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Direct Tracheal Instillation of Solutes into Mouse Lung

Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice.

Launch Video

Published: 08/29/2010

My N. Helms^{1, 2}
Edilson Torres-Gonzalez^{3, 2}
Preston Goodson¹
Mauricio Rojas^{3, 2}

¹Department of Physiology, Emory University

²Center for Respiratory Health, Emory University

³Department of Medicine, Emory University

Medicine, Issue 42, trachea, instillation, distal lung, alveolar space, survival surgery

A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome

This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.

Launch Video

1 Comment Published: 05/15/2010

Shah M. R. Karim¹
Chin T. Ong²
Tamsin J. Sleep³

¹Department of Ophthalmology, Hairmyres Hospital, NHS Lanarkshire

²Royal Devon and Exeter NHS Foundation Trust, Department of Ophthalmology

³Department of Ophthalmology, South Devon Healthcare NHS Trust

JoVE Medicine, Issue 39, Phacoemulsification surgery, cataract surgery, capsulorhexis, capsulotomy, technique, Continuous curvilinear capsulorhexis, cystotome

Methods for the Study of the Zebrafish Maxillary Barbel

The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.

Launch Video

Published: 11/23/2009

Elizabeth E. LeClair¹
Jacek Topczewski²

¹Department of Biological Sciences, DePaul University

²Children’s Memorial Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine

Developmental Biology, Issue 33, zebrafish, regeneration, barbel, surgery, vasculature, circulation, imaging, agar, embedding, microscopy

Microsurgical Clip Obliteration of Middle Cerebral Aneurysm Using Intraoperative Flow Assessment

Description of the surgical obliteration of a cerebral aneurysm utilizing an ultrasonic flow probe to assess arterial flow prior to and after aneurysm clip placement.

Launch Video

Published: 09/25/2009

Bob S. Carter
Christopher Farrell
Christopher Owen

Department of Neurosurgery, Havard Medical School, Massachusetts General Hospital

Medicine, Issue 31, Aneurysm, intraoperative, brain, surgery, surgical clipping, blood flow, aneurysmal segment, ultrasonic flow probe

Implantation of Engineered Tissue in the Rat Heart

Here, we describe a cardiac surgical procedure to implant engineered tissue in the atrioventricular (AV)-groove of an adult Lewis rat.

Launch Video

4 Comments Published: 06/24/2009

Bjoern Sill
Ivan V. Alpatov
Christina A. Pacak
Douglas B. Cowan

Department of Anesthesiology, Perioperative and Pain Medicine, Children’s Hospital Boston and Harvard Medical School

Cellular Biology, Issue 28, thoracotomy, rodent surgery, anesthesia, atrioventricular, cardiac, tissue engineering, intubation

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Total: 5 articles.

Search results for 9/6/2010 7:42:00 PM

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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