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Measuring the 50% Haemolytic Complement (CH₅₀) Activity of Serum

The classical pathway is activated by antibody and culminates in target cell lysis. The CH₅₀ assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH₅₀ of a serum sample, the calculations and interpreting of results.

Launch Video

Published: 03/29/2010

Maurizio Costabile

School of Pharmacy and Medical Sciences, University of South Australia

Jove Immunology, Issue 37, Classical pathway, Complement, Haemolysis, sheep red blood cells, haemoglobin

CoolCell - Controlled Cell Freezing w/o Alcohol or Maintenance Cost - ADVERTISEMENT

Launch Video

4 Comments Published: 01/14/2010

Rolf Ehrhardt
Brian Schryver
Jeff Schryver

Biocision

CoolCell, Cell Freezing, Cryopreservation, Cell lines, Cell Freezing Container, Cell based assay, Cell based therapy, Blood Bank, Freezing, Stem Cells, Patient Samples, Blood, PBMC, Mammalian Cell lines, Biostorage

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.

Launch Video

Published: 12/31/2009

William N. White¹
Palaniappan Sethu²

¹Department of Mechanical Engineering, University of Louisville

²Department of Bioengineering, University of Louisville

Cellular Biology, Issue 34, Microfluidics, Blood lysis, Cell Enrichment, Circulating Nucleated Cells, leukocytes, flow cytometry, FACS

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensfree on-chip imaging and characterization of cells is illustrated. This on-chip cell imaging approach provides a compact and cost-effective tool for medical diagnostics and high-throughput cell biology applications, making it especially suitable for resource poor settings.

Launch Video

Published: 12/14/2009

Onur Mudanyali¹
Anthony Erlinger¹
Sungkyu Seo¹
Ting-Wei Su¹
Derek Tseng¹
Aydogan Ozcan^{1, 2}

¹Electrical Engineering Department, University of California, Los Angeles

²California NanoSystems Institute, University of California, Los Angeles

Cellular Biology, Issue 34, LUCAS, lensfree imaging, on-chip imaging, point-of-care diagnostics, global health, cell-biology, telemedicine, wireless health, microscopy, red blood cells

The 2009 Lindau Nobel Laureate Meeting: Peter Agre, Chemistry 2003

American chemist Peter Agre shared the 2003 Nobel Prize in Chemistry for his discovery of aquaporins, the channel proteins that allow water to cross the cell membrane.

Launch Video

Published: 12/09/2009

Peter Agre

Cellular Biology, Issue 34, aquaporins, water-channel, diffusion, renal tubules, red blood cells

Retro-orbital Injection in Adult Zebrafish

Here we show how to do retro-orbital injection in adult zebrafish.

Launch Video

Published: 12/07/2009

Emily K. Pugach^{1, 2}
Pulin Li^{1, 2}
Richard White^{1, 2, 3}
Leonard Zon^{1, 2}

¹Department of Hematology and Oncology, Children’s Hospital Boston

²Harvard Stem Cell Institute, Harvard Medical School

³Department of Medical Oncology, Dana Farber Cancer Institute

Cellular Biology, Issue 34, fluorescent dye, kidney marrow cells, vasculature, red blood cells, Zebrafish, injection, retro-orbital injection, transplantation, HSC

Aortic Ring Assay

Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.

Launch Video

2 Comments Published: 11/24/2009

Keren Bellacen
Eli C. Lewis

Department Clinical Biochemistry, Ben-Gurion University of the Negev

Medicine, Issue 33, aortic rings, angiogenesis, blood vessels, aorta, mouse, vessel outgrowth

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.

Launch Video

2 Comments Published: 10/27/2009

Carolyn G. Conant
Michael A. Schwartz
Tanner Nevill
Cristian Ionescu-Zanetti

Fluxion Biosciences, Inc.

Medicine, Issue 32, thrombus formation, anti-thrombotic, microfluidic, whole blood assay, IC50, drug screening, platelet, adhesion

Microsurgical Clip Obliteration of Middle Cerebral Aneurysm Using Intraoperative Flow Assessment

Description of the surgical obliteration of a cerebral aneurysm utilizing an ultrasonic flow probe to assess arterial flow prior to and after aneurysm clip placement.

Launch Video

Published: 09/25/2009

Bob S. Carter
Christopher Farrell
Christopher Owen

Department of Neurosurgery, Havard Medical School, Massachusetts General Hospital

Medicine, Issue 31, Aneurysm, intraoperative, brain, surgery, surgical clipping, blood flow, aneurysmal segment, ultrasonic flow probe

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.

Launch Video

3 Comments Published: 08/28/2009

Kenneth K. Kwong^{1, 2}
Lenuta Kloetzer^{1, 2, 3, 4}
Kelvin K. Wong^{5, 6}
Jia-Qian Ren^{1, 2}
Braden Kuo^{1, 2, 3, 4}
Yan Jiang⁷
Y. Iris Chen^{1, 2}
Suk-Tak Chan^{8, 1, 2}
Geoffrey S. Young⁹
Stephen T.C. Wong^{5, 6}

¹Department of Radiology, Massachusetts General Hospital, Harvard Medical School

²Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School

³Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School

⁴Department of Medicine, Massachusetts General Hospital, Harvard Medical School

⁵Center for biotechnology and Informatics, The Methodist Hospital Research Institute

⁶Department of Radiology, The Methodist Hospital, Weill Cornell Medical College

⁷Bejing University of Chinese Medicine

⁸Department of Health Technology and Informatics, The Hong Kong Polytechnic University

⁹Department of Radiology, Brigham and Women’s Hospital, Harvard Medical School

Medicine, Issue 30, Gua Sha, blood extravasation, bruises, heme oxygenase-1, gene expression, systems biology, small animal molecular imaging, optical and bioluminescence imaging, HO-1-luciferase transgenic mice, Chinese folk therapy

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Total: 20 articles.

Search results for 9/10/2010 10:56:45 AM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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