zebrafish embryos. Chemicals that were present in the fathead minnow database and in at least one of the other two databases were subject to selection. Criteria included the coverage of a wide range of toxicity and physico-chemical parameters as well as the determination of outliers of the in vivo/in vitro correlations. While the reference list of chemicals now guides our research for improving cell line and fish embryo assays to make them widely applicable, the list could be of benefit to search for alternatives in ecotoxicology in general. One example would be the use of this list to validate structure-activity prediction models, which in turn would benefit from a continuous extension of this list with regard to physico-chemical and toxicological data.

Living with Death and Dying: the Experience of Taiwanese Hospice Nurses

To explore and describe the experiences of Taiwanese nurses who care for dying patients in hospices, a relatively recent healthcare option in Taiwan.

The Use of Theory in Qualitative Approaches to Research: Application in End-of-life Studies

This paper is a report of an analysis of the use of theory in qualitative approaches to research as exemplified in qualitative end-of-life studies.

Women's Experiences As Members of Attention Control and Experimental Intervention Groups in a Randomized Controlled Trial

Attention control groups are often used in research testing the efficacy of psychosocial and behavioural interventions in order to control for placebo effects. The authors conducted a descriptive qualitative study to investigate how participants viewed their experiences in attention control and experimental intervention groups following a randomized controlled trial for women with fibromyalgia syndrome. Moderately structured interviews were conducted with 18 women (12 from the experimental intervention group and 6 from the attention control group). Members of the control group reported some benefits but few behavioural changes as a result of participating in the RCT, and some participants expressed disappointment at not receiving the intervention. Perceptions of changes in attitudes towards fibromyalgia syndrome and behaviours reported by the intervention group appear to be consistent with the theory underlying the intervention. Possible placebo effects identified in both groups include negative and positive social interactions with other participants.

The Meaning of Surviving Cancer for Latino Adolescents and Emerging Young Adults

Adolescent and young adult (AYA) survivors of cancer are an understudied population with unique developmental and medical needs that extend well beyond their active treatment. Survivors diagnosed as AYAs may experience both physical and emotional late effects. In particular, the experiences of Latino cancer survivors have not been explored. The purpose of this study was to conduct interviews with AYA Latino cancer survivors to inform professionals working with these survivors. A hermeneutic phenomenological approach was selected based on the focus on experiences and meanings of Latino adolescents' cancer survivorship. Phenomenology allows for understanding the subjective meaning and lived experience of populations that are understudied or marginalized. In-depth interviews were conducted with participants. Enrolled in the study were Latino AYAs between the ages of 14 and 21 years, after treatment. Interviews revealed 7 themes regarding the experience and meaning of survivorship for this population: gratitude, humor/positive attitude, empathy for younger children with cancer, God and faith, cancer happens for a reason/cancer changed my life, familial support, and staff relationships. Latino AYA cancer survivors develop meaning out of unique cancer experiences. Programs need to be developed specifically to address Latino adolescents and young adult survivors of cancer.

Classroom Performance System Use in an Accelerated Graduate Nursing Program

Many students who enter accelerated nursing programs have not been exposed to the analysis, prediction, and decision-making skills needed by today's RN. To foster practice with complex concepts in the classroom and to give teachers immediate feedback about student in-class mastery of core material, use of an audience participation system within the classroom may be useful. This article reports the implementation of a classroom performance system and the results ofa program evaluation project designed to capture the system's impact on student and faculty satisfaction and student learning outcomes. Project results and implications for further work are presented.

Cervical Cancer Screening and Older Mexican American Women

The purpose of this study was to explore an older Mexican American woman's decision-making process to engage in cervical cancer screening. A qualitative single case study design was used along with a purposive, typical case sampling strategy. The participant, a 52-year-old Mexican American woman, was interviewed using a semi-structured format. Qualitative content analysis was used to analyze the data. The analytic process revealed three concepts and motivators that influenced the participant's behavior regarding cervical cancer screening practices: knowledge, family history, and sexual history. As such, these findings are useful for crafting subsequent investigations. Although the study participant's experience is instructive regarding facilitators or motivators for engaging in screening practices, further exploration of barriers faced by older Mexican American women who decline to be screened is warranted.

Assessing Cancer-related Learning Needs of Texas Nurses

Given the growing number of cancer survivors, all nurses must have current knowledge and skills to provide competent cancer care. Accordingly, access to evidence-based educational opportunities designed to promote ongoing competency must be ensured. Program offerings and services should be based on a systematic and periodic approach to provide appropriate programming that meets learners' self-identified needs, priorities, and self-reported gaps in existing knowledge and practice.

Pantothenic Acid Supplementation to Support Rumen Microbes?

Based on repeatedly reported extensive pantothenic acid disappearance in the rumen, the present study is aimed at examining if pantothenic acid is used for a more efficient ruminal fermentation and microbial growth in an artificial rumen (Rusitec). Three substrates differing in roughage/concentrate ratio were incubated with and without the addition of Ca-D-pantothenate. Pantothenic acid was extensively degraded without notably influencing fermentation, microbial protein synthesis and the status of other B-vitamins such as riboflavin, vitamin B6 and niacin. Therefore, pantothenic acid supplementation cannot be expected to contribute to microbial benefit for the ruminant animal.

Cancer Patients' Preferences for Control at the End of Life

The achievement of a death consistent with personal preferences is an elusive outcome for most people with cancer. Maintaining a sense of control is a core component of a dignified death; however, control might be a Western bioethical notion with questionable relevance to culturally diverse groups. Thus, the purpose of our study was to explore the meaning of control and control preferences in a group of racially and ethnically diverse patients with an advanced cancer diagnosis. Using a hermeneutic, phenomenological approach, we interviewed 20 patients with advanced cancer and uncovered two themes: (a) preferences for everyday control over treatment decisions, family issues, final days of life, and arrangements after death, vs. (b) awareness that cancer and death are controlled by a higher power. Although the sample included non-Hispanic Whites, African Americans, and Hispanics, participants shared common views that are characteristic of American cultural norms regarding the value of autonomy.

Randomised Controlled Trial of Tailored Interventions to Improve the Management of Anxiety and Depressive Disorders in Primary Care

ABSTRACT:

Best Practice Elements of Multilevel Suicide Prevention Strategies: a Review of Systematic Reviews

Evidence-based best practices for incorporation into an optimal multilevel intervention for suicide prevention should be identifiable in the literature.

A Narrative Study of Women's Early Symptom Experience of Ischemic Stroke

Delayed arrival at the emergency department after the onset of ischemic stroke symptoms is an important reason for low tissue plasminogen activator administration rates. There is evidence that women arrive at the hospital later than do men, but little is known about women's experiences in the period between symptom onset and hospital arrival. The purpose of this naturalistic investigation using narrative methodology was to gain understanding of women's early symptom experience of ischemic stroke.

Humanistic Nursing Theory: Application to Hospice and Palliative Care

This article presents a discussion of the relevance of Humanistic Nursing Theory to hospice and palliative care nursing.

End of Life and Women Aging with a Disability

Approximately 21 million noninstitutionalized Americans with physical disabilities will ultimately face end-of-life [EOL] issues. Studies have documented disparate care and poorer outcomes for persons with preexisting disabilities who have life-limiting illnesses, which raises the question of how EOL experiences may differ for these individuals. The aim of this qualitative, descriptive study was to explore how EOL issues might emerge within the life stories of women aging with functional disabilities. Interview data were obtained from a larger, ongoing ethnographic study focused on the creation of an explanatory model of health disparities of disablement in women with mobility impairment. Each participant was interviewed three to four times using a life-course perspective that captures life trajectories and transitions experienced over time. For this analysis, 41 interviews were selected from 20 participants who discussed issues related to death and dying. Content analysis of the data revealed five analytic categories: death as a signpost, impact of others' deaths, deaths that affected personal insights and choice, EOL possibilities, and a personal brush with death. EOL issues were manifested in a variety of ways that revealed both determination to remain as independent as possible within the context of declining functional ability and uncertainty regarding the future.

Caring for Students With Type 1 Diabetes: School Nurses' Experiences

This qualitative study used a Husserlian phenomenological approach to obtain an understanding of the essences of five experienced Taiwanese school nurses' lived experience of caring for students with type 1 diabetes mellitus (T1DM). Audio-recorded, semi-structured, in-depth interviews were conducted. Data analysis entailed a modified method from Colaizzi. Four intertwined themes were discovered: (a) I try to put myself in the parents' and students' shoes, (b) I am not a diabetes expert, (c) managing T1DM requires teamwork, and (d) caring for students with T1DM is a struggle with practical limitations. The findings show that these school nurses encountered many challenges as they implemented their roles and responsibilities in caring for students with T1DM. The findings suggest that increasing school nurses' competence in caring for students with T1DM and developing effective strategies to overcome the challenges faced may be useful. Multidisciplinary teamwork could benefit the diabetes management activities in school settings.

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