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Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
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Whole-cell recordings of light evoked excitatory synaptic currents in the retinal slice Birgit Werner1, Paul B. Cook2, 1, Christopher L. Passaglia3, 1
1Program in Neuroscience, Boston University, 2Department of Biology, Boston University, 3Department of Biomedical Engineering, Boston University
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| This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina. |
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Freezing, Thawing, and Packaging Cells for Transport Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory Network, Inc
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| Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks. |
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Isolation of early Hematopoietic Stem cells from Murine Yolk Sac and AGM Kelly Morgan1, Michael Kharas1, Elaine Dzierzak2, D. Gary Gilliland1, 3
1Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School, 2Department of Cell Biology and Genetics, Erasmus University Medical Center, 3Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School
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| This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells. |
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Isolation and Analysis of Hematopoietic Stem Cells from the Placenta Christos Gekas, Katrin E. Rhodes, Hanna K. A. Mikkola
Jonsson Comprehensive Cancer Center, University of California, Los Angeles
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| We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta. |
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Counting and Determining the Viability of Cultured Cells Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory Network, Inc
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| Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer. |
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