Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

doi:10.3791/889

Journal of Visualized Experiments

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Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Huarong Li, Wendy Sparks, Bryony Bonning
Department of Entomology, Iowa State University


			Abstract

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.


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			Protocol

Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.


			Cite this Article

Li H, Sparks W, Bonning B (2008). Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. JoVE. 18. http://www.jove.com/index/details.stp?id=889, doi: 10.3791/889


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12/19/2008 8:54:32 AM

Maria V. Miranda responded with a statement of type: Neutral

I would like to know who sells the syringe-aplicator like you show in this video

Thank you

MV Miranda

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01/29/2009 10:42:17 AM

Wendy Sparks responded with a statement of type: Neutral

The one used in these protocols was a Burkard Microprocessor Controller Pax-100. I hope that will help you in your search.

W. O. Sparks

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http://www.jove.com/index/details.stp?id=889

This article is:

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Keywords

Plant Biology, Issue 18, Springer Protocols, Baculovirus insecticides, recombinant baculovirus, insect pest management

Info

08/23/2008

Publish Date

12259

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doi: 10.3791/889

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About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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