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Video Articles

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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.

Launch Video

Published: 07/31/2010

Izhak Michaelevski
Noam Kirshenbaum
Michal Sharon

Department of Biological Chemistry, Weizmann Institute of Science

cellular biology, Issue 41, mass spectrometry, ion-mobility, protein complexes, non-covalent interactions, structural biology

Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies

This article describes the experimental procedures used to prepare rat tail tendons for biomechanical and mechanobiological studies. Several features of the main steps in preparation are demonstrated, beginning with extraction, cross-sectional area measurement, rinsing and loading into the bioreactor chamber.

Launch Video

Published: 07/30/2010

Amélie Bruneau
Nadia Champagne
Paule Cousineau-Pelletier
Gabriel Parent
Eve Langelier

Groupe PERSEUS, Faculté de Génie Département de génie mécanique, Université de Sherbrooke

bioengineering, Issue 41, Rat tail tendon, extraction, cross-section, optic micrometer, anchors, bioreactor, biomechanics, mechanobiology

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

This protocol describes how chromatin immunoprecipitation (ChIP) is used to study the dynamic alterations to the chromatin template that regulate transcription induced by a signal transduction pathway.

Launch Video

Published: 07/29/2010

Lauren J. Buro
Shaili Shah
Melissa A. Henriksen

Department of Biology, University of Virginia

Cellular Biology, Issue 41, chromatin, histone modification, transcription, antibody, cell culture, epigenetics, transcription factor, nucleosome

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Reduced/absent expression of Pms2 and/or ERCC1 in entire crypts is a frequent event within 10 cm on each side of colonic adenocarcinomas, likely the basis of a field defect with high mutability and progression to cancer. Deficiency in Ku86 or CcOI is much less frequent in these field defects.

Launch Video

Published: 07/28/2010

Huy Nguyen¹
Cristy Loustaunau¹
Alexander Facista¹
Lois Ramsey¹
Nadia Hassounah¹
Hilary Taylor¹
Robert Krouse^{2, 3}
Claire M. Payne^{1, 4}
V. Liana Tsikitis³
Steve Goldschmid⁵
Bhaskar Banerjee⁵
Rafael F. Perini⁵
Carol Bernstein¹

¹Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson

²Southern Arizona Veterans Affairs Health Care System, Tucson, AZ

³Department of Surgery, College of Medicine, University of Arizona, Tucson

⁴Biomedical Diagnostics and Research, Tucson, AZ

⁵Department of Medicine, College of Medicine, University of Arizona, Tucson

Cellular Biology, Issue 41, DNA Repair, Apoptosis, Field Defect, Colon Cancer, Pms2, ERCC1, Cytochrome c Oxidase Subunit I, Ku86, Immunohistochemistry, Cancer Resection

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.

Launch Video

Published: 07/27/2010

Colin J. Thalhofer¹
Joel W. Graff²
Laurie Love-Homan³
Suzanne M. Hickerson⁴
Noah Craft⁵
Stephen M. Beverley⁴
Mary E. Wilson^{6, 7}

¹Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center

²Department of Biochemistry, University of Iowa, and the VA Medical Center

³Department of Internal Medicine, University of Iowa

⁴Department of Molecular Microbiology, Washington University School of Medicine

⁵Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center

⁶Interdisciplinary Immunology Program, Iowa City VA Medical Center

⁷Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa

Microbiology, Issue 41, IVIS, Leishmania, in vivo imaging, parasite, transgenic, bioluminescence, luciferase, cutaneous leishmaniasis, visceral leishmaniasis

Primer-Free Aptamer Selection Using A Random DNA Library

SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.

Launch Video

Published: 07/26/2010

Weihua Pan¹
Ping Xin¹
Susan Patrick¹
Stacey Dean²
Christine Keating²
Gary Clawson^{3, 4}

¹Department of Pathology, Hershey Medical Center, Pennsylvania State University

²Department of Chemistry, Pennsylvania State University

³Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University

⁴Materials Research Institute, Pennsylvania State University

Cellular Biology, Issue 41, aptamer, selection, S100B, sandwich

Labeling and Imaging Cells in the Zebrafish Hindbrain

Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.

Launch Video

Published: 07/25/2010

Pradeepa Jayachandran¹
Elim Hong²
Rachel Brewster²

¹Center for Neuroscience, Children’s National Medical Center

²Department of Biological Sciences, University of Maryland Baltimore County

JoVE Neuroscience, Issue 41, development, zebrafish, embryo, brain, neural tube, microinjection, sectioning, time-lapse microscopy, confocal microscopy

Basics of Multivariate Analysis in Neuroimaging Data

The current article describes the basics of multivariate analysis and contrasts it to the more commonly used voxel-wise univariate analysis. Both types of analysis are applied to a clinical-neuroscience data set. Supplementary split-half simulations show better replication of the multivariate results in independent data sets.

Launch Video

Published: 07/24/2010

Christian Georg Habeck

Department of Neurology, Columbia University

JoVE Neuroscience, Issue 41, fMRI, PET, multivariate analysis, cognitive neuroscience, clinical neuroscience

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.

Launch Video

Published: 07/23/2010

Anyaporn Sawasdichai¹
Hsin-Tien Chen¹
Nazefah Abdul Hamid¹
Padma-Sheela Jayaraman²
Kevin Gaston¹

¹Department of Biochemistry, School of Medical Sciences, University of Bristol

²Division of Immunity and Infection, School of Medicine, University of Birmingham

cellular biology, Issue 41, protein localisation, subcellular fractionation, in situ, chromatin, nuclear matrix

Assessment of Ultrasonic Vocalizations During Drug Self-administration in Rats

Drug self-administration and ultrasonic vocalizations (USV) are used as behavioral assessments in animal research, but rarely in combination. The purpose of this article is to describe the advantages of recording USVs during drug self-administration procedures to assess affective responses to drug experience.

Launch Video

Published: 07/22/2010

Esther Y. Maier^{1, 2}
Sean T. Ma³
Allison Ahrens^{2, 4}
Timothy J. Schallert^{2, 4, 5}
Christine L. Duvauchelle^{1, 2, 4}

¹College of Pharmacy, Division of Pharmacology and Toxicology, University of Texas at Austin

²The Waggoner Center of Addiction and Alcohol Research, University of Texas at Austin

³Department of Psychology, University of Michigan

⁴Institute for Neuroscience, University of Texas at Austin

⁵Department of Psychology, University of Texas at Austin

JoVE Neuroscience, Issue 41, ultrasound, behavior, self-administration, emotionality, anticipation, reward

Page:1 2 3 4 5 6 7 8 9 10

Total: 686 articles.

Search results for 7/31/2010 4:04:50 PM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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