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In JoVE (2)
- עצמיים אבי האנדותל Cell-מתזמן טכנולוגיה בדיקה biocompatibility עבור התקנים לב וכלי דם במודל חיה גדולה
- במקביל, צלחת תזרים לשכת ואת מעגל זרימה רציפה להערכת ובתאים אנדותל תחת מתח זרימה למינרית שאר
Other Publications (2)
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Articles by Whitney O. Lane in JoVE
JoVE Bioengineering
עצמיים אבי האנדותל Cell-מתזמן טכנולוגיה בדיקה biocompatibility עבור התקנים לב וכלי דם במודל חיה גדולה
1Department of Biomedical Engineering, Duke University, 2School of Medicine, Duke University, 3Department of Surgery, Duke University Medical Center, 4School of Medicine, University of Pennsylvania
שיטה טיטניום זריעת דם biomaterials ליצור קשר עם תאים עצמיים ו biocompatibility בדיקות מתואר. שיטה זו משתמשת ובתאים אנדותל צינורות טיטניום, זורעים בתוך דקות של ניתוח ההשתלה לתוך חזירי venae cavae. טכניקה זו ניתנת להתאמה מכשירים רבים אחרים ביו מושתלת.
JoVE Bioengineering
במקביל, צלחת תזרים לשכת ואת מעגל זרימה רציפה להערכת ובתאים אנדותל תחת מתח זרימה למינרית שאר
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center
אנחנו המתאר שיטה תאים חסיד נתונים ללחץ הזרימה למינרית גזירה במעגל תזרים סטרילי מתמשך. הדבקה של התאים, מורפולוגיה ניתן ללמוד דרך תא שקוף, דגימות שהתקבלו במעגל לניתוח המטבוליט ותאי שנקטפו לאחר חשיפה גזירה לניסויים עתידיים או תרבות.
Other articles by Whitney O. Lane on PubMed
The Biocompatibility of Titanium Cardiovascular Devices Seeded with Autologous Blood-derived Endothelial Progenitor Cells: EPC-seeded Antithrombotic Ti Implants
Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with an antithrombogenic coating that mimics the native lining of blood vessels and the heart. We evaluated the viability and adherence of peripheral blood-derived porcine endothelial progenitor cells (EPCs), seeded onto thin Ti layers on glass slides under static conditions and after exposure to fluid shear stresses. EPCs attached and grew to confluence on Ti in serum-free medium, without preadsorption of proteins. After attachment to Ti for 15 min, less than 5% of the cells detached at a shear stress of 100 dyne / cm(2). Confluent monolayers of EPCs on smooth Ti surfaces (Rq of 10 nm), exposed to 15 or 100 dyne/cm(2) for 48 h, aligned and elongated in the direction of flow and produced nitric oxide dependent on the level of shear stress. EPC-coated Ti surfaces had dramatically reduced platelet adhesion when compared to uncoated Ti surfaces. These results indicate that peripheral blood-derived EPCs adhere and function normally on Ti surfaces. Therefore EPCs may be used to seed cardiovascular devices prior to implantation to ameliorate platelet activation and thrombus formation.
Use of Autologous Blood-derived Endothelial Progenitor Cells at Point-of-care to Protect Against Implant Thrombosis in a Large Animal Model
Titanium (Ti) is commonly utilized in many cardiovascular devices, e.g. as a component of Nitinol stents, intra- and extracorporeal mechanical circulatory assist devices, but is associated with the risk of thromboemboli formation. We propose to solve this problem by lining the Ti blood-contacting surfaces with autologous peripheral blood-derived late outgrowth endothelial progenitor cells (EPCs) after having previously demonstrated that these EPCs adhere to and grow on Ti under physiological shear stresses and functionally adapt to their environment under flow conditions ex vivo. Autologous fluorescently-labeled porcine EPCs were seeded at the point-of-care in the operating room onto Ti tubes for 30 min and implanted into the pro-thrombotic environment of the inferior vena cava of swine (n = 8). After 3 days, Ti tubes were explanted, disassembled, and the blood-contacting surface was imaged. A blinded analysis found all 4 cell-seeded implants to be free of clot, whereas 4 controls without EPCs were either entirely occluded or partially thrombosed. Pre-labeled EPCs had spread and were present on all 4 cell-seeded implants while no endothelial cells were observed on control implants. These results suggest that late outgrowth autologous EPCs represent a promising source of lining Ti implants to reduce thrombosis in vivo.
