Journal of Visualized Experiments

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Keyword Search: Developmental Biology

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Video Articles

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We demonstrate a protocol to generate chimeric zebrafish embryos for live imaging cellular behavior during embryogenesis.
Launch Video

    Published: 07/19/2010
Jian Zou1
Xiangyun Wei1, 2

1Department of Ophthalmology, University of Pittsburgh

2Department of Microbiology and Molecular Genetics, University of Pittsburgh

 
In insects, the oenocytes produce cuticular hydrocarbon compounds. These compounds protect against desiccation and facilitate chemical communication. Here we demonstrate a dissection technique used to isolate the oenocytes from adult Drosophila melanogaster, and illustrate how this preparation can be utilized to study genes involved in hydrocarbon synthesis.
Launch Video

    Published: 07/18/2010
Joshua J. Krupp
Joel D. Levine

Department of Biology, University of Toronto

 
Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system.
Launch Video

    Published: 06/30/2010
Gianni Carraro
Pierre-Marie del Moral
David Warburton

Saban Research Institute, Childrens Hospital Los Angeles

 
This article describes an approach to microdissect zebrafish retinas with and without retinal pigment epithelium attached, from one to three days postfertilization embryos.
Launch Video

    Published: 06/27/2010
Liyun Zhang
Yuk Fai Leung

Department of Biological Sciences, Purdue University

 
Xenopus embryonic epithelia are an ideal model system to study cell behaviors such as polarity development and shape change during epithelial morphogenesis. Traditional histology of fixed samples is increasingly being complemented by live-cell confocal imaging. Here we demonstrate methods to isolate frog tissues and visualize live epithelial cells and their cytoskeleton using live-cell confocal microscopy.
Launch Video

    Published: 05/23/2010
Sagar D. Joshi1
Lance A. Davidson1, 2

1Bioengineering, University of Pittsburgh

2Developmental Biology, University of Pittsburgh

 
Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.
Launch Video

    Published: 05/16/2010
Andrea S. Viczian
Michael E. Zuber

Department of Ophthalmology, SUNY Upstate Medical University

 
Laser microdissection was applied to analyse gene expression profiling in specific compartments of Drosophila wing disc subjected to localised RNAi in vivo. RNA extracted from equivalent areas of silenced and unsilenced compartments was analysed by quantitative RT-PCR to determine comparative gene expression profiling within the context of native tissue microecology.
Launch Video

    Published: 04/30/2010
Rosario Vicidomini
Giuseppe Tortoriello
Maria Furia
Gianluca Polese

Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli Federico II

 
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
Launch Video

4 Comments    Published: 03/04/2010
Tripti Gupta
Mary C. Mullins

Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine

 
Drosophila hemocytes disperse over the entirety of the developing embryo. This protocol demonstrates how to mount and image these migrations using embryos with fluorescently labelled hemocytes.
Launch Video

1 Comment    Published: 02/12/2010
Iwan R. Evans1
Jennifer Zanet2
Will Wood1
Brian M. Stramer2

1Department of Biology and Biochemistry, University of Bath

2Randall Division of Cell and Molecular Biophysics, King's College London

 
This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.
Launch Video

1 Comment    Published: 02/03/2010
Erica Andersen1, 2, 3
Namrata Asuri1, 2, 3
Matthew Clay2, 3, 4
Mary Halloran1, 2, 3, 4

1Genetics Training Program, University of Wisconsin-Madison

2Department of Anatomy, University of Wisconsin-Madison

3Department of Zoology, University of Wisconsin-Madison

4Cell and Molecular Biology Training Program, University of Wisconsin-Madison

 

 

Page:1 2 3 4 5 6 7 8 

 

Total: 71 articles.

 

Search results for 7/31/2010 4:17:35 PM

 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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