Journal of Visualized Experiments

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Keyword Search: Imaging

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Video Articles

Authors

Keywords
Laser ablation electrospray ionization (LAESI) is an atmospheric-pressure ion source for mass spectrometry. In the imaging mode, a mid-infrared laser probes the distributions of molecules across a tissue section or a biofilm. This technique presents a new approach for diverse bioanalytical studies carried out under native experimental conditions.
Launch Video

    Published: 09/03/2010
Peter Nemes
Akos Vertes

Department of Chemistry, George Washington University

 
Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.
Launch Video

    Published: 08/28/2010
Masanori Takahashi1
Noriko Osumi1, 2

1Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine

2The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

 
This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.
Launch Video

    Published: 08/26/2010
Revital Halevy
Lazar Shtirberg
Michael Shklyar
Aharon Blank

Schulich Faculty of Chemistry, The Technion, Israel Institute of Technology

 
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
Launch Video

    Published: 08/17/2010
Alexis B. Cordero1
Youngjoo Kwon1
Xiang Hua2
Andrew K. Godwin1

1Department of Medical Oncology, Women's Cancer Program

2Transgenic Mouse Facility, Fox Chase Cancer Center

 
Thorough preclinical testing of drugs that act in the central nervous system often involves assessing and comparing drug biodistribution in association with specific routes of administration. Here, three commonly used methods of systemic delivery (intravenous, intraperitoneal, and oral) as well as a method for local delivery (convection-enhanced delivery) are demonstrated in mice.
Launch Video

    Published: 08/16/2010
Laura Serwer
Rintaro Hashizume
Tomoko Ozawa
C. David James

Department of Neurological Surgery, University of California, San Francisco

 
This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.
Launch Video

    Published: 08/08/2010
Andrew Pistner1
Stephen Belmonte2
Tonya Coulthard3
Burns Blaxall4, 2

1Department of Pharmacology and Physiology, University of Rochester

2Aab Cardiovascular Research Institute, University of Rochester

3Visualsonics

4Department of Medicine, University of Rochester

 
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
Launch Video

    Published: 07/27/2010
Colin J. Thalhofer1
Joel W. Graff2
Laurie Love-Homan3
Suzanne M. Hickerson4
Noah Craft5
Stephen M. Beverley4
Mary E. Wilson6, 7

1Interdisciplinary Immunology Program, University of Iowa, and the VA Medical Center

2Department of Biochemistry, University of Iowa, and the VA Medical Center

3Department of Internal Medicine, University of Iowa

4Department of Molecular Microbiology, Washington University School of Medicine

5Division of Dermatology, Harbor-UCLA Medical Center, Hanley-Hardison Research Center

6Interdisciplinary Immunology Program, Iowa City VA Medical Center

7Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa

 
We demonstrate a protocol to generate chimeric zebrafish embryos for live imaging cellular behavior during embryogenesis.
Launch Video

    Published: 07/19/2010
Jian Zou1
Xiangyun Wei1, 2

1Department of Ophthalmology, University of Pittsburgh

2Department of Microbiology and Molecular Genetics, University of Pittsburgh

 
Luciferase-modified human brain tumor xenografts can be established intracranially in athymic mice, with subsequent monitoring of tumor growth and response to therapy using bioluminescence imaging. In combination with survival analysis, bioluminescence monitoring is an essential research tool for pre-clinical testing of therapies being considered for treating brain tumors.
Launch Video

    Published: 07/13/2010
Tomoko Ozawa
C. David James

Department of Neurological Surgery, University of California, San Francisco

 
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
Launch Video

    Published: 06/26/2010
Gert-Jan Kremers
David Piston

Department of Molecular Physiology and Biophysics, Vanderbilt University

 

 

Page:1 2 3 4 5 6 

 

Total: 59 articles.

 

Search results for 9/10/2010 9:47:59 AM

 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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