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Murine Model of CD40-activation of B cells

In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.

Launch Video

2 Comments Published: 03/05/2010

Tanja M. Liebig
Anne Fiedler
Nela Klein-Gonzalez
Alexander Shimabukuro-Vornhagen
Michael von Bergwelt-Baildon

Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne

JoVE Immunology, Issue 37, murine CD40-activated B cell, B cell, antigen presentation, APC, immunotherapy, cancer vaccine

Generation of Human CD40-activated B cells

In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.

Launch Video

6 Comments Published: 10/16/2009

Tanja M. Liebig
Anne Fiedler
Shahram Zoghi
Alexander Shimabukuro-Vornhagen
Michael S. von Bergwelt-Baildon

Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine

Immunology, Issue 32, CD40-activated B cell, B cell, antigen presentation, APC, immunotherapy, cancer vaccine

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Total: 2 articles.

Search results for 9/10/2010 10:41:53 AM

About Jove

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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