Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Visualizing the Beating Heart in <em>Drosophila</em>

ヒト表皮再構築モデルを用いたIn vitro皮膚刺激試験

利用生物流系统在受控剪切流作用下进行活细胞实验

制御されたせん断流動下における生存細胞実験のためのBioFlux System

无饲养细胞条件下进行人类诱发型多潜能干细胞的冻藏

无饲养细胞条件下进行人类诱发型多潜能干细胞的调适和传代

相比肿瘤细胞皮下注射或腹腔注射的异种移植模型,同位动物模型的主要优势在于它能更好的表现人类癌症,所以也能使我们更好的了解人类癌症及其对治疗的反应.

纳微量2000c 分光光度计- 微量体积蛋白浓度的测定

运用自动细胞计数器简化基因表达研究:利用siRNA抑制Namalwa细胞中依靠IL-4基因的表达

<em>Cancer Borealis</em> Stomatogastric Nervous System Dissection

<em>Drosophila</em> Larval NMJ Immunohistochemistry

<em>Homarus Americanus</em> Stomatogastric Nervous System Dissection

<em>In situ</em> Quantification of Pancreatic Beta-cell Mass in Mice

<em>In situ</em> Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

<em>In utero</em> and <em>ex vivo</em> Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

<em>In vivo</em> Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

<em>In vivo</em> Ca<sup>2+</sup>- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>In vivo</em> Imaging of Deep Cortical Layers using a Microprism

<em>In vivo</em> Imaging of Transgenic <em>Leishmania</em> Parasites in a Live Host

<em>In vivo</em>-like Organotypic Murine Retinal Wholemount Culture

<em>In-vivo</em> Centrifugation of <em>Drosophila</em> Embryos

<em>In-vivo</em> Detection of Protein-protein Interactions on Micro-patterned Surfaces

<em>Toxoplasma gondii</em> Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions

A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities

A Cell-to-cell Macromolecular Transport Assay <em>in Planta</em> Utilizing Biolistic Bombardment

A Chromatin Assay for Human Brain Tissue

A Craniotomy Surgery Procedure for Chronic Brain Imaging

A Gradient-generating Microfluidic Device for Cell Biology

A High-Throughput Method For Zebrafish Sperm Cryopreservation and <em>In Vitro</em> Fertilization

A high-throughput method to globally study the organelle morphology in S. cerevisiae

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

A New High Sensitivity Tandem Quadrupole Mass Spectrometer for Quantitative LC/MS/MS Analysis of Low Exposure Pharmaceuticals

A Novel Approach for Documenting Phosphenes Induced by Transcranial Magnetic Stimulation

A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in <em>Drosophila</em>

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells <em>In Vivo</em>

A Protocol for the Production of KLRG1 Tetramer

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

A Simple Composite Phenotype Scoring System for Evaluating Mouse Models of Cerebellar Ataxia

A Tactile Automated Passive-Finger Stimulator (TAPS)

A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells <em>In situ</em>

A Video Demonstration of Preserved Piloting by Scent Tracking but Impaired Dead Reckoning After Fimbria-Fornix Lesions in the Rat

AC Electrokinetic Phenomena Generated by Microelectrode Structures

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

ALS - Motor Neuron Disease: Mechanism and Development of New Therapies

Ambulatory ECG Recording in Mice

An <em>In vitro</em> FluoroBlok Tumor Invasion Assay

An <em>In Vitro</em> Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)

An Improved Method of RNA Isolation from Loblolly Pine (<em>P. taeda</em> L.) and Other Conifer Species

An Interview with Nobel Laureate Douglas Osheroff, Physics 1996

An interview with Nobel laureate Roy Glauber, Physics 2005

An Interview with Nobel Laureate Theodor Hansch, Physics 2005

Analysis of Gene Expression in Emerald Ash Borer (<em>Agrilus planipennis</em>) Using Quantitative Real Time-PCR

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

Anterior Cervical Discectomy and Fusion in the Ovine Model

Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Aortic Ring Assay

Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson's Disease Genes

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

Applying Microfluidics to Electrophysiology

Assay for Adhesion and Agar Invasion in S. cerevisiae

Assay for Neural Induction in the Chick Embryo

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using <sup>31</sup>P NMR Spectroscopy

Assessment of Ultrasonic Vocalizations During Drug Self-administration in Rats

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Bacterial Gene Expression Analysis Using Microarrays

Basics of Multivariate Analysis in Neuroimaging Data

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

BIOCIUS - RapidFire System

Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock

Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees

BioFlux System for Live Cell Experiments Under Controlled Shear Flow - ADVERTISEMENT

Biology of Microbial Communities - Interview

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

BioMEMS and Cellular Biology: Perspectives and Applications

BioMEMS: Forging New Collaborations Between Biologists and Engineers

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Brain Banking: Making the Most of your Research Specimens

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes

Calcium Imaging of Cortical Neurons using Fura-2 AM

Catheterization of Intestinal Loops in Ruminants

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

Cell Capture Using a Microfluidic Device

Cell Electrofusion Visualized with Fluorescence Microscopy

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay

Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at <em>Drosophila</em> Neuromuscular Junctions

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Chick <em>ex ovo</em> Culture and <em>ex ovo</em> CAM Assay: How it Really Works

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects

Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Chronic <em>Salmonella</em> Infected Mouse Model

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

Computer-Generated Animal Model Stimuli

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

CoolCell - Controlled Cell Freezing w/o Alcohol or Maintenance Cost - ADVERTISEMENT

Cortical Neurogenesis: Transitioning from Advances in the Laboratory to Cell-Based Therapies

Counting and Determining the Viability of Cultured Cells

Counting Human Neural Stem Cells

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Culture and Maintenance of Human Embryonic Stem Cells

Culture of Mouse Neural Stem Cell Precursors

Culture of myeloid dendritic cells from bone marrow precursors

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing <em>in ovo</em> Electroporation

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Demonstration of <em>Cutaneous Allodynia</em> in Association with Chronic Pelvic Pain

Denaturing Gradient Gel Electrophoresis (DGGE)

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

Dendra2 Photoswitching through the Mammary Imaging Window

Depletion of Specific Cell Populations by Complement Depletion

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Human Embryonic Stem Cells by Immunosurgery

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Detection of Protein Ubiquitination

Determining heat and mechanical pain threshold in inflamed skin of human subjects

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Development of automated imaging and analysis for zebrafish chemical screens.

Development of New Therapeutic Applications Using Microfluidics

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Digital Microfluidics for Automated Proteomic Processing

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Direct Tracheal Instillation of Solutes into Mouse Lung

Dissecting and Recording from The C. Elegans Neuromuscular Junction

Dissecting the Non-human Primate Brain in Stereotaxic Space

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Dissection of <em>Saccharomyces Cerevisiae</em> Asci

Dissection of 6.5 dpc Mouse Embryos

Dissection of Drosophila Ovaries

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Dissection of Larval CNS in Drosophila Melanogaster

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

Dissection of Oenocytes from Adult <em>Drosophila melanogaster</em>

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

Dissection of Organs from the Adult Zebrafish

DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation

DNA Stable-Isotope Probing (DNA-SIP)

DNA Transfection of Mammalian Skeletal Muscles using <em>In Vivo</em> Electroporation

DNA-based Fish Species Identification Protocol

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Double Fluorescence <em>in situ</em> Hybridization in Fresh Brain Sections

Double Whole Mount in situ Hybridization of Early Chick Embryos

Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture

Drosophila Larval NMJ Dissection

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices

Electrochemotherapy of Tumours

Electrode Fabrication and Implantation in <em>Aplysia californica</em> for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

Electron Cryotomography of Bacterial Cells

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Electrophoretic Separation of Proteins

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

Electrophysiological Recording in the <em>Drosophila</em> Embryo

Electroporation of Mycobacteria

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

Elevated Plus Maze for Mice

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia

Engineering Cell-permeable Protein

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

Erratum: Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons.

ES Cell-derived Neuroepithelial Cell Cultures

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Eukaryotic Polyribosome Profile Analysis

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

Ex vivo Mechanical Loading of Tendon

Experimental Approaches to Tissue Engineering

Experimental Metastasis Assay

Experion RNA Assay - Bio-Rad - ADVERTISEMENT

Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy

Expression of Recombinant Proteins in the Methylotrophic Yeast <em>Pichia pastoris</em>

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Extraction of the EPP Component from the Surface EMG

Eye Movement Monitoring of Memory

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

Fabrication of Amperometric Electrodes

Fabrication of Myogenic Engineered Tissue Constructs

Fabrication of the Thermoplastic Microfluidic Channels

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Feeder非存在下でのヒトiPS細胞の凍結保存

Feeder非存在下でのヒトiPS細胞の凍結保存

Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Floral-dip Transformation of <em>Arabidopsis thaliana</em> to Examine <em>pTSO2::β-glucuronidase</em> Reporter Gene Expression

Fluorescence <em>in situ</em> hybridization (FISH) Protocol in Human Sperm

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescent Labeling of <em>Drosophila</em> Heart Structures

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Focal Ca<sup>2+</sup> Transient Detection in Smooth Muscle

Freezing and Thawing Human Embryonic Stem Cells

Freezing Human ES Cells

Freezing, Thawing, and Packaging Cells for Transport

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

Functional Imaging with Reinforcement, Eyetracking, and Physiological Monitoring

Functional Mapping with Simultaneous MEG and EEG

Gene-gun Transfection of Hippocampal Neurons

Generating Chimeric Zebrafish Embryos by Transplantation

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Generation of Human CD40-activated B cells

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Single-Cell Suspensions from Mouse Neural Tissue

Generation of Stable Transgenic C. elegans Using Microinjection

Generation of Transgenic <em>C. elegans</em> by Biolistic Transformation

Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (<em>Danio rerio</em>)

Gross Dissection of the Stomach of the Lobster, <em>Homarus Americanus</em>

Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

Harvesting and Preparing <em>Drosophila</em> Embryos for Electrophysiological Recording and Other Procedures

Harvesting Sperm and Artificial Insemination of Mice

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

Heterotopic Heart Transplantation in Mice

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

High Content Screeningによるアストロサイトと神経細胞の毒性アッセイ

High Density Event-related Potential Data Acquisition in Cognitive Neuroscience

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Homemade Site Directed Mutagenesis of Whole Plasmids

Homing of Hematopoietic Cells to the Bone Marrow

Horizontal Slice Preparation of the Retina

How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)

Human ES cells: Starting Culture from Frozen Cells

Human Fear Conditioning Conducted in Full Immersion 3-Dimensional Virtual Reality

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Human T Lymphocyte Isolation, Culture and Analysis of Migration <em>In Vitro</em>

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model

Imaging Mismatch Repair and Cellular Responses to DNA Damage in <em>Bacillus subtilis</em>

Immunoblot Analysis

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

Immunocytochemistry: Human Neural Stem Cells

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

Implantation of Engineered Tissue in the Rat Heart

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

In Ovo Electroporations of HH Stage 10 Chicken Embryos

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

In situ Protocol for Butterfly Pupal Wings Using Riboprobes

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes

Injection of dsRNA into Female A. aegypti Mosquitos

Interview with Edward Kravitz

Interview with Eric R. Kandel: From Memory, Free Will, and the Problem with Freud to Fortunate Decisions

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation

Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase

Interview: Protein Folding and Studies of Neurodegenerative Diseases

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Intraperitoneal Injection into Adult Zebrafish

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Intravitreous Injection for Establishing Ocular Diseases Model

Investigating the Immunological Mechanisms Underlying Organ Transplant Rejection

Investigating the Microbial Community in the Termite Hindgut - Interview

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in <em>Arabidopsis thaliana</em>

Isolating Stem Cells from Soft Musculoskeletal Tissues

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Isolation and Purification of <em>Drosophila</em> Peripheral Neurons by Magnetic Bead Sorting

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

Isolation of Genomic DNA from Mouse Tails

Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

Isolation of Labile Multi-protein Complexes by <em>in vivo</em> Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

Isolation of Mouse Peritoneal Cavity Cells

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of <em>Arabidopsis thaliana</em>

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Issue 9: Transplantation, Electroporation, and more...

JoVE - Video from Paulo

JoVE 10th Issue

Jove 3rd Issue

JoVE 4th Issue

JoVE 5th Issue: Mosquito Biology

JoVE 6th Issue

JoVE 7th Issue: Bioengineering and Immunology

JoVE Issue 8: Microfluidics, cell patterning and more.

Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain

Labeling and Imaging Cells in the Zebrafish Hindbrain

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

LAD-Ligation: A Murine Model of Myocardial Infarction

Laparoscopic Left Liver Sectoriectomy of Caroli's Disease Limited to Segment II and III

Large Insert Environmental Genomic Library Production

Large Volume (20L+) Filtration of Coastal Seawater Samples

Large-Scale Screens of Metagenomic Libraries

Laser Capture Microdissection of <em>Drosophila</em> Peripheral Neurons

Laser Capture Microdissection of Mammalian Tissue

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the <em>Drosophila</em> Wing Disc

Laser-Induced Chronic Ocular Hypertension Model on SD Rats

Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lentivirus Production

Light/dark Transition Test for Mice

Linearization of the Bradford Protein Assay

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Dissection of <em>Drosophila</em> Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

Live Imaging Of <em>Drosophila melanogaster </em>Embryonic Hemocyte Migrations

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in <em>Xenopus laevis</em>

Loading Drosophila Nerve Terminals with Calcium Indicators

Localized RNAi and Ectopic Gene Expression in the Medicinal Leech

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Low-stress Route Learning Using the Lashley III Maze in Mice

Magnetic Resonance Spectroscopy of live <em>Drosophila melanogaster</em> using Magic Angle Spinning

Maintaining Wolbachia in Cell-free Medium

Major Components of the Light Microscope

Making Gynogenetic Diploid Zebrafish by Early Pressure

Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

MALDI Sample Preparation: the Ultra Thin Layer Method

Mammary Epithelial Transplant Procedure

Mating and Tetrad Separation of <em>Chlamydomonas reinhardtii</em> for Genetic Analysis

Measurement Of Neuromagnetic Brain Function In Pre-school Children With Custom Sized MEG

Measures of Heart and Ventilatory Rates in Freely Moving Crayfish

Measuring <em>Caenorhabditis elegans</em> Life Span on Solid Media

Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Measuring Exocytosis in Neurons Using FM Labeling

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

Measuring Replicative Life Span in the Budding Yeast

Measuring the 50% Haemolytic Complement (CH<sub>50</sub>) Activity of Serum

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mesoscopic Fluorescence Tomography for <em>In-vivo</em> Imaging of Developing <em>Drosophila</em>

Method for Culture of Early Chick Embryos ex vivo (New Culture)

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Method for the Isolation of <em>Francisella tularensis</em> Outer Membranes

Method for Whole Mount Antibody Staining in Chick

Methods for Patch Clamp Capacitance Recordings from the Calyx

Methods for the Study of the Zebrafish Maxillary Barbel

Methylated DNA Immunoprecipitation

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

Micro-drive Array for Chronic <em>in vivo</em> Recording: Drive Fabrication

Micro-drive Array for Chronic <em>in vivo</em> Recording: Tetrode Assembly

Micro-scale Engineering for Cell Biology

Microbial Communities in Nature and Laboratory - Interview

Microcontact Printing of Proteins for Cell Biology

Microdissection of Zebrafish Embryonic Eye Tissues

Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Microfluidic Applications for Disposable Diagnostics

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Microinjection of Xenopus Laevis Oocytes

Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection Techniques for Studying Mitosis in the <em>Drosophila melanogaster</em> Syncytial Embryo

Microsurgical Clip Obliteration of Middle Cerebral Aneurysm Using Intraoperative Flow Assessment

Microvolume Protein Concentration Determination Using the NanoDrop Spectrophotometer

MISSION esiRNA for RNAi Screening in Mammalian Cells

Molecular Evolution of the Tre Recombinase

Monitoring Actin Disassembly with Time-lapse Microscopy

Monitoring Acupuncture Effects on Human Brain by fMRI

Monitoring Heart Function in Larval <em>Drosophila melanogaster</em> for Physiological Studies

Monitoring Plant Hormones During Stress Responses

Morris Water Maze Experiment

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Mouse Adrenal Chromaffin Cell Isolation

Mouse Dorsal Forebrain Explant Isolation

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

Mouse Models of Periventricular Leukomalacia

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

Multielectrode Array Recordings of the Vomeronasal Epithelium

Murine Bioluminescent Hepatic Tumour Model

Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)

Murine echocardiography and ultrasound imaging

Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test

Murine Model of CD40-activation of B cells

Murine Model of Hindlimb Ischemia

Murine Pancreatic Islet Isolation

Murine Renal Transplantation Procedure

Murine Skin Transplantation

NanoDrop 2000c分光光度計 微量タンパク濃度定量

Neurocircuit Assays for Seizures in Epilepsy Mutants of <em>Drosophila</em>

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Neutrophil Isolation Protocol

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices

Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Noninvasive <em>In Vivo</em> Small Animal MRI and MRS: Basic Experimental Procedures

Novel Apparatus and Method for Drug Reinforcement

Nuclear Transfer into Mouse Oocytes

Obtaining Eggs from Xenopus laevis Females

Obtaining Hemocytes from the Hawaiian Bobtail Squid <em>Euprymna scolopes</em> and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Obtaining Highly Purified <em>Toxoplasma gondii</em> Oocysts by a Discontinuous Cesium Chloride Gradient

Ole Isacson: Development of New Therapies for Parkinson's Disease

Olfactory Behavioral Testing in the Adult Mouse

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Operant Learning of Drosophila at the Torque Meter

Optical Mapping of Langendorff-perfused Rat Hearts

Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Organotypic Culture of Adult Rabbit Retina

Organotypic Slice Culture of E18 Rat Brains

Orthotopic Hind-Limb Transplantation in Rats

Orthotopic Mouse Model of Colorectal Cancer

Osmotic Avoidance in <em>Caenorhabditis elegans</em>: Synaptic Function of Two Genes, Orthologues of Human <em>NRXN1</em> and <em>NLGN1</em>, as Candidates for Autism

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin