Journal of Visualized Experiments

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This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.
Launch Video

    Published: 07/17/2010

Simon Rajendran1
Slawomir Salwa1
Xuefeng Gao2
Sabin Tabirca2
Deirdre O'Hanlon3
Gerald C. O'Sullivan1
Mark Tangney1

1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork

2Department of Computer Science, University College Cork

3South Infirmary Victoria University Hospital

 

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.
Launch Video

    Published: 07/09/2010

Surya C. Gnyawali1, 2
Sashwati Roy1, 2
Jason Driggs1, 2
Savita Khanna1, 2
Thomas Ryan3, 2
Chandan K. Sen2

1Department of Surgery, The Ohio State University

2Heart and Lung Research Institute, The Ohio State University

3Department of Cardiovascular Medicine, The Ohio State University

 

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.
Launch Video

    Published: 06/22/2010

Douglas Nam1
Chih-Wen Ni2
Amir Rezvan1
Jin Suo2
Klaudia Budzyn1
Alexander Llanos1
David G. Harrison1
Don P. Giddens2
Hanjoong Jo1, 2, 3

1Department of Medicine, Division of Cardiology, Emory University

2Coulter Department of Biomedical Engineering, Georgia Tech and Emory University

3Department of Bioinspired Science, Ewha Womans University

 

The structure and process for measuring student learning outcomes through customizable assessment rubrics is discussed and applied to a doctoral nursing education program.
Launch Video

    Published: 06/21/2010

Glenn H. Raup1
Jeff King2
Romana J. Hughes2
Natasha Faidley2

1Harris College of Nursing and Health Sciences

2Koehler Center for Teaching Excellence, Texas Christian University

 

The present article presents the details pertaining to the application of resistance training associated to vascular occlusion in IBM patients.
Launch Video

    Published: 06/05/2010

Bruno Gualano1, 2
Carlos Ugrinowitsch1
Manoel Neves Jr.2
Fernanda R. Lima2
Ana Lúcia S. Pinto2
Gilberto Laurentino1
Valmor A.A. Tricoli1
Antonio H. Lancha Jr.1
Hamilton Roschel1, 2

1School of Physical Education and Sport, University of São Paulo

2Division of Rheumatology, School of Medicine, University of São Paulo

 

Transthoracic echocardiography offers a noninvasive method for the evaluation of cardiac function in mice. A combination of ultrasound and Doppler imaging modalities can be used to obtain dimensional measurements of the heart and intracardiac blood flow, which together provide an assessment of cardiac systolic and diastolic performance.
Launch Video

    Published: 05/28/2010

Jonathan L. Respress1
Xander H.T. Wehrens1, 2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

 

Telemetric ECG has emerged as an essential tool in evaluating animal models for cardiac arrhythmias and sudden cardiac death. Here, we present a stepwise guide to telemetric ECG recordings for application in long-term ambulatory ECG monitoring in mice.
Launch Video

    Published: 05/27/2010

Mark D. McCauley1, 2
Xander H.T Wehrens1, 2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

 

Programmed electrical stimulation provides the ability to determine conduction properties of the heart, and the possibility to induce and terminate cardiac arrhythmias using various pacing protocols. Using a transvenous catheter, intracardiac electrogram recordings can be obtained in mice following programmed electrical stimulation protocols to identify arrhythmogenic substrates.
Launch Video

    Published: 05/26/2010

Na Li1
Xander H.T Wehrens1, 2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

 

This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.
Launch Video

1 Comment    Published: 05/15/2010

Shah M. R. Karim1
Chin T. Ong2
Tamsin J. Sleep3

1Department of Ophthalmology, Hairmyres Hospital, NHS Lanarkshire

2Royal Devon and Exeter NHS Foundation Trust, Department of Ophthalmology

3Department of Ophthalmology, South Devon Healthcare NHS Trust

 

Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model to study mechanisms underlying cardiac hypertrophy and heart failure development. Here, we describe procedures to constrict the aorta to create a reproducible degree of cardiac hypertrophy in mice.
Launch Video

5 Comments    Published: 04/21/2010

Angela C. deAlmeida1
Ralph J. van Oort1
Xander H.T. Wehrens1, 2

1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

2The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

 

 

Page:1 

 

Total: 51 articles.

 

Search results for 7/31/2010 4:19:35 PM

 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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