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Preparation and Fractionation of Xenopus laevis Egg Extracts
Marie K. Cross, Maureen Powers
Department of Cell Biology, Emory University

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0:00 Title0

0:21 Subscription Lock21

0:34 Introduction34

1:12 Lysing Eggs and Collecting Crude Extract72

3:36 Fractionating Crude Egg Extracts and Collecting Cytoplasmic Extract216

5:23 Collecting the Light Membrane Fraction of the Egg Extract323

7:02 Conclusion422
Abstract
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
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The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Cite this Article
K. Cross M, Powers M (2008). Preparation and Fractionation of Xenopus laevis Egg Extracts. JoVE. 18. http://www.jove.com/index/details.stp?id=891, doi: 10.3791/891
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08/27/2008

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doi: 10.3791/891 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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