Wolfgang Wiechert IBG-1: Biotechnology Forschungszentrum Jülich Biography Publications Institution JoVE Articles Wolfgang Wiechert has not added a biography. If you are Wolfgang Wiechert and would like to personalize this page please email our Author Liaison for assistance. Publications Automated Growth Rate Determination in High-throughput Microbioreactor Systems BMC Research Notes. Nov, 2017 | Pubmed ID: 29178966 Laboratory Scale Photobiotechnology - Current Trends and Future Perspectives FEMS Microbiology Letters. Nov, 2017 | Pubmed ID: 29126108 PH Fluctuations Imperil the Robustness of C. Glutamicum to Short Term Oxygen Limitation Journal of Biotechnology. Oct, 2017 | Pubmed ID: 28837821 Corynebacterium Glutamicum Chassis C1*: Building and Testing a Novel Platform Host for Synthetic Biology and Industrial Biotechnology ACS Synthetic Biology. Aug, 2017 | Pubmed ID: 28803482 To Be Certain About the Uncertainty: Bayesian Statistics for 13 C Metabolic Flux Analysis Biotechnology and Bioengineering. Nov, 2017 | Pubmed ID: 28695999 Homogenizing Bacterial Cell Factories: Analysis and Engineering of Phenotypic Heterogeneity Metabolic Engineering. Jul, 2017 | Pubmed ID: 28645641 Miniaturized and Automated Adaptive Laboratory Evolution: Evolving Corynebacterium Glutamicum Towards an Improved D-xylose Utilization Bioresource Technology. Dec, 2017 | Pubmed ID: 28552568 Fast and Reliable Strain Characterization of Streptomyces Lividans Through Micro-scale Cultivation Biotechnology and Bioengineering. Sep, 2017 | Pubmed ID: 28436005 Coarse-graining Bacteria Colonies for Modelling Critical Solute Distributions in Picolitre Bioreactors for Bacterial Studies on Single-cell Level Microbial Biotechnology. Jul, 2017 | Pubmed ID: 28371389 A Framework for Accelerated Phototrophic Bioprocess Development: Integration of Parallelized Microscale Cultivation, Laboratory Automation and Kriging-assisted Experimental Design Biotechnology for Biofuels. 2017 | Pubmed ID: 28163783 Comparative Evaluation of Phototrophic Microtiter Plate Cultivation Against Laboratory-scale Photobioreactors Bioprocess and Biosystems Engineering. May, 2017 | Pubmed ID: 28101644 Metabolic Profile of 1,5-diaminopentane Producing Corynebacterium Glutamicum Under Scale-down Conditions: Blueprint for Robustness to Bioreactor Inhomogeneities Biotechnology and Bioengineering. Mar, 2017 | Pubmed ID: 27641904 Design and Validation of a Parallelized Micro-photobioreactor Enabling Phototrophic Bioprocess Development at Elevated Throughput Biotechnology and Bioengineering. Jan, 2017 | Pubmed ID: 27424867 Use of a Sec Signal Peptide Library from Bacillus Subtilis for the Optimization of Cutinase Secretion in Corynebacterium Glutamicum Microbial Cell Factories. Dec, 2016 | Pubmed ID: 27927208 A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid L-Lysine Analysis Sensors (Basel, Switzerland). Sep, 2016 | Pubmed ID: 27690044 Image-Based Single Cell Profiling: High-Throughput Processing of Mother Machine Experiments PloS One. 2016 | Pubmed ID: 27661996 Time-resolved, Single-cell Analysis of Induced and Programmed Cell Death Via Non-invasive Propidium Iodide and Counterstain Perfusion Scientific Reports. Sep, 2016 | Pubmed ID: 27580964 μMORE: A Microfluidic Magnetic Oscillation Reactor for Accelerated Parameter Optimization in Biocatalysis Journal of Biotechnology. Aug, 2016 | Pubmed ID: 27288595 Automation of a Nile Red Staining Assay Enables High Throughput Quantification of Microalgal Lipid Production Microbial Cell Factories. Feb, 2016 | Pubmed ID: 26861538 Discrete-continuous Reaction-diffusion Model with Mobile Point-like Sources and Sinks The European Physical Journal. E, Soft Matter. Jan, 2016 | Pubmed ID: 26830760 A Scientific Workflow Framework for (13)C Metabolic Flux Analysis Journal of Biotechnology. Aug, 2016 | Pubmed ID: 26721184 Photocaged Arabinose: A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression Chembiochem : a European Journal of Chemical Biology. Feb, 2016 | Pubmed ID: 26677142 Cutting the Gordian Knot: Identifiability of Anaplerotic Reactions in Corynebacterium Glutamicum by Means of (13) C-metabolic Flux Analysis Biotechnology and Bioengineering. Mar, 2016 | Pubmed ID: 26375179 Non-Invasive Microbial Metabolic Activity Sensing at Single Cell Level by Perfusion of Calcein Acetoxymethyl Ester PloS One. 2015 | Pubmed ID: 26513257 (13)C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter Oxydans 621H Metabolites. Sep, 2015 | Pubmed ID: 26404385 Spatiotemporal Microbial Single-cell Analysis Using a High-throughput Microfluidics Cultivation Platform Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Dec, 2015 | Pubmed ID: 26348020 Modeling and CFD Simulation of Nutrient Distribution in Picoliter Bioreactors for Bacterial Growth Studies on Single-cell Level Lab on a Chip. Nov, 2015 | Pubmed ID: 26345659 Vizardous: Interactive Analysis of Microbial Populations with Single Cell Resolution Bioinformatics (Oxford, England). Dec, 2015 | Pubmed ID: 26261223 Live Cell Imaging of SOS and Prophage Dynamics in Isogenic Bacterial Populations Molecular Microbiology. Nov, 2015 | Pubmed ID: 26235130 The Effect of Composition on Diffusion of Macromolecules in a Crowded Environment Physical Biology. May, 2015 | Pubmed ID: 26020120 Bioprocess Automation on a Mini Pilot Plant Enables Fast Quantitative Microbial Phenotyping Microbial Cell Factories. Mar, 2015 | Pubmed ID: 25888907 How to Measure Metabolic Fluxes: a Taxonomic Guide for (13)C Fluxomics Current Opinion in Biotechnology. Aug, 2015 | Pubmed ID: 25531408 Absolute Quantification of Corynebacterium Glutamicum Glycolytic and Anaplerotic Enzymes by QconCAT Journal of Proteomics. Jan, 2015 | Pubmed ID: 25451015 Visual Workflows for 13C-metabolic Flux Analysis Bioinformatics (Oxford, England). Feb, 2015 | Pubmed ID: 25297067 Chassis Organism from Corynebacterium Glutamicum--a Top-down Approach to Identify and Delete Irrelevant Gene Clusters Biotechnology Journal. Feb, 2015 | Pubmed ID: 25139579 Engineering of Corynebacterium Glutamicum for Minimized Carbon Loss During Utilization of D-xylose Containing Substrates Journal of Biotechnology. Dec, 2014 | Pubmed ID: 25304460 An Evaluation of Genetically Encoded FRET-based Biosensors for Quantitative Metabolite Analyses in Vivo Journal of Biotechnology. Dec, 2014 | Pubmed ID: 25107505 Rapid Assessment of Oxygen Transfer Impact for Corynebacterium Glutamicum Bioprocess and Biosystems Engineering. Dec, 2014 | Pubmed ID: 24981020 Quantitative Metabolomics: a Phantom? Trends in Biotechnology. May, 2014 | Pubmed ID: 24708998 Developing a New Production Host from a Blueprint: Bacillus Pumilus As an Industrial Enzyme Producer Microbial Cell Factories. Mar, 2014 | Pubmed ID: 24661794 Single-cell Microfluidics: Opportunity for Bioprocess Development Current Opinion in Biotechnology. Oct, 2014 | Pubmed ID: 24642389 Process Inhomogeneity Leads to Rapid Side Product Turnover in Cultivation of Corynebacterium Glutamicum Microbial Cell Factories. Jan, 2014 | Pubmed ID: 24410842 Assessment of Robustness Against Dissolved Oxygen/substrate Oscillations for C. Glutamicum DM1933 in Two-compartment Bioreactor Bioprocess and Biosystems Engineering. Nov, 2013 | Pubmed ID: 24218302 Microfluidic Growth Chambers with Optical Tweezers for Full Spatial Single-cell Control and Analysis of Evolving Microbes Journal of Microbiological Methods. Sep, 2013 | Pubmed ID: 24041615 Beyond Growth Rate 0.6: What Drives Corynebacterium Glutamicum to Higher Growth Rates in Defined Medium Biotechnology and Bioengineering. Aug, 2013 | Pubmed ID: 23996851 13C-flux Spectral Analysis of Host-pathogen Metabolism Reveals a Mixed Diet for Intracellular Mycobacterium Tuberculosis Chemistry & Biology. Aug, 2013 | Pubmed ID: 23911587 Simultaneous Utilization of Glucose and Gluconate in Penicillium Chrysogenum During Overflow Metabolism Biotechnology and Bioengineering. Dec, 2013 | Pubmed ID: 23775209 Isotopically Non-stationary Metabolic Flux Analysis: Complex Yet Highly Informative Current Opinion in Biotechnology. Dec, 2013 | Pubmed ID: 23623747 Metabolic Isotopomer Labeling Systems. Part III: Path Tracing Mathematical Biosciences. Jul, 2013 | Pubmed ID: 23507460 Combined Fluxomics and Transcriptomics Analysis of Glucose Catabolism Via a Partially Cyclic Pentose Phosphate Pathway in Gluconobacter Oxydans 621H Applied and Environmental Microbiology. Apr, 2013 | Pubmed ID: 23377928 13CFLUX2--high-performance Software Suite for (13)C-metabolic Flux Analysis Bioinformatics (Oxford, England). Jan, 2013 | Pubmed ID: 23110970 Beyond Growth Rate 0.6: Corynebacterium Glutamicum Cultivated in Highly Diluted Environments Biotechnology and Bioengineering. Jan, 2013 | Pubmed ID: 22890752 Error Propagation Analysis for Quantitative Intracellular Metabolomics Metabolites. Nov, 2012 | Pubmed ID: 24957773 Influence of Organic Solvents on Enzymatic Asymmetric Carboligations Advanced Synthesis & Catalysis. Oct, 2012 | Pubmed ID: 23349644 Extensive Exometabolome Analysis Reveals Extended Overflow Metabolism in Various Microorganisms Microbial Cell Factories. 2012 | Pubmed ID: 22963408 A Disposable Picolitre Bioreactor for Cultivation and Investigation of Industrially Relevant Bacteria on the Single Cell Level Lab on a Chip. May, 2012 | Pubmed ID: 22511122 Tailoring a Stabilized Variant of Hydroxynitrile Lyase from Arabidopsis Thaliana Chembiochem : a European Journal of Chemical Biology. Apr, 2012 | Pubmed ID: 22378361 Collisional Fragmentation of Central Carbon Metabolites in LC-MS/MS Increases Precision of ¹³C Metabolic Flux Analysis Biotechnology and Bioengineering. Mar, 2012 | Pubmed ID: 22012626 Optical Sensors for Monitoring Dynamic Changes of Intracellular Metabolite Levels in Mammalian Cells Nature Protocols. Nov, 2011 | Pubmed ID: 22036884 Comparative 13C Metabolic Flux Analysis of Pyruvate Dehydrogenase Complex-deficient, L-valine-producing Corynebacterium Glutamicum Applied and Environmental Microbiology. Sep, 2011 | Pubmed ID: 21784914 The Benefits of Being Transient: Isotope-based Metabolic Flux Analysis at the Short Time Scale Applied Microbiology and Biotechnology. Sep, 2011 | Pubmed ID: 21732247 Visualizing Multi-omics Data in Metabolic Networks with the Software Omix: a Case Study Bio Systems. Aug, 2011 | Pubmed ID: 21575673 Mechanistic Pathway Modeling for Industrial Biotechnology: Challenging but Worthwhile Current Opinion in Biotechnology. Oct, 2011 | Pubmed ID: 21353523 Stationary Versus Non-stationary (13)C-MFA: a Comparison Using a Consistent Dataset Journal of Biotechnology. Jul, 2011 | Pubmed ID: 20638432 Modeling Languages for Biochemical Network Simulation: Reaction Vs Equation Based Approaches Advances in Biochemical Engineering/biotechnology. 2010 | Pubmed ID: 20309675 Translating Biochemical Network Models Between Different Kinetic Formats Metabolic Engineering. Mar, 2009 | Pubmed ID: 19013536 13C Labeling Experiments at Metabolic Nonstationary Conditions: an Exploratory Study BMC Bioinformatics. 2008 | Pubmed ID: 18366666 Visualizing Regulatory Interactions in Metabolic Networks BMC Biology. 2007 | Pubmed ID: 17939866 The Topology of Metabolic Isotope Labeling Networks BMC Bioinformatics. 2007 | Pubmed ID: 17727715 The Thermodynamic Meaning of Metabolic Exchange Fluxes Biophysical Journal. Sep, 2007 | Pubmed ID: 17526563 Fluxomics: Mass Spectrometry Versus Quantitative Imaging Current Opinion in Plant Biology. Jun, 2007 | Pubmed ID: 17481942 Nondestructive and Noncontact Method for Determining the Spring Constant of Rectangular Cantilevers The Review of Scientific Instruments. Apr, 2007 | Pubmed ID: 17477668 Metabolic Flux Analysis at Ultra Short Time Scale: Isotopically Non-stationary 13C Labeling Experiments Journal of Biotechnology. Apr, 2007 | Pubmed ID: 17207877 Computational Tools for Isotopically Instationary 13C Labeling Experiments Under Metabolic Steady State Conditions Metabolic Engineering. Nov, 2006 | Pubmed ID: 16890470 Experimental Design Principles for Isotopically Instationary 13C Labeling Experiments Biotechnology and Bioengineering. Jun, 2006 | Pubmed ID: 16598793 Interpretation of Metabolic Flux Maps by Limitation Potentials and Constrained Limitation Sensitivities Biotechnology and Bioengineering. Jun, 2006 | Pubmed ID: 16596665 Emerging Corynebacterium Glutamicum Systems Biology Journal of Biotechnology. Jun, 2006 | Pubmed ID: 16406159 From Stationary to Instationary Metabolic Flux Analysis Advances in Biochemical Engineering/biotechnology. 2005 | Pubmed ID: 15791936 Investigating the Dynamic Behavior of Biochemical Networks Using Model Families Bioinformatics (Oxford, England). Apr, 2005 | Pubmed ID: 15604106 Serial 13C-based Flux Analysis of an L-phenylalanine-producing E. Coli Strain Using the Sensor Reactor Biotechnology Progress. May-Jun, 2004 | Pubmed ID: 15176872 New Tools for Mass Isotopomer Data Evaluation in (13)C Flux Analysis: Mass Isotope Correction, Data Consistency Checking, and Precursor Relationships Biotechnology and Bioengineering. Feb, 2004 | Pubmed ID: 14748080 Metabolic Isotopomer Labeling Systems. Part II: Structural Flux Identifiability Analysis Mathematical Biosciences. Jun, 2003 | Pubmed ID: 12711410 MMT--a Pathway Modeling Tool for Data from Rapid Sampling Experiments In Silico Biology. 2002 | Pubmed ID: 12611627 An Introduction to 13C Metabolic Flux Analysis Genetic Engineering. 2002 | Pubmed ID: 12416307 Modeling and Simulation: Tools for Metabolic Engineering Journal of Biotechnology. Mar, 2002 | Pubmed ID: 11792451 Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology Johannes Hemmerich*1,2, Lars Freier*1,2, Wolfgang Wiechert1,2,3, Eric von Lieres1,2, Marco Oldiges1,2,4 1IBG-1: Biotechnology, Forschungszentrum Jülich, 2Research Center Jülich, Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 4Institute for Biotechnology, RWTH Aachen University JoVE 56234 Bioengineering Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation Alexander Gruenberger1, Christopher Probst1, Antonia Heyer1, Wolfgang Wiechert1, Julia Frunzke1, Dietrich Kohlheyer1 1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Juelich GmbH JoVE 50560 Bioengineering
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology Johannes Hemmerich*1,2, Lars Freier*1,2, Wolfgang Wiechert1,2,3, Eric von Lieres1,2, Marco Oldiges1,2,4 1IBG-1: Biotechnology, Forschungszentrum Jülich, 2Research Center Jülich, Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 4Institute for Biotechnology, RWTH Aachen University JoVE 56234 Bioengineering
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation Alexander Gruenberger1, Christopher Probst1, Antonia Heyer1, Wolfgang Wiechert1, Julia Frunzke1, Dietrich Kohlheyer1 1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Juelich GmbH JoVE 50560 Bioengineering
