Journal of Visualized Experiments

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Issue: 32

Page  1 2 3 

 

Video Articles

Authors

Keywords
Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.
Launch Video

    Published: 10/30/2009
Katharina Schallmoser
Dirk Strunk

Stem Cell Research Unit, Medical University of Graz, Austria

 
Denaturing urea polyacrylamide gel electrophoresis is used to separate single-stranded DNA or RNA up to a limit of 500 nucleotides. Urea in combination with heat denatures samples and unstructured single strands migrate within the gel matrix according to their molecular weight.
Launch Video

2 Comments    Published: 10/29/2009
Heike Summer1
René Grämer2
Peter Dröge1

1School of Biological Sciences, Nanyang Technological University, Singapore - NTU

2Singapore-MIT Alliance for Reserach and Technology (SMART)

 
Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.
Launch Video

2 Comments    Published: 10/28/2009
Nicole A. Hofmann
Andreas Reinisch
Dirk Strunk

Stem Cell Research Unit, Medical University of Graz, Austria

 
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
Launch Video

2 Comments    Published: 10/27/2009
Carolyn G. Conant
Michael A. Schwartz
Tanner Nevill
Cristian Ionescu-Zanetti

Fluxion Biosciences, Inc.

 
We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.
Launch Video

    Published: 10/26/2009
Sudhir Khetan
Jason Burdick

Department of Bioengineering, University of Pennsylvania

 
A protocol to detect trichothecenes (mycotoxins of concern for human health) using a newly developed screening method based on a competitive immunochemical method and a final electrochemical detection is demonstrated.
Launch Video

    Published: 10/23/2009
Daniela Romanazzo
Francesco Ricci
Silvia Vesco
Silvia Piermarini
Giulia Volpe
Danila Moscone
Giuseppe Palleschi

Department of Sciences and Chemical Technologies, University of Rome, Tor Vergata

 
The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.
Launch Video

    Published: 10/22/2009
Harold Moreno-Ortiz
Clara Esteban-Perez
Wael Badran
Marijo Kent-First

Reproductive Genetics and Stem Cell Laboratory, Department of Biological Sciences, Mississippi State University

 
The process of electrospinning polymers for tissue engineering and cell culture is addressed in this article. Specifically, the electrospinning of photoreactive macromers with additional processing capabilities of photopatterning and multi-polymer electrospinning is described.
Launch Video

    Published: 10/21/2009
Jamie L. Ifkovits
Harini G. Sundararaghavan
Jason A. Burdick

Department of Bioengineering, University of Pennsylvania

 
This work describes basic procedures of noninvasive small animal MRI and MRS in vivo.
Launch Video

    Published: 10/20/2009
Donghoon Lee1
David Marcinek1, 2

1Department of Radiology, University of Washington

2Department of Bioengineering, University of Washington

 
We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.
Launch Video

    Published: 10/19/2009
Marino DiFranco
Marbella Quinonez
Joana Capote
Julio Vergara

Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles

 

 

Page:1 2 3 

 

Total: 22 articles.

 

Search results for 7/31/2010 4:03:57 PM

 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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