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This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina.
Launch Video

     Published: 07/02/2008
Birgit Werner1
Paul B. Cook2, 1
Christopher L. Passaglia3, 1

1Program in Neuroscience, Boston University

2Department of Biology, Boston University

3Department of Biomedical Engineering, Boston University

 
Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.
Launch Video

     Published: 07/02/2008
Richard Ricardo
Katy Phelan

Molecular Pathology Laboratory Network, Inc

 
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
Launch Video

     Published: 06/27/2008
Kelly Morgan1
Michael Kharas1
Elaine Dzierzak2
D. Gary Gilliland1, 3

1Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School

2Department of Cell Biology and Genetics, Erasmus University Medical Center

3Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School

 
We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.
Launch Video

     Published: 06/24/2008
Christos Gekas
Katrin E. Rhodes
Hanna K. A. Mikkola

Jonsson Comprehensive Cancer Center, University of California, Los Angeles

 
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
Launch Video

1 Comment    Published: 06/23/2008
Richard Ricardo
Katy Phelan

Molecular Pathology Laboratory Network, Inc

 
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.
Launch Video

1 Comment    Published: 06/20/2008
Sean Gallagher1
Deb Chakavarti2

1UVP, LLC

2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

 
In this video, we will show you how the mitochondrial respiratory chain complexes of human embryonic stem cells can be analyzed using in gel activity assays.
Launch Video

     Published: 06/17/2008
Ivan Khvorostov
Jin Zhang
Michael Teitell

David Geffen School of Medicine, University of California, Los Angeles

 
This video describes the method of retrograde labeling of RGC by applying fluoro-gold (FG) on the surface of superior colliculus (SC). Technique involves drilling the skull, aspirating the cortex, and applying gelatin sponge over entire dorsal surface of SC.
Launch Video

     Published: 06/17/2008
Kin Chiu
Wui-Man Lau
Sze-chun Yeung
Raymond Chuen-Chung Chang
Kwok-Fai So

University of Hong Kong

 
Measuring the yaw torque of tethered Drosophila with the torque meter allows the neuroscientist exquisite control of the stimulus situation of the experimental animal. Together with the unique genetic tools available in the fruit fly, this paradigm is used for a wide variety of neurobiological research.
Launch Video

1 Comment    Published: 06/16/2008
Bjorn Brembs

Department of Neurobiology, Free University of Berlin

 
Current HIV-1 strategies act to suppress the viral life cycle but do not effectively eradicate infection. Here, we demonstrate that an engineered recombinase can efficiently excise integrated HIV-1 proviral DNA from the genome of infected cells.
Launch Video

2 Comments    Published: 06/16/2008
Joachim Hauber

Department of Cell Biology and Virology, Heinrich-Pette-Institute for Experimental Virology and Immunology, University of Hamburg

 

 

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Total: 206 articles.

 

Search results for 7/4/2008 9:21:16 AM

 
 

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