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A Protocol for the Production of KLRG1 Tetramer

This protocol describes the production of KLRG1 tetramer, which is a powerful tool for the analysis of KLRG1 ligands.

Launch Video

Published: 01/12/2010

Stephanie C. Terrizzi
Cindy Banh
Laurent Brossay

Department of Molecular Microbiology and Immunology, Brown University

Microbiology, Issue 35, Immunology, Basic Protocols, Tetramer, Inclusion Bodies, Refolding, Monomer, Flow Cytometry, KLRG1, Cadherins

The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (German)

Launch Video

Published: 12/11/2009

Translation, Basic Protocols, Issue 33, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (Spanish)

Launch Video

1 Comment Published: 12/11/2009

Translation, Basic Protocols, Issue 33, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

The NanoDrop 2000c Spectrophotometer Microvolume Protein Concentration Determination (French)

Launch Video

Published: 12/11/2009

Translation, Basic Protocols, Issue 33, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.

Launch Video

2 Comments Published: 12/03/2009

Chuck Witkowski
Jay Harkins

Research and Development, Protein Discovery, Inc.

Basic Protocols, Cellular Biology, Issue 34, GELFREE, SDS PAGE, gel electrophoresis, protein fractionation, separation, electrophoresis, proteomics, mass spectrometry

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.

Launch Video

Published: 11/05/2009

Andrea Balbo
Huaying Zhao
Patrick H. Brown
Peter Schuck

National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science

Basic Protocols, Issue 33, analytical ultracentrifugation, sedimentation velocity, sedimentation equilibrium, protein characterization, sedimentation coefficient

Microvolume Protein Concentration Determination Using the NanoDrop Spectrophotometer

Microvolume samples are quantified by a UV/Vis spectrophotometer that uses surface tension to retain samples without the use of cuvettes or capillaries. The dynamic range of protein concentrations without dilutions and speed by which they can be measured are greatly increased with this method.

Launch Video

2 Comments Published: 11/04/2009

Philippe Desjardins
Joel B. Hansen
Michael Allen

Thermo Scientific NanoDrop Products

Basic Protocols, Issue 33, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

纳微量2000c 分光光度计- 微量体积蛋白浓度的测定

Launch Video

Published: 11/01/2009

Philippe Desjardins
Joel B. Hansen
Michael Allen

Thermo Scientific NanoDrop Products

Translation, Basic Protocols, Issue 33, NanoDrop, protein measurement, protein concentration, spectrophotometer, A280, UV/Vis, BCA, microvolume, microsample, proteomics

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.

Launch Video

Published: 10/01/2009

Eric Johansen¹
Birgit Schilling²
Michael Lerch¹
Richard K. Niles¹
Haichuan Liu¹
Bensheng Li²
Simon Allen¹
Steven C. Hall¹
H. Ewa Witkowska¹
Fred E. Regnier³
Bradford W. Gibson²
Susan J. Fisher¹
Penelope M. Drake¹

¹Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco

²Buck Institute for Age Research

³Department of Chemistry, Purdue University

Basic Protocols, Issue 32, Lectins, chromatography, glycopeptides, glycoproteins, biomarker discovery

Large Insert Environmental Genomic Library Production

Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.

Launch Video

1 Comment Published: 09/23/2009

Marcus Taupp
Sangwon Lee
Alyse Hawley
Jinshu Yang
Steven J. Hallam

Department of Microbiology and Immunology, University of British Columbia

Basic Protocols, Issue 31, environmental genomic, metagenomic, genomic DNA, large insert library, fosmid, phage packaging, automated colony picking

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Total: 40 articles.

Search results for 2/9/2010 1:06:48 PM

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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