Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

doi:10.3791/888

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Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Wendy Sparks, Huarong Li, Bryony Bonning
Department of Entomology, Iowa State University


			Abstract

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be infected with polyhedra by droplet feeding and diet plug-based bioassays. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents, including discussion of the pros and cons for use of baculoviruses as insecticides, and progress made in genetic enhancement of baculoviruses for improved insecticidal efficacy.


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			Protocol

Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the oral infection techniques used in this assay.


			Cite this Article

Sparks W, Li H, Bonning B (2008). Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus. JoVE. 19. http://www.jove.com/index/details.stp?id=888, doi: 10.3791/888


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10/15/2009 11:34:38 AM

Anonymous responded with a statement of type: Agree

thanks a lot!

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Keywords

Plant Biology, Issue 19, Springer Protocols, Baculovirus insecticides, recombinant baculovirus, insect pest management

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09/03/2008

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13280

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doi: 10.3791/888

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About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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