JoVE to attend American Society for Microbiology

JoVE will be attending the 113th American Society for Microbiology General Meeting General Meeting in Denver, Colorado this month! Come visit us booth #439 in the Exhibit Hall on Sunday, May 19th, Monday, May 20th, or Tuesday, May 21st. If you’ve ever thought about publishing a video of your research methods, or have questions about how to subscribe to this unique resource, this is a great chance to speak to a member of the JoVE team in person. We will also be displaying some of our published videos, offering free trial subscriptions to JoVE, and giving away an iPad mini. We look forward to seeing you next week in Denver!

Also, make sure you follow us on twitter at @JoVEJournal and watch the official hash-tag #asm2013. Our twitter followers will have multiple opportunities throughout the conference to get special “swag bags” of JoVE gear!

Introducing JoVE Science Education!

Let’s face it, research science isn’t easy to get into. Due to a rich history and thousands of researchers adding to the knowledge pool almost daily, there is a steep learning curve that those interested in the sciences need to overcome to even get their foot in a lab’s front door. To ease the pain of meeting this threshold, JoVE, the first peer-reviewed scientific video journal, is releasing a new database of educational videos aimed at coaching new scientists on techniques they will use for the rest of their careers in research and development.

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New JoVE Articles 03/27/2013

JoVE General


Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
Article 50042 Zach Hensel1, Xiaona Fang1,2,3, Jie Xiao11Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin UniversityPublished March 15, 2013 | 467 Views

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New JoVE Articles 03/11/2013

JoVE General


Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Article 4344 Wei-Meng Woo*, Scott X. Atwood*, Hanson H. Zhen, Anthony E. OroProgram in Epithelial Biology, Stanford University School of MedicinePublished February 28, 2013 | 417 Views

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Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
Miao Zhang1, Hans R. Schöler1,2, Boris Greber11Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of MünsterPublished March 5, 2013 | 321 Views

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Article 4335

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
Article 50171 Marta Gomez-Martinez1, Debora Schmitz2, Alexander Hergovich11UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical ResearchPublished March 5, 2013 | 330 Views

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JoVE Neuroscience


Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
Christof Rickert1, Thomas Kunz1, Kerri-Lee Harris2, Paul Whitington2, Gerhard Technau11Institute of Genetics, University of Mainz, 2Department of Anatomy and Neuroscience, University of MelbournePublished March 4, 2013 | 336 Views

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Article 50150

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain
Article 50215 Ida Annunziata*, Annette Patterson*, Alessandra d’AzzoDepartment of Genetics, St Jude Children’s Research HospitalPublished March 4, 2013 | 326 Views

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JoVE Immunology and Infection


Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
Lorena Michelle Coronado1,2, Nicole Michelle Tayler1,2, Ricardo Correa1,2, Rita Marissa Giovani3, Carmenza Spadafora11Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)Published March 2, 2013 | 257 Views

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Article 50342

JoVE Clinical and Translational Medicine


A Zebrafish Model of Diabetes Mellitus and Metabolic Memory
Article 50232 Robert V. Intine1, Ansgar S. Olsen1, Michael P. Sarras Jr.21Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Chicago Medical School, Rosalind Franklin University of Medicine and SciencePublished February 28, 2013 | 425 Views

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Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling
Alexander Michael Guendel*1, Kyle S. Martin*1, Joshua Cutts2, Patricia L. Foley3, Alexander M. Bailey1, Feilim Mac Gabhann4, Trevor R. Cardinal2, Shayn M. Peirce11Department of Biomedical Engineering, University of Virginia, 2Department of Biomedical Engineering, California Polytechnic State University, 3Office of Animal Welfare, University of Virginia, 4Department of Biomedical Engineering & Institute for Computational Medicine, Johns Hopkins UniversityPublished March 3, 2013 | 250 Views

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Article 50218

JoVE Bioengineering


Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Article 50176 Ron B. Shmueli1,2, Nupura S. Bhise1,2, Jordan J. Green1,2,3,41Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of MedicinePublished March 1, 2013 | 224 Views

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Graphene Coatings for Biomedical Implants
Ramakrishna Podila1,2, Thomas Moore3, Frank Alexis3, Apparao Rao1,41Department of Physics, Clemson University, 2Department of Pharmacology and Toxicology, East Carolina University, 3Department of Bioengineering, Clemson University, 4Center for Optical Materials Science and Engineering Technologies, Clemson UniversityPublished March 1, 2013 | 317 Views

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Article 50276

JoVE Applied Physics


Determining 3D Flow Fields via Multi-camera Light Field Imaging
Article 4325 Tadd T. Truscott1, Jesse Belden2, Joseph R. Nielson1, David J. Daily1, Scott L. Thomson11Department of Mechanical Engineering, Brigham Young University, 2Naval Undersea Warfare Center, Newport, RIPublished March 6, 2013 | 322 Views

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JoVE Chemistry


Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
Pooja Sharma1,2, Mariam Kaywan-Lutfi1, Logesvaran Krshnan1,2, Eamon F. X. Byrne1,2, Melissa Joy Call1,2, Matthew Edwin Call1,21Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of MelbournePublished March 6, 2013 | 256 Views

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Article 50141

New Institutions Subscribing to JoVE! 3/24/2013

Check out some of the latest institutions to subscribe to JoVE (Journal of Visualized Experiments)

 

 

Want JoVE at your institution? Recommend JoVE to your librarian!

New Articles 3/4/2013

JoVE General


Design and Use of Multiplexed Chemostat Arrays
Article 50262 Aaron W. Miller, Corrie Befort, Emily O. Kerr, Maitreya J. DunhamDepartment of Genome Sciences, University of WashingtonPublished February 23, 2013 | 340 Views

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Serial
Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in
Culture for Derivation of Long-term Adult Mouse SSC Lines
Laura A. Martin, Marco SeandelDepartment of Surgery, Weill Cornell Medical CollegePublished February 25, 2013 | 230 Views

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Article 50017

Trajectory Data Analyses for Pedestrian Space-time Activity Study
Article 50130 Feng Qi1, Fei Du21School of Environmental and Life Sciences, Kean University, 2Department of Geography, University of Wisconsin-MadisonPublished February 25, 2013 | 305 Views

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JoVE Neuroscience


Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe
Kelsey J. R. P. Byers, Elischa Sanders, Jeffrey A. RiffellDepartment of Biology, University of WashingtonPublished February 24, 2013 | 273 Views

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Article 4381

JoVE Clinical and Translational Medicine


Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion
Article 50015 Robert W. Regenhardt1, Saeed Ansari2, Hassan Azari3, Kenneth J. Caldwell2, Adam P. Mecca11Department of Physiology and Functional Genomics, University of Florida, 2Department of Neurosurgery, McKnight Brain Institute, University of Florida, 3Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, IranPublished February 22, 2013 | 280 Views

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In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
Yimu Yang, Gaoqing Yang, Eric P. SchmidtDivision of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of MedicinePublished February 22, 2013 | 298 Views

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Article 50322

Pulmonary
Vein Isolation by Radiofrequency Ablation followed by Implantation of a
WATCHMAN Left Atrial Appendage Occlusion Device
Article 3818 Martin J. Swaans, Benno J.W.M. Rensing, Lucas V.A. BoersmaDepartment of Cardiology, St. Antonius Hospital, The NetherlandsPublished February 26, 2013 | 455 Views

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Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation
Ioanna Kosmidou1, Shannnon Wooden1, Brian Jones2, Thomas Deering1, Andrew Wickliffe1, Dan Dan11Department of Cardiac Electrophysiology, Piedmont Heart Institute, 2Cardiac Rhythm, Medtronic Inc.Published February 26, 2013 | 181 Views

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Article 50247

JoVE Bioengineering


Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination
Article 4430 Yan Liu, Jiayu LiaoDepartment of Bioengineering, Bourns College of Engineering, University of California, RiversidePublished February 21, 2013 | 234 Views

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Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
Graham Marsh, Richard E. WaughDepartment of Biomedical Engineering, University of RochesterPublished February 21, 2013 | 297 Views

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Article 50163

JoVE Applied Physics


Characterization
of Surface Modifications by White Light Interferometry: Applications in
Ion Sputtering, Laser Ablation, and Tribology Experiments
Article 50260 Sergey V. Baryshev1, Robert A. Erck2, Jerry F. Moore3, Alexander V. Zinovev1, C. Emil Tripa1, Igor V. Veryovkin11Materials Science Division, Argonne National Laboratory, 2Energy Systems Division, Argonne National Laboratory, 3MassThink LLCPublished February 27, 2013 | 192 Views

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Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition
Paolo Gondoni1, Matteo Ghidelli1, Fabio Di Fonzo1,2, Andrea Li Bassi1,2, Carlo S. Casari11Department of Energy and NEMAS – Center for NanoEngineered Materials and Surfaces, Politecnico di Milano, 2Center for Nano Science and Technology, Instituto Italiano di TecnologiaPublished February 27, 2013 | 206 Views

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Article 50297

This Week’s New Articles!

JoVE General


Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice
Article 50183 Chady H. Hakim, Nalinda B. Wasala, Dongsheng DuanDepartment of Molecular Microbiology and Immunology, School of Medicine, University of MissouriPublished February 9, 2013 | 343 Views

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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
Jun Liang1, Sheng Xiong2,3, Cathy Savage-Dunn2,31Department of Science, Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology, Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center, Queens College, The City University of New York (CUNY)Published February 13, 2013 | 149 Views

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Article 4373

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Article 4393 Dilyara Cheranova, Margaret Gibson, Suman Chaudhary, Li Qin Zhang, Daniel P. Heruth, Dmitry N. Grigoryev, Shui Qing YeChildren’s Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas CityPublished February 13, 2013 | 295 Views

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JoVE Neuroscience

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
Andrew J. Woolley1, Himanshi A. Desai1, Janak Gaire2, Andrew L. Ready1, Kevin J. Otto1,21Weldon School of Biomedical Engineering, Purdue University, 2Department of Biological Sciences, Purdue UniversityPublished February 11, 2013 | 236 Views

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Article 50126

Neonatal Subventricular Zone Electroporation
Article 50197 David M. Feliciano, Carlos A. Lafourcade, Angélique BordeyDepartment of Neurosurgery and Cellular & Molecular Physiology, Yale University School of MedicinePublished February 11, 2013 | 251 Views

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JoVE Immunology and Infection

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Gleb Pishchany, Kathryn P. Haley, Eric P. SkaarDepartment of Pathology, Microbiology and Immunology, Vanderbilt University Medical SchoolPublished February 7, 2013 | 302 Views

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Article 50072

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
Article 50157 Jennifer L. Harcourt, Lia M. HaynesNational Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC)Published February 7, 2013 | 236 Views

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JoVE Clinical and Translational Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
Rebecca E. Nakles1, Sarah L. Millman1, M. Carla Cabrera1, Peter Johnson1,2, Susette Mueller1,2, Philipp S. Hoppe3, Timm Schroeder3, Priscilla A. Furth1,2,4,51Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München – German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook UniversityPublished February 8, 2013 | 216 Views

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Article 50198

Angiogenesis in the Ischemic Rat Lung
Article 50217 John Jenkins, Elizabeth WagnerJohns Hopkins Asthma and Allergy Center, Division of Pulmonary and Critical Care Medicine, Johns Hopkins UniversityPublished February 8, 2013 | 300 Views

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Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice
Sebastián Dante Calligaris, Micaela Ricca, Paulette CongetInstituto de Ciencias, Facultad de Medicina, Clínica Alemana Universidad del DesarrolloPublished February 10, 2013 | 269 Views

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Article 50050

JoVE Bioengineering

Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering
Article 50018 Shivanjali Joshi-Barr1, Jerome V. Karpiak2, Yogesh Ner1, Jessica H. Wen3, Adam J. Engler3, Adah Almutairi1,21Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 2Biomedical Sciences Program, University of California, San Diego, 3Department of Bioengineering, University of California, San DiegoPublished February 12, 2013 | 185 Views

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JoVE Applied Physics

Bringing the Visible Universe into Focus with Robo-AO
Christoph Baranec1,2, Reed Riddle1, Nicholas M. Law3, A.N. Ramaprakash4, Shriharsh P. Tendulkar2, Khanh Bui1, Mahesh P. Burse4, Pravin Chordia4, Hillol K. Das4, Jack T.C. Davis1, Richard G. Dekany1, Mansi M. Kasliwal5, Shrinivas R. Kulkarni1,2, Timothy D. Morton2, Eran O. Ofek6, Sujit Punnadi41Caltech Optical Observatories, California Institute of Technology, 2Department of Astronomy, California Institute of Technology, 3Dunlap Institute for Astronomy and Astrophysics, University of Toronto, 4Inter-University Centre for Astronomy & Astrophysics, 5Observatories of the Carnegie Institution for Science, 6Benoziyo Center for Astrophysics, Weizmann Institute of SciencePublished February 12, 2013 | 702 Views

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Article 50021

Announcing: JoVE Chemistry

Since 2006, JoVE has brought high quality video articles to the biological sciences community. Currently, JoVE publishes high quality scientific video protocols in the areas of neuroscience, general biology, bioengineering, immunology and infection, clinical and translational medicine, and applied physics.

Today, February 4, 2013, JoVE proudly launches its 7th section: JoVE Chemistry. Following its successful introduction of video publications for the biological and physical sciences, JoVE received numerous requests for a chemistry counterpart. In response, the journal is launching a new section, JoVE Chemistry dedicated to visualized publication of experiments across different areas of chemistry research including organic chemistry, chemical biology, electrochemistry, and polymer chemistry, among others. Check out our Chemistry announcement here:

“Similar to research in biology and physics, experimental research in chemistry suffers from a lack of reproducibility that can be solved by visualized publication. Therefore, it is natural to expand JoVE‘s novel publication approach to chemistry as well”, says Moshe Pritsker, the CEO and co-founder of JoVE.

Click here to learn more about <em>JoVE</em> Chemistry