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A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

Launch Video

Published: 04/19/2010

Derek L. Englert¹
Michael D. Manson²
Arul Jayaraman^{1, 3}

¹McFerrin Department of Chemical Engineering, Texas A&M University

²Department of Biology, Texas A&M University

³Department of Biomedical Engineering, Texas A&M University

Microbiology, Issue 38, chemotaxis, microfluidics, gradients

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.

Launch Video

Published: 01/26/2010

Adeola F. Adewola¹
Yong Wang¹
Tricia Harvat¹
David T. Eddington²
Dongyoung Lee¹
Jose Oberholzer^{1, 2}

¹Department of Surgery, University of Illinois, Chicago

²Department of Bioengineering, University of Illinois, Chicago

Cellular Biology, Issue 35, Microfluidics, Islet perifusion, glucose ramp, imaging, perifusion, beta cells, insulin secretion

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.

Launch Video

Published: 12/31/2009

William N. White¹
Palaniappan Sethu²

¹Department of Mechanical Engineering, University of Louisville

²Department of Bioengineering, University of Louisville

Cellular Biology, Issue 34, Microfluidics, Blood lysis, Cell Enrichment, Circulating Nucleated Cells, leukocytes, flow cytometry, FACS

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.

Launch Video

Published: 11/06/2009

Mais J. Jebrail¹
Vivienne N. Luk^{1, 2}
Steve C. C. Shih^{2, 3}
Ryan Fobel^{2, 3}
Alphonsus H. C. Ng^{2, 3}
Hao Yang¹
Sergio L. S. Freire¹
Aaron R. Wheeler^{1, 2, 3}

¹Department of Chemistry, University of Toronto

²Donnelly Centre for Cellular and Biomolecular Research

³Institute for Biomaterials and Biomedical Engineering, University of Toronto

Bioengineering, Issue 33, digital microfluidics, protein processing, protein extraction, protein precipitation, biochemical assays, reduction, alkylation, digestion, automation, feedback

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

In this article we describe how we obtain FRET traces from individual DNA molecules immobilized to a surface using an automated scanning confocal microscope.

Launch Video

2 Comments Published: 11/02/2009

Nicolas Di Fiori¹
Amit Meller^{1, 2}

¹Physics Department, Boston University

²Department of Biomedical Engineering, Boston University

Cellular Biology, Issue 33, single molecule FRET, DNA, surface immobilization, microfluidics, confocal microscope

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

We developed a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research. The platform is capable of conducting up to six independent experiments in parallel and was fabricated using a newly developed macro/micro hybrid fabrication method.

Launch Video

Published: 09/10/2009

Jaewon Park¹
Hisami Koito²
Jianrong Li²
Arum Han¹

¹Department of Electrical and Computer Engineering, Texas A & M University

²Department of Veterinary Integrative Biosciences, Texas A & M University

Biomedical Engineering, Issue 31, Neuron culture, neuron-glia interaction, microfluidics, cell culture microsystem

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

We present a novel and powerful integration of nanophotonics (QD-FRET) and microfluidics to investigate the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

Launch Video

Published: 08/26/2009

Yi-Ping Ho^{1, 2}
Hunter H. Chen^{2, 3}
Kam W. Leong²
Tza-Huei Wang^{3, 1}

¹Mechanical Engineering, Johns Hopkins University

²Biomedical Engineering, Duke University

³Biomedical Engineering, Johns Hopkins University

Biomedical Engineering, Issue 30, microfluidics, gene delivery, quantum dots, fluorescence resonance energy transfer, self-assembly, nanocomplexes

The Microfluidic Probe: Operation and Use for Localized Surface Processing

In this video we present the microfluidic probe¹ (MFP). We explain in detail how to assemble the MFP, mount it atop an inverted microscope, and align it relative to the substrate surface, and finally show how to use it to process a substrate surface immersed in a buffer solution.

Launch Video

Published: 06/04/2009

Cecile M. Perrault
Mohammad A. Qasaimeh
David Juncker

Department of Biomedical Engineering, McGill University

Bioengineering, Issue 28, microfluidics, integrated microfluidic system, bioMEMs

AC Electrokinetic Phenomena Generated by Microelectrode Structures

Manipulating fluids and suspended particles in the micro- and nano-scale is becoming more of a reality as enabling technologies, like AC electrokinetics, continue to develop. Here, we discuss the physics behind AC electrokinetics, how to fabricate these devices and how to interpret the experimental observations.

Launch Video

Published: 07/28/2008

Robert Hart¹
Jonghyun Oh²
Jorge Capurro²
Hongseok (Moses) Noh²

¹Biomedical Engineering, Science and Health Systems, Drexel University

²Mechanical Engineering and Mechanics, Drexel University

Bioengineering, Issue 17, AC Electrokinetics, AC Electroosmosis, Dielectrophoresis, Electrothermal Effect, Microelectrode, Microfluidics, Simulation, Microsphere, Microfabrication

Fabrication of the Thermoplastic Microfluidic Channels

Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.

Launch Video

5 Comments Published: 02/03/2008

Arpita Bhattacharyya
Dominika Kulinski
Catherine Klapperich

Department of Biomedical Engineering, Boston University

Cellular Biology, Issue 12, bioengineering, purification, microfluidics, DNA, RNA, solid phase, column

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Total: 24 articles.

Search results for 7/31/2010 4:09:32 PM

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

ISSN 1940-087X

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