JoVE Bioengineering
Carlos A. Lopez1, George G. Daaboul2, Sunmin Ahn2, Alexander P. Reddington1, Margo R. Monroe2, Xirui Zhang2, Rostem J. Irani3, Chunxiao Yu4,5, Caroline A. Genco4,5, Marina Cretich6, Marcella Chiari6, Bennett B. Goldberg1, John H. Connor5, M. Selim Ünlü1,2
1Department of Electrical and Computer Engineering, Boston University, 2Department of Biomedical Engineering, Boston University, 3Center for Advanced Genomics Technology, Boston University, 4Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 5Department of Microbiology, Boston University School of Medicine, 6CNR (National Research Council), Istituto di Chimica del Riconoscimento Molecolare
मात्रात्मक, उच्च throughput, वास्तविक समय, और लेबल मुक्त SiO पर biomolecular पता लगाने (डीएनए, प्रोटीन, आदि)
Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference. Aug, 2011 | Pubmed ID: 22255405
We utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation. A novel polymeric 3D scaffold is used to functionalize the sensor surface and to control orientation of the surface anchored DNA.