Articles by Yanming Du in JoVE

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

JoVE 3791 5/04/2012

Chen Lu¹, Joshua L. Wonsidler¹, Jianwei Li², Yanming Du¹, Timothy Block³, Brian Haab⁴, Songming Chen⁵

¹Institute for Hepatitis and Virus Research, ²Department of Microbiology and Immunology, Thomas Jefferson University, ³Drexel University College of Medicine, ⁴Van Andel Research Institute, ⁵Institute for Hepatitis and Virus Research, Serome Biosciences Inc.

In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.