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Trypsinizing and Subculturing Mammalian Cells
Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory, Inc.
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0:00 Title0 0:21 Subscription Lock21 0:37 Introduction37 0:56 Trypsinizig and Subculturing Mammalian cells from a Monolayer56 4:00 Passaging Cells in Suspension Culture240 5:36 Conclusion336
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As cells reach confluency, they must be subcultured or passaged. Failure to subculture confluent cells results in reduced mitotic index and eventually in cell death. The first step in subculturing is to detach cells from the surface of the primary culture vessel by trypsinization or mechanical means. The resultant cell suspension is then subdivided, or reseeded, into fresh cultures. Secondary cultures are checked for growth and fed periodically, and may be subsequently subcultured to produce tertiary cultures. The time between passaging of cells varies with the cell line and depends on the growth rate.
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Ricardo R, Phelan K (2008). Trypsinizing and Subculturing Mammalian Cells. JoVE. 16. http://www.jove.com/index/details.stp?id=755, doi: 10.3791/755
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Allowed tags: i, b, u, sup, sub 
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| | 09/11/2008 3:10:05 AM
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amit responded with a statement of type: Neutral
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how to prepare cell growth curve of cancer cell line |
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| | 09/11/2008 3:11:18 AM
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amit responded with a statement of type: Question
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how to prepare cell growth curve of cancer cell line |
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| | 09/18/2008 12:10:47 PM
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nav responded with a statement of type: Question
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can i know the passaging protocol for MDA MB 231 Breast cancer cells. |
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| | 09/24/2008 12:24:28 PM
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sam responded with a statement of type: Neutral
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sir can u tell me that how to transfer cell ( frozen into liq. nitrogen) into media and that will be the mother culture so in that also we have to do passaging , means we have to add HBSS and Trypsin and EDTA. and how to count cells.
thank you
waiting for your reply....
sam A Masih |
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| | 10/22/2008 7:45:39 PM
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kamal prajapati responded with a statement of type: Question
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Hi,
For subculturing suspension cells, what is the normal routine? is it better if changed everyday or only after the media contents turns trubid and yellowish colour?
Regards
Kamal |
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| | 07/28/2009 7:18:12 AM
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hemanta responded with a statement of type: Neutral
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Hello kamal bro..
paras sir le bhannu bhayeko ta tyahi ho kya re...
hemanta
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| | 04/02/2009 12:37:38 PM
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preethi_p2 responded with a statement of type: Neutral
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Could you elaborate on how to culture cancer cells? The precautions to be taken and when to subculture them.
Thank you |
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| | 06/22/2009 12:25:21 PM
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RF responded with a statement of type: Neutral
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I can see the video
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| | 06/23/2009 7:52:18 PM
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nikitab responded with a statement of type: Neutral
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We were having a technical issue when you posted your comment. Please let us know at support@jove.com if you are unable to view this video. |
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Mailer.URL: http://www.jove.com/index/details.stp?id=755 |
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