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Murine Bioluminescent Hepatic Tumour Model

This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

Launch Video

Published: 07/17/2010

Simon Rajendran¹
Slawomir Salwa¹
Xuefeng Gao²
Sabin Tabirca²
Deirdre O'Hanlon³
Gerald C. O'Sullivan¹
Mark Tangney¹

¹Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork

²Department of Computer Science, University College Cork

³South Infirmary Victoria University Hospital

JoVE Medicine, Issue 41, Cancer, Therapy, Liver, Orthotopic, Metastasis

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.

Launch Video

Published: 07/09/2010

Surya C. Gnyawali^{1, 2}
Sashwati Roy^{1, 2}
Jason Driggs^{1, 2}
Savita Khanna^{1, 2}
Thomas Ryan^{3, 2}
Chandan K. Sen²

¹Department of Surgery, The Ohio State University

²Heart and Lung Research Institute, The Ohio State University

³Department of Cardiovascular Medicine, The Ohio State University

JoVE Medicine, Issue 41, ischemia-reperfused murine heart, high frequency ultrasound, heart contractility (dP/dt), mitral regurgitation

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Launch Video

Published: 06/22/2010

Douglas Nam¹
Chih-Wen Ni²
Amir Rezvan¹
Jin Suo²
Klaudia Budzyn¹
Alexander Llanos¹
David G. Harrison¹
Don P. Giddens²
Hanjoong Jo^{1, 2, 3}

¹Department of Medicine, Division of Cardiology, Emory University

²Coulter Department of Biomedical Engineering, Georgia Tech and Emory University

³Department of Bioinspired Science, Ewha Womans University

JoVE Medicine, Issue 40, atherosclerosis, disturbed flow, shear stress, carotid, partial ligation, endothelial RNA

Using Learning Outcome Measures to assess Doctoral Nursing Education

The structure and process for measuring student learning outcomes through customizable assessment rubrics is discussed and applied to a doctoral nursing education program.

Launch Video

Published: 06/21/2010

Glenn H. Raup¹
Jeff King²
Romana J. Hughes²
Natasha Faidley²

¹Harris College of Nursing and Health Sciences

²Koehler Center for Teaching Excellence, Texas Christian University

JoVE Medicine, Issue 40, learning, outcomes, measurement, program, assessment, rubric

Vascular Occlusion Training for Inclusion Body Myositis: A Novel Therapeutic Approach

The present article presents the details pertaining to the application of resistance training associated to vascular occlusion in IBM patients.

Launch Video

Published: 06/05/2010

Bruno Gualano^{1, 2}
Carlos Ugrinowitsch¹
Manoel Neves Jr.²
Fernanda R. Lima²
Ana Lúcia S. Pinto²
Gilberto Laurentino¹
Valmor A.A. Tricoli¹
Antonio H. Lancha Jr.¹
Hamilton Roschel^{1, 2}

¹School of Physical Education and Sport, University of São Paulo

²Division of Rheumatology, School of Medicine, University of São Paulo

JoVE Medicine, Issue 40, exercise training, therapeutical, myositis, vascular occlusion

Transthoracic Echocardiography in Mice

Transthoracic echocardiography offers a noninvasive method for the evaluation of cardiac function in mice. A combination of ultrasound and Doppler imaging modalities can be used to obtain dimensional measurements of the heart and intracardiac blood flow, which together provide an assessment of cardiac systolic and diastolic performance.

Launch Video

Published: 05/28/2010

Jonathan L. Respress¹
Xander H.T. Wehrens^{1, 2}

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine

²The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

JoVE Medicine, Issue 39, Echocardiography, pulse wave Doppler, tissue Doppler imaging, ultrasound

Ambulatory ECG Recording in Mice

Telemetric ECG has emerged as an essential tool in evaluating animal models for cardiac arrhythmias and sudden cardiac death. Here, we present a stepwise guide to telemetric ECG recordings for application in long-term ambulatory ECG monitoring in mice.

Launch Video

Published: 05/27/2010

Mark D. McCauley^{1, 2}
Xander H.T Wehrens^{1, 2}

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine

²The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

Medicine, Issue 39, Electrocardiogram, electrophysiology, exercise-stress test, mouse, telemetry

Programmed Electrical Stimulation in Mice

Programmed electrical stimulation provides the ability to determine conduction properties of the heart, and the possibility to induce and terminate cardiac arrhythmias using various pacing protocols. Using a transvenous catheter, intracardiac electrogram recordings can be obtained in mice following programmed electrical stimulation protocols to identify arrhythmogenic substrates.

Launch Video

Published: 05/26/2010

Na Li¹
Xander H.T Wehrens^{1, 2}

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine

²The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

JoVE Medicine, Issue 39, Arrhythmias, electrophysiology, mouse, programmed electrical stimulation

A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome

This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.

Launch Video

1 Comment Published: 05/15/2010

Shah M. R. Karim¹
Chin T. Ong²
Tamsin J. Sleep³

¹Department of Ophthalmology, Hairmyres Hospital, NHS Lanarkshire

²Royal Devon and Exeter NHS Foundation Trust, Department of Ophthalmology

³Department of Ophthalmology, South Devon Healthcare NHS Trust

JoVE Medicine, Issue 39, Phacoemulsification surgery, cataract surgery, capsulorhexis, capsulotomy, technique, Continuous curvilinear capsulorhexis, cystotome

Transverse Aortic Constriction in Mice

Transverse aortic constriction (TAC) in the mouse is a commonly used experimental model to study mechanisms underlying cardiac hypertrophy and heart failure development. Here, we describe procedures to constrict the aorta to create a reproducible degree of cardiac hypertrophy in mice.

Launch Video

5 Comments Published: 04/21/2010

Angela C. deAlmeida¹
Ralph J. van Oort¹
Xander H.T. Wehrens^{1, 2}

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine

²The Margaret M. and Albert B. Alkek Department of Medicine, Baylor College of Medicine

Medicine, Issue 38, Aorta, heart failure, hypertrophy, mouse, pressure-overload

Page:1 2 3 4 5 6

Total: 51 articles.

Search results for 7/31/2010 4:19:35 PM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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