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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.

Launch Video

Published: 07/27/2010

Colin J. Thalhofer¹
Joel W. Graff²
Laurie Love-Homan³
Suzanne M. Hickerson⁴
Noah Craft⁵
Stephen M. Beverley⁴
Mary E. Wilson^{6, 7}

¹Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center

²Department of Biochemistry, University of Iowa, and the VA Medical Center

³Department of Internal Medicine, University of Iowa

⁴Department of Molecular Microbiology, Washington University School of Medicine

⁵Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center

⁶Interdisciplinary Immunology Program, Iowa City VA Medical Center

⁷Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa

Microbiology, Issue 41, IVIS, Leishmania, in vivo imaging, parasite, transgenic, bioluminescence, luciferase, cutaneous leishmaniasis, visceral leishmaniasis

Method for the Isolation of Francisella tularensis Outer Membranes

A protocol for separating inner and outer membranes from Francisella tularensis by spheroplasting, osmotic lysis, and sucrose density gradient ultracentrifugation.

Launch Video

Published: 06/29/2010

Jason F. Huntley
Gregory T. Robertson
Michael V. Norgard

Department of Microbiology, University of Texas Southwestern Medical Center

Microbiology, Issue 40, Francisella, tularemia, outer membrane protein, sucrose density gradient centrifugation, membrane isolation, osmotic lysis, spheroplast

Chronic Salmonella Infected Mouse Model

Establish a chronic bacterial infected mouse model with persistent Salmonella typhimurium colonization in intestine for 27 weeks.

Launch Video

Published: 05/31/2010

Shaoping Wu^*
Rong Lu^*
Yong-guo Zhang
Jun Sun

Department of Medicine, University of Rochester
^* These authors contributed equally

Microbiology, Issue 39, Salmonella, intestine, colitis, chronic infection, mouse model

Electron Cryotomography of Bacterial Cells

We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

Launch Video

Published: 05/06/2010

Songye Chen¹
Alasdair McDowall^{1, 2}
Megan J. Dobro¹
Ariane Briegel^{1, 2}
Mark Ladinsky^{1, 2}
Jian Shi²
Elitza I. Tocheva¹
Morgan Beeby^{1, 2}
Martin Pilhofer^{1, 2}
H. Jane Ding¹
Zhuo Li^{1, 2}
Lu Gan¹
Dylan M. Morris¹
Grant J. Jensen^{1, 2}

¹Division of Biology, California Institute of Technology - Caltech

²Howard Hughes Medical Institute, California Institute of Technology - Caltech

Cellular Biology, Issue 39, Electron cryotomography, microbiology, bacteria, electron microscopy

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media

Microfluidic devices can be used to visualize complex natural processes in real time and at the appropriate physical scales. We have developed a simple microfluidic device that mimics key features of natural porous media for studying growth and transport of bacteria in the subsurface.

Launch Video

Published: 05/03/2010

Dmitry A. Markov^{1, 2}
Philip C. Samson¹
David K. Schaffer¹
Adit Dhummakupt¹
John P. Wikswo^{1, 2, 3, 4}
Leslie M. Shor^{5, 6}

¹Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University

²Department of Biomedical Engineering, Vanderbilt University

³Department of Molecular Physiology and Biophysics, Vanderbilt University

⁴Department of Physics and Astronomy, Vanderbilt University

⁵Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut

⁶Center for Environmental Sciences and Engineering, University of Connecticut

Microbiology, Issue 39, Microfluidic device, bacterial transport, porous media, colloid, biofilm, filtration theory, artificial habitat, micromodel, PDMS, GFP

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

We present a protocol for bending filamentous bacterial cells attached to a cover-slip surface with an optical trap to measure the cellular bending stiffness.

Launch Video

Published: 04/26/2010

Siyuan Wang¹
Hugo Arellano-Santoyo²
Peter A. Combs²
Joshua W. Shaevitz²

¹Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University

²Department of Physics, Lewis-Sigler Institute for Integrative Genomics, Princeton University

Microbiology, Issue 38, optical trap, cell mechanics, E. coli, cell bending

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial strains.

Launch Video

Published: 04/20/2010

Jeongyun Kim¹
Manjunath Hegde¹
Arul Jayaraman^{1, 2}

¹McFerrin Department of Chemical Engineering, Texas A&M University

²Department of Biomedical Engineering, Texas A&M University

Microbiology, Issue 38, Host pathogen interactions, probiotics, inter-kingdom signaling

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

Launch Video

Published: 04/19/2010

Derek L. Englert¹
Michael D. Manson²
Arul Jayaraman^{1, 3}

¹McFerrin Department of Chemical Engineering, Texas A&M University

²Department of Biology, Texas A&M University

³Department of Biomedical Engineering, Texas A&M University

Microbiology, Issue 38, chemotaxis, microfluidics, gradients

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

In this video, we describe a procedure for the expression of bacterial type III effectors in yeast and the identification of effector-induced growth inhibition phenotypes. Such phenotypes can be subsequently exploited to elucidate effector functions and targets.

Launch Video

Published: 03/30/2010

Dor Salomon
Guido Sessa

Department of Plant Sciences, Tel-Aviv University

Microbiology, Issue 37, type III secretion system, type III effector proteins, Gram-negative bacteria, Saccharomyces cerevisiae, yeast expression system

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

Launch Video

Published: 03/24/2010

Debarati Mukherjee
Arpita Sen
R. Claudio Aguilar

Department of Biological Sciences and Purdue Center for Cancer Research, Purdue University

Microbiology, Issue 37, Morphological phenotype, protein concentration, live cell imaging, cell division

Page:1 2 3 4 5

Total: 44 articles.

Search results for 7/31/2010 4:04:45 PM

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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