Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
Huarong Li, Wendy Sparks, Bryony Bonning
Department of Entomology, Iowa State University
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0:00 Title0 2:06 Introduction126 2:51 Microapplicator-Assisted Bioassay Using Budded Virus171 7:38 Microapplicator-Assisted Bioassays Using Polyhedra458 9:30 Conclusion570
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Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.
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Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.
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Li H, Sparks W, Bonning B (2008). Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. JoVE. 18. http://www.jove.com/index/details.stp?id=889, doi: 10.3791/889
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Allowed tags: i, b, u, sup, sub 
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| | 12/19/2008 8:54:32 AM
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Maria V. Miranda responded with a statement of type: Neutral
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I would like to know who sells the syringe-aplicator like you show in this video
Thank you
MV Miranda |
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| | 01/29/2009 10:42:17 AM
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Wendy Sparks responded with a statement of type: Neutral
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The one used in these protocols was a Burkard Microprocessor Controller Pax-100. I hope that will help you in your search.
W. O. Sparks |
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Mailer.URL: http://www.jove.com/index/details.stp?id=889 |
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