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  JoVE Biology

  
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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Model Organisms II

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Essentials of
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 JoVE Biology

Pouring and Running a Protein Gel by reusing Commercial Cassettes

1Department of Biology, University of Florida, 2UF Genetics Institute, University of Florida, 3Plant Molecular & Cellular Biology Program, University of Florida


JoVE 3465

Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.

 JoVE Biology

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

1Research Animal Diagnostic Services (RADS), Charles River


JoVE 3715

Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.

 JoVE Biology

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

1Genetically Engineered Models and Services, Charles River, 2Research Models and Services, Charles River


JoVE 3713

Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.

 JoVE Biology

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

1BD Biosciences


JoVE 3418

Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.

 JoVE Biology

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

1Human Genetics, University of Michigan


JoVE 2625

The C. elegans embryo is a powerful system for studying cell biology and development. We present a protocol for live imaging of C. elegans embryos utilizing DIC optics or fluorescence using readily available epifluorescent microscopes and open-source software.

 JoVE Biology

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises

1Department of Biology, University of Kentucky, 2Department of Physiology, University of Toronto


JoVE 2325

This is a demonstration of how biological membranes can be understood using electrical models. We also demonstrate procedures for recording action potentials from the ventral nerve cord of the crayfish for student orientated laboratories.

 JoVE Biology

An Introduction to Worm Lab: from Culturing Worms to Mutagenesis

1Department of Biology, University of Texas at Arlington


JoVE 2293

Screening for mutants with phenotypic defects is a straightforward method for identifying genes that function in a given biological process. In this article we describe how to culture free living worms (e.g., Pristionchus pacificus) in the laboratory and show two different mutagenesis methods, EMS and TMP/UV.

 JoVE Biology

Principles of Rodent Surgery for the New Surgeon

1Charles River, Research Models and Services


JoVE 2586

Before attempting surgery, a new surgeon should have training in basic surgical techniques and concepts. This article will present basic surgical considerations with an emphasis on rodents.

 JoVE Biology

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

1Gene Expression and Signaling Research Group, Max Planck Institute for Biophysical Chemistry


JoVE 2438

Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.

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