Journal of Visualized Experiments

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3 Comments    Published: 07/12/2009

 
We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.
Launch Video

    Published: 02/18/2009
Rachel S. Poretsky
Scott Gifford
Johanna Rinta-Kanto
Maria Vila-Costa
Mary Ann Moran

Department of Marine Sciences, University of Georgia

 

 

Page:1 

 

Total: 2 articles.

 

Search results for 9/10/2010 11:25:50 AM

 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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