Journal of Visualized Experiments

Sign In
New Account Forgot Password
Search
Home Browse For Authors Subscribe
All By Issue By Category By Keyword Advanced Search
Keyword Search: Cellular Biology

Page  1 2 3 4 5 6 7 8 9 10 

 

Video Articles

Authors

Keywords
Ion mobility-mass spectrometry is an emerging gas-phase technology that separates ions, based on their collision cross-section and mass. The method provides three-dimensional information on the overall topology and shape of protein complexes. Here, we outline a basic procedure for instrument setting and optimization, calibration of drift times, and data interpretation.
Launch Video

    Published: 07/31/2010
Izhak Michaelevski
Noam Kirshenbaum
Michal Sharon

Department of Biological Chemistry, Weizmann Institute of Science

 
This protocol describes how chromatin immunoprecipitation (ChIP) is used to study the dynamic alterations to the chromatin template that regulate transcription induced by a signal transduction pathway.
Launch Video

    Published: 07/29/2010
Lauren J. Buro
Shaili Shah
Melissa A. Henriksen

Department of Biology, University of Virginia

 
Reduced/absent expression of Pms2 and/or ERCC1 in entire crypts is a frequent event within 10 cm on each side of colonic adenocarcinomas, likely the basis of a field defect with high mutability and progression to cancer. Deficiency in Ku86 or CcOI is much less frequent in these field defects.
Launch Video

    Published: 07/28/2010
Huy Nguyen1
Cristy Loustaunau1
Alexander Facista1
Lois Ramsey1
Nadia Hassounah1
Hilary Taylor1
Robert Krouse2, 3
Claire M. Payne1, 4
V. Liana Tsikitis3
Steve Goldschmid5
Bhaskar Banerjee5
Rafael F. Perini5
Carol Bernstein1

1Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson

2Southern Arizona Veterans Affairs Health Care System, Tucson, AZ

3Department of Surgery, College of Medicine, University of Arizona, Tucson

4Biomedical Diagnostics and Research, Tucson, AZ

5Department of Medicine, College of Medicine, University of Arizona, Tucson

 
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
Launch Video

    Published: 07/26/2010
Weihua Pan1
Ping Xin1
Susan Patrick1
Stacey Dean2
Christine Keating2
Gary Clawson3, 4

1Department of Pathology, Hershey Medical Center, Pennsylvania State University

2Department of Chemistry, Pennsylvania State University

3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University

4Materials Research Institute, Pennsylvania State University

 
In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.
Launch Video

    Published: 07/23/2010
Anyaporn Sawasdichai1
Hsin-Tien Chen1
Nazefah Abdul Hamid1
Padma-Sheela Jayaraman2
Kevin Gaston1

1Department of Biochemistry, School of Medical Sciences, University of Bristol

2Division of Immunity and Infection, School of Medicine, University of Birmingham

 
We demonstrate our approach to finding potential enhancer elements from developmentally regulated genes and evaluating their function through mosaic zebrafish transgenesis.
Launch Video

    Published: 07/16/2010
Erika Kague
Christopher Weber
Shannon Fisher

Department of Cell and Developmental Biology, University of Pennsylvania

 
The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.
Launch Video

    Published: 07/15/2010
Kate Wagner
David Welch

GIBCO, Life Technologies

 
This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.
Launch Video

    Published: 07/15/2010
Kate Wagner
David Welch

GIBCO, Life Technologies

 
Launch Video

    Published: 07/15/2010

 
Launch Video

    Published: 07/15/2010

 

 

Page:1 2 3 4 5 6 7 8 9 10 

 

Total: 183 articles.

 

Search results for 7/31/2010 4:11:51 PM

 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

Quick links

 

* Welcome  

* Browse  

* Submit  

* About  

* Editorial Board  

* Press  

* Advertising

* Contact  

* Policies

* Article Index

* Sponsors

* Press Access

* Troubleshooting

* Minerva

 

 

* Twitter.com/JoveJournal

* FaceBook

Subscribe
  RSS

You don't have to create an account to receive email notifications. 

Please enter your email:

Submit

Copyright (C) JoVE 2006-2008 All Rights Reserved.  Powered by TARGET.  JoVE Site Version 1.893