Journal of Visualized Experiments

Sign In
New Account Forgot Password
Search
Home Browse For Authors Subscribe
< Previous Article
Next Article >
Freezing, Thawing, and Packaging Cells for Transport
Richard Ricardo, Katy Phelan
Molecular Pathology Laboratory Network, Inc

Sorry for the inconvenience.
This content requires the Adobe Flash Player 9 or higher.
Click Here to Get Flash

0:00 TItle0

0:21 Subscription Lock21

0:44 Introduction44

1:04 Freezing Human Cells Grown in Monolayer Cultures64

3:06 Freezing Cells Grown In Suspension Culture186

5:42 Preparing Cells for Transport342

7:09 Conclusion429

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.

 

You need a subscription to be able to access files

 

You must be subscribed to access this content.
Please click here to subscribe or get a FREE trial.

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

Ricardo R, Phelan K (2008). Freezing, Thawing, and Packaging Cells for Transport. JoVE. 17. http://www.jove.com/index/details.stp?id=757, doi: 10.3791/757
Post a Question / Comment / Request

Allowed tags: i, b, u, sup, sub

Please log into your account at the top of this page or

Create a New Account.

Sign your name and post:

Email:

Post Comment

Name:

OR

05/20/2009 9:59:30 AM
jabdo responded with a statement of type: Neutral

Thank

Reply to This
0
0

Mailer.URL:

http://www.jove.com/index/details.stp?id=757

 

Subscription Required

to view this article in its entirety

Free Trial
Subscribe
Recommend to Librarian

Info

07/02/2008

Publish Date

15350 

Views

 1 

Comments

doi: 10.3791/757 

Traffic Stats

Tracking

You must be logged in to track comments.

Share this article:

 Email this Article

My Stuff (Please log in to use)

Store a note

Make a Suggestion

Is there a technique you would like to see published?  Click the button above to make a suggestion.

 

 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

Quick links

 

* Welcome  

* Browse  

* Submit  

* About  

* Editorial Board  

* Press  

* Advertising

* Contact  

* Policies

* Article Index

* Sponsors

* Press Access

* Troubleshooting

 

 

* Twitter.com/JoveJournal

* FaceBook

Subscribe

You don't have to create an account to receive email notifications. 

Please enter your email:

Submit

Copyright (C) JoVE 2006-2008 All Rights Reserved.  Powered by TARGET.  JoVE Site Version 1.893

 

 

Feedback Form