Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
|
|
07/30/2010
Amélie Bruneau, Nadia Champagne, Paule Cousineau-Pelletier, Gabriel Parent, Eve Langelier
Groupe PERSEUS, Faculté de Génie Département de génie mécanique, Université de Sherbrooke
This article describes the experimental procedures used to prepare rat tail tendons for biomechanical and mechanobiological studies. Several features of the main steps in preparation are demonstrated, beginning with extraction, cross-sectional area measurement, rinsing and loading into the bioreactor chamber.
07/29/2010
Lauren J. Buro, Shaili Shah, Melissa A. Henriksen
Department of Biology, University of Virginia
This protocol describes how chromatin immunoprecipitation (ChIP) is used to study the dynamic alterations to the chromatin template that regulate transcription induced by a signal transduction pathway.
07/28/2010
Huy Nguyen1, Cristy Loustaunau1, Alexander Facista1, Lois Ramsey1, Nadia Hassounah1, Hilary Taylor1, Robert Krouse2, 3, Claire M. Payne1, 4, V. Liana Tsikitis3, Steve Goldschmid5, Bhaskar Banerjee5, Rafael F. Perini5, Carol Bernstein1
1Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, 2Southern Arizona Veterans Affairs Health Care System, Tucson, AZ, 3Department of Surgery, College of Medicine, University of Arizona, Tucson, 4Biomedical Diagnostics and Research, Tucson, AZ, 5Department of Medicine, College of Medicine, University of Arizona, Tucson
Reduced/absent expression of Pms2 and/or ERCC1 in entire crypts is a frequent event within 10 cm on each side of colonic adenocarcinomas, likely the basis of a field defect with high mutability and progression to cancer. Deficiency in Ku86 or CcOI is much less frequent in these field defects.
07/27/2010
Colin J. Thalhofer1, Joel W. Graff2, Laurie Love-Homan3, Suzanne M. Hickerson4, Noah Craft5, Stephen M. Beverley4, Mary E. Wilson6, 7
1Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center, 2Department of Biochemistry, University of Iowa, and the VA Medical Center, 3Department of Internal Medicine, University of Iowa, 4Department of Molecular Microbiology, Washington University School of Medicine, 5Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program, Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
07/26/2010
Weihua Pan1, Ping Xin1, Susan Patrick1, Stacey Dean2, Christine Keating2, Gary Clawson3, 4
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
Read More Articles >>
Get articles delivered to you via RSS: |
|
Make a SuggestionIs there a technique you would like to see published? Click the button above to make a suggestion. |
|
|
|
 |