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In JoVE (1)
Other Publications (1)
Articles by WP Andrew Lee in JoVE
Orthotopic Hind-Limb Transplantation in Rats
Robert Sucher*1, Rupert Oberhuber*1, Christian Margreiter1, Guido Rumberg1, Rishi Jindal2, WP Andrew Lee2, Raimund Margreiter1, Johann Pratschke1, Stefan Schneeberger1, Gerald Brandacher1
1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center
Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.
Other articles by WP Andrew Lee on PubMed
Regenerative Medicine. Nov, 2011 | Pubmed ID: 22050522
AIMS: Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand-forelimb transplants performed at the University of Pittsburgh (PA, USA). MATERIALS & METHODS: The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute's Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).