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Keyword Search: Immunology

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Video Articles

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Orthotopic Hind-Limb Transplantation in Rats

Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.

Launch Video

1 Comment Published: 07/12/2010

Robert Sucher^1,*
Rupert Oberhuber^1,*
Christian Margreiter¹
Guido Rumberg¹
Rishi Jindal²
WP Andrew Lee²
Raimund Margreiter¹
Johann Pratschke¹
Stefan Schneeberger¹
Gerald Brandacher¹

¹Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University

²Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center
^* These authors contributed equally

JoVE Immunology, Issue 41, rat, hind limb, composite tissue, reconstructive transplantation

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.

Launch Video

Published: 07/10/2010

Sreemanti Basu
Hope M. Campbell
Bonnie N. Dittel
Avijit Ray

Blood Research Institute, BloodCenter of Wisconsin

JoVE Immunology, Issue 41, cell sorting, monoclonal antibodies, compensation, antibody titration, FACS

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.

Launch Video

Published: 07/08/2010

Christina Farr¹
Stuart Berger^{1, 2}

¹Immunology, University of Toronto

²Arthritis and Immune Disorder Research Centre, University Health Network

JoVE Immunology, Issue 41, calpain, immunology, flow cytometry, acute myeloid leukemia

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.

Launch Video

Published: 06/01/2010

Craig T. Lefort
Minsoo Kim

Center for Vaccine Biology and Immunology, University of Rochester

JoVE Immunology, Issue 40, T lymphocyte, Migration, Integrin, LFA-1, ICAM-1, Chemokine

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.

Launch Video

1 Comment Published: 04/22/2010

Hong He¹
Amy N. Courtney¹
Eric Wieder²
K. Jagannadha Sastry¹

¹Department of Immunology, The University of Texas M.D. Anderson Cancer Center

²Department of Medicine, University of Miami

JoVE Immunology, Issue 38, Immune Response, Cytokine Production, Flow Cytometry, HIV, Rhesus Macaque, T Cells, Intracellular Cytokine Staining, FACS

Measuring the 50% Haemolytic Complement (CH₅₀) Activity of Serum

The classical pathway is activated by antibody and culminates in target cell lysis. The CH₅₀ assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH₅₀ of a serum sample, the calculations and interpreting of results.

Launch Video

Published: 03/29/2010

Maurizio Costabile

School of Pharmacy and Medical Sciences, University of South Australia

Jove Immunology, Issue 37, Classical pathway, Complement, Haemolysis, sheep red blood cells, haemoglobin

Murine Model of CD40-activation of B cells

In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.

Launch Video

2 Comments Published: 03/05/2010

Tanja M. Liebig
Anne Fiedler
Nela Klein-Gonzalez
Alexander Shimabukuro-Vornhagen
Michael von Bergwelt-Baildon

Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne

JoVE Immunology, Issue 37, murine CD40-activated B cell, B cell, antigen presentation, APC, immunotherapy, cancer vaccine

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.

Launch Video

1 Comment Published: 02/24/2010

Volker Brinkmann¹
Britta Laube¹
Ulrike Abu Abed^{1, 2}
Christian Goosmann^{1, 2}
Arturo Zychlinsky²

¹Core Facility Microscopy, Max Planck Institute for Infection Biology

²Cellular Microbiology, Max Planck Institute for Infection Biology

JoVE Immunology, Issue 36, Neutrophil, Granulocyte, Neutrophil Extracellular Trap, NET, isolation, immunolabeling, electron microscopy

Depletion of Specific Cell Populations by Complement Depletion

To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

Launch Video

Published: 02/05/2010

Bonnie N. Dittel

BloodCenter of Wisconsin, Blood Research Institute

JoVE Immunology, Issue 36, rabbit, complement, cell isolation, cell depletion

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.

Launch Video

Published: 01/29/2010

Maurizio Costabile

School of Pharmacy and Medical Sciences, University of South Australia

JoVE Immunology, Issue 35, Haemagglutination, Titre, Reactivity, Ag-Ab complex

Page:1 2 3 4 5 6

Total: 53 articles.

Search results for 7/31/2010 4:03:11 PM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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