Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

Jacky Dragons

Jacky lizards (A. muricatus) were held in climate-controlled captive facilities.

Enclosures were constructed of fabricated glass terrariums measuring 60 cm³. Enclosures were also filled with Sydney sand substrate, native foliage, and natural wooden perches. Enclosures were placed on top of movable trolleys that allowed for subjects to be placed in the middle of all viewing monitors.

Lizards were placed under a 14L:10D light cycle during simulated summer conditions, and were provided with both heat lamps (125W) and adequate ultraviolet (UV) lighting (300W) during the first half hour of daylight. Lizards were also provided with water ad libitum, fed crickets (Achetus domesticus) dusted with vitamins during non-experimental sessions, and routinely wormed for parasites using diluted doses of Ivermectin.

Equipment Used for Playback

Three video playback rigs are used for stimulus presentation (see Figure 1). The central rig consists of an iMac (Apple Computer Inc.) containing dot stimuli. The two additional rigs consist of an iMac with Final Cut Pro HD for stimulus presentation that contained playback stimuli (i.e., invertebrates).

The digital signal is sent to an advanced digital video converter (Canopus©gt ADVC110, Melbourne, Victoria Australia) for digital-to-analog conversion for video playback and displayed on a Sony Trintron (Sony Corporation, Shinagawa, Tokyo Japan) monitor (Series Nos. PVM-14M2A/PVM-14L2/PVM-14N5A). A Canon (Model No. MV650i) digital camcorder with 22X optical zoom (Cannon Inc., Japan) is mounted directly above the testing area and records responses to stimuli directly to a Sony (Sony Corporation, Shinagawa, Tokyo Japan) video cassette recorder (Serial No. SLV-EZ717AS).

Recorded images are sent to a colour viewfinder (NEC, Serial No. 1892, Kanagawa, Japan). At 7.5 seconds from the start of the dots display, an electronically generated sound marker is sent to a Canopus® ADVC110 and records onto VHS. Video playback is adjusted for PAL-DV standard (5:1 compression; horizontal resolution 575 lines; 25 frames s-1).

Random-dot Kinematograms (RDKs)

RDKs can be developed using VPixx V1.79 (VPixx V1.79, VPixx Technologies Inc. 2002).

Design stimuli with a 30 x 20 degree field, fill with 200 dots, with each dot measuring 5 x 5 pixels.

Build dot stimuli with eight variations in speed combined with eight levels of coherence and two directions, generating 128 different stimuli.

Speed parameters were designed in degrees per second (°/s). Speed variations were 0.5, 2.5, 5, 10, 20, 40, 80, and 160°/s. Coherence percentages were 0, 2.5, 5, 10, 20, 40, 80, and 100% in either a left or right directional motion.

Export each sequence as a Motion JPEG-B file. However, you can design the RDKs which an array of features.

Each sequence is a total 15 s in duration. The sequence begins with 2.5 s of 0% coherence matched with the trial speed variable. This period initiates the current trial. This is followed by 5 s of the variation in speed and coherence combination. The sequence was completed by 7.5 s of 100% in the appropriate direction.

Import all Motion JPEG-B sequences and edit in Apple Final Cut Pro HD (Apple Computers Inc.). Add a sound marker at each interval. This marker is only audible to VHS, and is used as a cue when scoring behaviour when the changes in stimulus presentation occur. Exported each of the 128 sequences as Final Cut Pro movies.

Primary reinforcers - Mealworms

Place subjects on a randomised reinforcement schedule of five trials per block trial (16 trials) during the experiment. Primary reinforcers are mealworms (Tenebrio molitor).

Secondary reinforcers - Animated Invertebrates

Secondary reinforcers are to be paired with the primary reinforcer. Created biologically meaningful reinforcers, such as three types of computer-animated invertebrates: 1) cricket, 2) mite, and 3) spider.

Create animations using Lightwave v8.3 (NewTek Inc., San Antonio, TX, USA) for 15 s in length, with the initial 7.5 s of still background, and followed by 7.5 s of the invertebrate on the appropriate screen.

Photograph a background of natural vegetation to import as a JPEG in eah clip. Export each sequence into individual JPEG image sequences (375 frames), and compress into a movie file using Final Cut Pro HD for video playback. Design 50 unique clips to prevent habituation to the conditioning paradigm.

General Procedure

Place subjects between the viewing monitors.

Randomize presentation of RDK stimuli for each subject.

Show stimuli in blocks of 16 trials over eight days. Each trial is 15 s with intertrial intervals varying from 30-60 s. Segment each trial interval into 2.5 s of 0% coherence reflective of testing speed, 5 s of testing speed and coherence, and followed by 7.5 s of 100% in appropriate direction matched with speed.

During this 5 s critical-period, subjects are required to respond. Record dependent measures as orientation towards a left or right monitor and latency to respond.

Use only five random trials with a primary reinforcer and reinforce each trial with an animated invertebrate. At the completion of each block trial, remove subject enclosures from the testing area, and introduce another enclosure.

Testing the sensory capacity in lizard species has been difficult, and has largely been both a tedious and arduous process. These difficulties stem from finding the correct reinforcer or establishing a functional protocol. Here, I established a successful protocol in which to test motion perception in lizards. This procedure may also be applicable in testing other visual characteristics as well as other sensory mechanisms. In addition, this procedure may be applicable to other species. The usefulness of this protocol not only allows us to understand natural behaviour, but to use lizard behaviour as a model for other scientific endeavours.