Journal of Visualized Experiments

Sign In
New Account Forgot Password
Search
Home Browse For Authors Subscribe
< Previous Article
Next Article >
Experion RNA Assay - Bio-Rad - ADVERTISEMENT

Sorry for the inconvenience.
This content requires the Adobe Flash Player 9 or higher.
Click Here to Get Flash

The Experion™ RNA Run video provides a detailed explanation of how RNA run data are generated using the Experion automated electrophoresis system and highlights the salient features that help evaluate the RNA analysis data. The video offers a brief glimpse into the technology of electropherogram generation and goes through the RNA run with the aid of screenshots generated using the Experion software. It takes the viewer through evaluation of both total RNA assays and mRNA assays, how many RNA peaks to expect, how they should look, where they should lie, how they differ in the two assays, and what considerations need to be made for assuring a successful run. In addition to aiding in the interpretation of the electropherogram data, the video also explains how the RQI (RNA quality indicator) value that the Experion software generates can be used to assess RNA integrity and how to decipher a poor quality of RNA as reflected in electropherograms. It displays virtual gel images for both total RNA and mRNA assays and describes the features that should be visible in a virtual gel following a successful RNA run.

NA (2009). Experion RNA Assay - Bio-Rad - ADVERTISEMENT. JoVE. http://www.jove.com/index/details.stp?id=1547, doi: 10.3791/1547
Post a Question / Comment / Request

Allowed tags: i, b, u, sup, sub

Please log into your account at the top of this page or

Create a New Account.

Sign your name and post:

Email:

Post Comment

Name:

OR

11/04/2009 10:47:48 AM
pook responded with a statement of type: Question
i used to use this product and the result found that RNa ladder is not enough
pls help me
Reply to This
0
0
11/15/2009 1:14:05 PM
Hackmann responded with a statement of type: Neutral
is it possible to detect rRNA processing defects withinthe electropherogramm?
Reply to This
0
0
01/28/2010 5:39:58 AM
SARANG responded with a statement of type: Neutral
how many no. of dNTPS are found in 1 micro litre during pcr????????????? plz reply on sarangbiotech@gmail.com putting subject as dNTPSSSSS
Reply to This
0
0

Mailer.URL:

http://www.jove.com/index/details.stp?id=1547

 

Advertisement

Sponsorship Disclaimer
Publication Criteria

Info

07/12/2009

Publish Date

6132 

Views

 3 

Comments

doi: 10.3791/1547 

Tracking

You must be logged in to track comments.

Share this article:

 Email this Article

My Stuff (Please log in to use)

Store a note

Make a Suggestion

Is there a technique you would like to see published?  Click the button above to make a suggestion.

 

 

About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

Quick links

 

* Welcome  

* Browse  

* Submit  

* About  

* Editorial Board  

* Press  

* Advertising

* Contact  

* Policies

* Article Index

* Sponsors

* Press Access

* Troubleshooting

* Minerva

 

 

* Twitter.com/JoveJournal

* FaceBook

Subscribe

You don't have to create an account to receive email notifications. 

Please enter your email:

Submit

Copyright (C) JoVE 2006-2008 All Rights Reserved.  Powered by TARGET.  JoVE Site Version 1.893