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Freezing, Thawing, and Packaging Cells for Transport
Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory Network, Inc

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0:00 TItle0

0:21 Subscription Lock21

0:44 Introduction44

1:04 Freezing Human Cells Grown in Monolayer Cultures64

3:06 Freezing Cells Grown In Suspension Culture186

5:42 Preparing Cells for Transport342

7:09 Conclusion429
Abstract
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.
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The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Cite this Article
Ricardo R, Phelan K (2008). Freezing, Thawing, and Packaging Cells for Transport. JoVE. 17. http://www.jove.com/index/details.stp?id=757, doi: 10.3791/757
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07/02/2008

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doi: 10.3791/757 

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Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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