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Journal of Visualized Experiments

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Issue: 30

Page 1 2 3

Video Articles

Authors

Keywords

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

A new means to measure neurotransmission optically using fluorescent dopamine analogs.

Launch Video

1 Comment Published: 08/31/2009

Hui Zhang^{1, 2}
Niko G. Gubernator^{3, 4}
Minerva Yue¹
Roland G. W. Staal¹
Eugene V. Mosharov¹
Daniela Pereira¹
Vojtech Balsanek³
Paul A. Vadola³
Bipasha Mukherjee⁵
Robert H. Edwards⁵
David Sulzer^{1, 2, 6}
Dalibor Sames³

¹Departments of Neurology, Columbia University

²Departments of Psychiatry and Pharmacology, Columbia University

³Department of Chemistry, Columbia University

⁴eMolecules, Inc.

⁵Departments of Neurology and Physiology, University of California School of Medicine, San Francisco

⁶Division of Molecular Therapeutics, New York Psychiatric Institute

Neuroscience, Issue 30, striatal slice, dopamine, synapse, synaptic vesicles, amphetamine, optical imaging, fluorescence, release

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.

Launch Video

3 Comments Published: 08/28/2009

Kenneth K. Kwong^{1, 2}
Lenuta Kloetzer^{1, 2, 3, 4}
Kelvin K. Wong^{5, 6}
Jia-Qian Ren^{1, 2}
Braden Kuo^{1, 2, 3, 4}
Yan Jiang⁷
Y. Iris Chen^{1, 2}
Suk-Tak Chan^{8, 1, 2}
Geoffrey S. Young⁹
Stephen T.C. Wong^{5, 6}

¹Department of Radiology, Massachusetts General Hospital, Harvard Medical School

²Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School

³Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School

⁴Department of Medicine, Massachusetts General Hospital, Harvard Medical School

⁵Center for biotechnology and Informatics, The Methodist Hospital Research Institute

⁶Department of Radiology, The Methodist Hospital, Weill Cornell Medical College

⁷Bejing University of Chinese Medicine

⁸Department of Health Technology and Informatics, The Hong Kong Polytechnic University

⁹Department of Radiology, Brigham and Women’s Hospital, Harvard Medical School

Medicine, Issue 30, Gua Sha, blood extravasation, bruises, heme oxygenase-1, gene expression, systems biology, small animal molecular imaging, optical and bioluminescence imaging, HO-1-luciferase transgenic mice, Chinese folk therapy

In vivo Imaging of Deep Cortical Layers using a Microprism

Right-angle microprisms inserted into the mouse neocortex allows for deep imaging of multiple cortical layers with a viewpoint typically found in slice. One-millimeter microprisms offer a wide field-of-view (~900 μm) and spatial resolutions sufficient to resolve dendritic spines. We demonstrate layer V neuronal imaging and neocortical vascular imaging using microprisms.

Launch Video

Published: 08/27/2009

Thomas H. Chia
Michael J. Levene

Department of Biomedical Engineering, Yale University

Neuroscience, Issue 30, Cortex, Layer V, Multiphoton Microscopy, Brain, Mouse, Fluorescence, Microprism, Imaging, Neurovasculature, In vivo

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

We present a novel and powerful integration of nanophotonics (QD-FRET) and microfluidics to investigate the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

Launch Video

Published: 08/26/2009

Yi-Ping Ho^{1, 2}
Hunter H. Chen^{2, 3}
Kam W. Leong²
Tza-Huei Wang^{3, 1}

¹Mechanical Engineering, Johns Hopkins University

²Biomedical Engineering, Duke University

³Biomedical Engineering, Johns Hopkins University

Biomedical Engineering, Issue 30, microfluidics, gene delivery, quantum dots, fluorescence resonance energy transfer, self-assembly, nanocomplexes

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

We present a procedure for forming a poly(ethylene glycol) self-assembled monolayer (PEG-SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single step and prevents biofouling on silicon and gold surfaces. Electrophoresis is then used for patterning biomolecules down to the nanoscale.

Launch Video

Published: 08/25/2009

John Noel¹
Winfried Teizer¹
Wonmuk Hwang²

¹Department of Physics, Texas A & M University

²Department of Biomedical Engineering, Texas A & M University

Biomedical Engineering, Issue 30, protein patterning, self-assembly, tubulin, kinesin, biofouling, bioNEMS, biosensor

Purification of Mitochondria from Yeast Cells

We describe a rapid and effective method for purification of mitochondria from the yeast Saccharomyces cerevisiae. This method enables the high-yield isolation of pure mitochondria that are essentially free of contamination by other organelles and retain their structural and functional integrity after their purification.

Launch Video

5 Comments Published: 08/24/2009

Christopher Gregg
Pavlo Kyryakov
Vladimir I. Titorenko

Department of Biology, Concordia University

Cellular Biology, Issue 30, subcellular fractionation, organelles, organelle purification, mitochondria

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

We describe a new quantitative lipidomics method for identifying numerous lipid species in yeast using survey-scan electrospray ionization mass spectrometry (ESI/MS). This method exceeds currently available methods for lipid identification and quantification in the ability to resolve various molecular forms of lipids, sensitivity, and speed.

Launch Video

Published: 08/21/2009

Simon D. Bourque
Vladimir I. Titorenko

Department of Biology, Concordia University

Cellular Biology, Issue 30, mass spectrometry, lipidomics, lipid identification, lipid quantification

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

Launch Video

Published: 08/20/2009

Claudio Vinegoni¹
Daniel Razansky²
Chrysoula Pitsouli³
Norbert Perrimon³
Vasilis Ntziachristos²
Ralph Weissleder¹

¹Center for Systems Biology, Massachusetts General Hospital

²Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich

³Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

Developmental Biology, Issue 30, fluorescence tomography, mesoscopic imaging, Drosophila, optical imaging, diffusion tomography, scattering

Detection of Protein Ubiquitination

Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.

Launch Video

Published: 08/19/2009

Yeun Su Choo
Zhuohua Zhang

Signal Transduction Program, Burnham Institute for Medical Research

Cell Biology, Biochemistry, Issue 30, ubiquitination, cultured cell, in vitro system, immunoprecipitation, immunoblotting, ubiquitin, posttranslational modification

In vivo Ca²⁺- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee

Bees can be conditioned in an appetitive olfactory learning paradigm (PER-conditioning). Using odors as stimuli, we established a method in which behavior is recorded while simultaneously Calcium Imaging is used to measure odor evoked activity in mushroom body neurons in vivo.

Launch Video

3 Comments Published: 08/18/2009

Melanie Haehnel
Anja Froese
Randolf Menzel

Institut für Biologie - Neurobiologie, Freie Universität Berlin

Neuroscience, Issue 30, Calcium Imaging, Insects, Mushroom Body, PER Conditioning, Olfaction, Fura-2

Page:1 2 3

Total: 21 articles.

Search results for 7/31/2010 4:17:20 PM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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