Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
Visualizing the Beating Heart in <em>Drosophila</em>
ヒト表皮再構築モデルを用いたIn vitro皮膚刺激試験
利用生物流系统在受控剪切流作用下进行活细胞实验
制御されたせん断流動下における生存細胞実験のためのBioFlux System
纳微量2000c 分光光度计- 微量体积蛋白浓度的测定
<em>Cancer Borealis</em> Stomatogastric Nervous System Dissection
<em>Drosophila</em> Larval NMJ Immunohistochemistry
<em>Homarus Americanus</em> Stomatogastric Nervous System Dissection
<em>In utero</em> and <em>ex vivo</em> Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
<em>In vivo</em> Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
<em>In vivo</em> Ca<sup>2+</sup>- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
<em>In vivo</em> Imaging of Deep Cortical Layers using a Microprism
<em>In vivo</em>-like Organotypic Murine Retinal Wholemount Culture
A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
A Chromatin Assay for Human Brain Tissue
A Craniotomy Surgery Procedure for Chronic Brain Imaging
A Gradient-generating Microfluidic Device for Cell Biology
A High-Throughput Method For Zebrafish Sperm Cryopreservation and <em>In Vitro</em> Fertilization
A high-throughput method to globally study the organelle morphology in S. cerevisiae
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila
A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window
A Method For Production of Recombinant mCD1d Protein in Insect Cells.
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in <em>Drosophila</em>
A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells <em>In Vivo</em>
A Protocol for the Production of KLRG1 Tetramer
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
A Tactile Automated Passive-Finger Stimulator (TAPS)
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells <em>In situ</em>
A Video Demonstration of Preserved Piloting by Scent Tracking but Impaired Dead Reckoning After Fimbria-Fornix Lesions in the Rat
AC Electrokinetic Phenomena Generated by Microelectrode Structures
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
ALS - Motor Neuron Disease: Mechanism and Development of New Therapies
An <em>In vitro</em> FluoroBlok Tumor Invasion Assay
An <em>In Vitro</em> Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)
An Improved Method of RNA Isolation from Loblolly Pine (<em>P. taeda</em> L.) and Other Conifer Species
An Interview with Nobel Laureate Douglas Osheroff, Physics 1996
An interview with Nobel laureate Roy Glauber, Physics 2005
An Interview with Nobel Laureate Theodor Hansch, Physics 2005
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
Anterior Cervical Discectomy and Fusion in the Ovine Model
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
Aortic Ring Assay
Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson's Disease Genes
Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
Applying Microfluidics to Electrophysiology
Assay for Adhesion and Agar Invasion in S. cerevisiae
Assay for Neural Induction in the Chick Embryo
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Bacterial Gene Expression Analysis Using Microarrays
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees
BioFlux System for Live Cell Experiments Under Controlled Shear Flow - Advertisement
Biology of Microbial Communities - Interview
Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
BioMEMS and Cellular Biology: Perspectives and Applications
BioMEMS: Forging New Collaborations Between Biologists and Engineers
Blood Collection from the American Horseshoe Crab, Limulus Polyphemus
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
Brain Banking: Making the Most of your Research Specimens
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
Calcium Imaging of Cortical Neurons using Fura-2 AM
Catheterization of Intestinal Loops in Ruminants
CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
Cell Capture Using a Microfluidic Device
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay
Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at <em>Drosophila</em> Neuromuscular Junctions
Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers
Chick <em>ex ovo</em> Culture and <em>ex ovo</em> CAM Assay: How it Really Works
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects
Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Computer-Generated Animal Model Stimuli
Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.
CoolCell - Controlled Cell Freezing w/o Alcohol or Maintenance Cost - Advertisement
Cortical Neurogenesis: Transitioning from Advances in the Laboratory to Cell-Based Therapies
Counting and Determining the Viability of Cultured Cells
Counting Human Neural Stem Cells
Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
Culture and Maintenance of Human Embryonic Stem Cells
Culture of Mouse Neural Stem Cell Precursors
Culture of myeloid dendritic cells from bone marrow precursors
Demonstration of <em>Cutaneous Allodynia</em> in Association with Chronic Pelvic Pain
Denaturing Gradient Gel Electrophoresis (DGGE)
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Dendra2 Photoswitching through the Mammary Imaging Window
Depletion of Specific Cell Populations by Complement Depletion
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Derivation of Human Embryonic Stem Cells by Immunosurgery
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
Detection of Protein Ubiquitination
Determining heat and mechanical pain threshold in inflamed skin of human subjects
Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
Development of New Therapeutic Applications Using Microfluidics
Digital Microfluidics for Automated Proteomic Processing
Dissecting and Recording from The C. Elegans Neuromuscular Junction
Dissecting the Non-human Primate Brain in Stereotaxic Space
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
Dissection of <em>Saccharomyces Cerevisiae</em> Asci
Dissection of 6.5 dpc Mouse Embryos
Dissection of Drosophila Ovaries
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae
Dissection of Larval CNS in Drosophila Melanogaster
Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay
Dissection of Organs from the Adult Zebrafish
DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
DNA Transfection of Mammalian Skeletal Muscles using <em>In Vivo</em> Electroporation
Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
Double Whole Mount in situ Hybridization of Early Chick Embryos
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
Drosophila Larval NMJ Dissection
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
Electrochemotherapy of Tumours
Electrophoretic Separation of Proteins
Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
Electrophysiological Recording in the <em>Drosophila</em> Embryo
Electroporation of Mycobacteria
Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
Elevated Plus Maze for Mice
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia
Engineering Cell-permeable Protein
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
Erratum: Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons.
ES Cell-derived Neuroepithelial Cell Cultures
Ex vivo Mechanical Loading of Tendon
Experimental Approaches to Tissue Engineering
Experion RNA Assay - Bio-Rad - Advertisement
Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy
Expression of Recombinant Proteins in the Methylotrophic Yeast <em>Pichia pastoris</em>
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Extraction of the EPP Component from the Surface EMG
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
Fabrication of Amperometric Electrodes
Fabrication of Myogenic Engineered Tissue Constructs
Fabrication of the Thermoplastic Microfluidic Channels
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
Flash Freezing and Cryosectioning E12.5 Mouse Brain
Fluorescence <em>in situ</em> hybridization (FISH) Protocol in Human Sperm
Fluorescence Activated Cell Sorting of Plant Protoplasts
Fluorescent Labeling of <em>Drosophila</em> Heart Structures
Focal Ca<sup>2+</sup> Transient Detection in Smooth Muscle
Freezing and Thawing Human Embryonic Stem Cells
Freezing Human ES Cells
Freezing, Thawing, and Packaging Cells for Transport
From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs
Functional Imaging with Reinforcement, Eyetracking, and Physiological Monitoring
Gene-gun Transfection of Hippocampal Neurons
Generating Chimeric Zebrafish Embryos by Transplantation
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Generation of Human CD40-activated B cells
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Generation of Stable Transgenic C. elegans Using Microinjection
Gibberella zeae Ascospore Production and Collection for Microarray Experiments.
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (<em>Danio rerio</em>)
Gross Dissection of the Stomach of the Lobster, <em>Homarus Americanus</em>
Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
Harvesting and Preparing <em>Drosophila</em> Embryos for Electrophysiological Recording and Other Procedures
Harvesting Sperm and Artificial Insemination of Mice
Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
Heterotopic Heart Transplantation in Mice
High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
Homemade Site Directed Mutagenesis of Whole Plasmids
Homing of Hematopoietic Cells to the Bone Marrow
Horizontal Slice Preparation of the Retina
Human ES cells: Starting Culture from Frozen Cells
Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
Identification of protein complexes with quantitative proteomics in S. cerevisiae
Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model
Imaging Mismatch Repair and Cellular Responses to DNA Damage in <em>Bacillus subtilis</em>
Immunoblot Analysis
Immunocytochemistry: Human Neural Stem Cells
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
Implantation of Engineered Tissue in the Rat Heart
In Ovo Electroporations of HH Stage 10 Chicken Embryos
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy
Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis
Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
Injection of dsRNA into Female A. aegypti Mosquitos
Interview with Edward Kravitz
Interview with Eric R. Kandel: From Memory, Free Will, and the Problem with Freud to Fortunate Decisions
Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research
Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
Interview: Protein Folding and Studies of Neurodegenerative Diseases
Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
Intravitreous Injection for Establishing Ocular Diseases Model
Investigating the Immunological Mechanisms Underlying Organ Transplant Rejection
Investigating the Microbial Community in the Termite Hindgut - Interview
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
Isolation and Derivation of Mouse Embryonic Germinal Cells
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Isolation and Purification of <em>Drosophila</em> Peripheral Neurons by Magnetic Bead Sorting
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
Isolation of Genomic DNA from Mouse Tails
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
Isolation of Labile Multi-protein Complexes by <em>in vivo</em> Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
Isolation of Mouse Peritoneal Cavity Cells
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of <em>Arabidopsis thaliana</em>
Issue 9: Transplantation, Electroporation, and more...
JoVE - Video from Paulo
JoVE 10th Issue
Jove 3rd Issue
JoVE 4th Issue
JoVE 5th Issue: Mosquito Biology
JoVE 6th Issue
JoVE 7th Issue: Bioengineering and Immunology
JoVE Issue 8: Microfluidics, cell patterning and more.
Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
LAD-Ligation: A Murine Model of Myocardial Infarction
Laparoscopic Left Liver Sectoriectomy of Caroli's Disease Limited to Segment II and III
Large Insert Environmental Genomic Library Production
Large Volume (20L+) Filtration of Coastal Seawater Samples
Large-Scale Screens of Metagenomic Libraries
Laser Capture Microdissection of Mammalian Tissue
Laser-Induced Chronic Ocular Hypertension Model on SD Rats
Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community
Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
Lentivirus Production
Light/dark Transition Test for Mice
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Live Dissection of <em>Drosophila</em> Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining
Live Imaging Of <em>Drosophila melanogaster </em>Embryonic Hemocyte Migrations
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
Loading Drosophila Nerve Terminals with Calcium Indicators
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
Maintaining Wolbachia in Cell-free Medium
Major Components of the Light Microscope
Making Gynogenetic Diploid Zebrafish by Early Pressure
Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
MALDI Sample Preparation: the Ultra Thin Layer Method
Mating and Tetrad Separation of <em>Chlamydomonas reinhardtii</em> for Genetic Analysis
Measurement Of Neuromagnetic Brain Function In Pre-school Children With Custom Sized MEG
Measures of Heart and Ventilatory Rates in Freely Moving Crayfish
Measuring <em>Caenorhabditis elegans</em> Life Span on Solid Media
Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
Measuring Exocytosis in Neurons Using FM Labeling
Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
Measuring Replicative Life Span in the Budding Yeast
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
Mesoscopic Fluorescence Tomography for <em>In-vivo</em> Imaging of Developing <em>Drosophila</em>
Method for Culture of Early Chick Embryos ex vivo (New Culture)
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
Method for Whole Mount Antibody Staining in Chick
Methods for Patch Clamp Capacitance Recordings from the Calyx
Methods for the Study of the Zebrafish Maxillary Barbel
Methylated DNA Immunoprecipitation
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
Micro-drive Array for Chronic <em>in vivo</em> Recording: Drive Fabrication
Micro-drive Array for Chronic <em>in vivo</em> Recording: Tetrode Assembly
Micro-scale Engineering for Cell Biology
Microbial Communities in Nature and Laboratory - Interview
Microcontact Printing of Proteins for Cell Biology
Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
Microfluidic Applications for Disposable Diagnostics
Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
Microinjection of Xenopus Laevis Oocytes
Microinjection of Zebrafish Embryos to Analyze Gene Function
Microinjection Techniques for Studying Mitosis in the <em>Drosophila melanogaster</em> Syncytial Embryo
Microsurgical Clip Obliteration of Middle Cerebral Aneurysm Using Intraoperative Flow Assessment
Microvolume Protein Concentration Determination Using the NanoDrop Spectrophotometer
Molecular Evolution of the Tre Recombinase
Monitoring Actin Disassembly with Time-lapse Microscopy
Monitoring Heart Function in Larval <em>Drosophila melanogaster</em> for Physiological Studies
Monitoring Plant Hormones During Stress Responses
Morris Water Maze Experiment
Mouse Adrenal Chromaffin Cell Isolation
Mouse Dorsal Forebrain Explant Isolation
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
Multielectrode Array Recordings of the Vomeronasal Epithelium
Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test
Murine Model of CD40-activation of B cells
Murine Model of Hindlimb Ischemia
Murine Pancreatic Islet Isolation
Murine Renal Transplantation Procedure
Murine Skin Transplantation
Neurocircuit Assays for Seizures in Epilepsy Mutants of <em>Drosophila</em>
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
Neutrophil Extracellular Traps: How to Generate and Visualize Them
Neutrophil Isolation Protocol
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Noninvasive <em>In Vivo</em> Small Animal MRI and MRS: Basic Experimental Procedures
Nuclear Transfer into Mouse Oocytes
Obtaining Eggs from Xenopus laevis Females
Obtaining Hemocytes from the Hawaiian Bobtail Squid <em>Euprymna scolopes</em> and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
Obtaining Highly Purified <em>Toxoplasma gondii</em> Oocysts by a Discontinuous Cesium Chloride Gradient
Ole Isacson: Development of New Therapies for Parkinson's Disease
Olfactory Behavioral Testing in the Adult Mouse
Operant Learning of Drosophila at the Torque Meter
Optical Mapping of Langendorff-perfused Rat Hearts
Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
Organotypic Culture of Adult Rabbit Retina
Organotypic Slice Culture of E18 Rat Brains
Orthotopic Mouse Model of Colorectal Cancer
Osmotic Avoidance in <em>Caenorhabditis elegans</em>: Synaptic Function of Two Genes, Orthologues of Human <em>NRXN1</em> and <em>NLGN1</em>, as Candidates for Autism
Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants
Passaging HuES Human Embryonic Stem Cell-lines with Trypsin
Passaging Human Neural Stem Cells
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes
Patterning of Embryonic Stem Cells Using the Bio Flip Chip
PDMS Device Fabrication and Surface Modification
Perspectives on Neuroscience
Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
Plastic Embedding and Sectioning of Xenopus laevis Embryos
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive
Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
Preparation and Fractionation of Xenopus laevis Egg Extracts
Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies
Preparation of <em>Aplysia</em> Sensory-motor Neuronal Cell Cultures
Preparation of 2-dGuo-Treated Thymus Organ Cultures
Preparation of Artificial Bilayers for Electrophysiology Experiments
Preparation of Dissociated Mouse Cortical Neuron Cultures
Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
Preparation of embryos for Electron Microscopy of the <em>Drosophila</em> embryonic heart tube
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
Preparing T Cell Growth Factor from Rat Splenocytes
Pressure-polishing Pipettes for Improved Patch-clamp Recording
Presynaptically Silent Synapses Studied with Light Microscopy
Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Primary Culture of Adult Rat Heart Myocytes
Primary Culture of Hippocampal Neurons from P0 Newborn Rats
Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Principles of Site-Specific Recombinase (SSR) Technology
Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
Proboscis Extension Response (PER) Assay in Drosophila
Processing the Loblolly Pine PtGen2 cDNA Microarray
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Propagation of Human Embryonic Stem (ES) Cells
Proper Care and Cleaning of the Microscope
Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Protocol for Mosquito Rearing (A. gambiae)
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus
Psychophysiological Stress Assessment Using Biofeedback
PuraMatrix Encapsulation of Cancer Cells
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Purification of Mitochondria from Yeast Cells
Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Pyrosequencing: A Simple Method for Accurate Genotyping
Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
Quantitative Phosphoproteomics in Fatty Acid Stimulated <em>Saccharomyces cerevisiae</em>
Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
Reaggregate Thymus Cultures
Recordings of Neural Circuit Activation in Freely Behaving Animals
Regulatory T cells: Therapeutic Potential for Treating Transplant Rejection and Type I Diabetes
Retrieval of Mouse Oocytes
Retro-orbital Injection in Adult Zebrafish
Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus
Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
Seawater Sampling and Collection
Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Semi-automated Optical Heartbeat Analysis of Small Hearts
Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies
Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
Silicon Microchips for Manipulating Cell-cell Interaction
Single Cell Electroporation in vivo within the Intact Developing Brain
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
Single Sensillum Recordings in the Insects <em>Drosophila melanogaster</em> and <em>Anopheles gambiae</em>
Single-cell Suction Recordings from Mouse Cone Photoreceptors
Small Bowel Transplantation In Mice
Small Volume (1-3L) Filtration of Coastal Seawater Samples
Spinal Cord Electrophysiology
Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Staining Proteins in Gels
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
Studying Aggression in Drosophila (fruit flies)
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct
Survivable Stereotaxic Surgery in Rodents
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment
Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
The 2008 Lindau Nobel Laureate Meeting: Robert Huber, Chemistry 1988
The 2009 Lindau Nobel Laureate Meeting: Aaron Ciechanover, Chemistry 2004
The 2009 Lindau Nobel Laureate Meeting: Erwin Neher, Physiology or Medicine 1991
The 2009 Lindau Nobel Laureate Meeting: Martin Chalfie, Chemistry 2008
The 2009 Lindau Nobel Laureate Meeting: Peter Agre, Chemistry 2003
The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008
The 2009 Lindau Nobel Laureate Meeting: Werner Arber, Physiology or Medicine 1978
The Gateway to the Brain: Dissecting the Primate Eye
The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia
The Ladder Rung Walking Task: A Scoring System and its Practical Application.
The Microfluidic Probe: Operation and Use for Localized Surface Processing
The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (French)
The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (German)
The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (Spanish)