Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
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07/25/2010
Pradeepa Jayachandran1, Elim Hong2, Rachel Brewster2
1Center for Neuroscience, Children’s National Medical Center, 2Department of Biological Sciences, University of Maryland Baltimore County
Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.
07/24/2010
Christian Georg Habeck
Department of Neurology, Columbia University
The current article describes the basics of multivariate analysis and contrasts it to the more commonly used voxel-wise univariate analysis. Both types of analysis are applied to a clinical-neuroscience data set. Supplementary split-half simulations show better replication of the multivariate results in independent data sets.
07/22/2010
Esther Y. Maier1, 2, Sean T. Ma3, Allison Ahrens2, 4, Timothy J. Schallert2, 4, 5, Christine L. Duvauchelle1, 2, 4
1College of Pharmacy, Division of Pharmacology and Toxicology, University of Texas at Austin, 2The Waggoner Center of Addiction and Alcohol Research, University of Texas at Austin, 3Department of Psychology, University of Michigan, 4Institute for Neuroscience, University of Texas at Austin, 5Department of Psychology, University of Texas at Austin
Drug self-administration and ultrasonic vocalizations (USV) are used as behavioral assessments in animal research, but rarely in combination. The purpose of this article is to describe the advantages of recording USVs during drug self-administration procedures to assess affective responses to drug experience.
07/21/2010
Kelly A. Tennant1, Aaron L. Asay2, Rachel P. Allred3, Angela R. Ozburn4, Jeffrey A. Kleim5, Theresa A. Jones1, 2
1Institute for Neuroscience, University of Texas at Austin, 2Department of Psychology, University of Texas at Austin, 3Department of Neurology, University of Florida, 4Department of Psychiatry, University of Texas Southwestern Medical Center at Dallas, 5Department of Neuroscience, McKnight Brain Institute, University of Florida
The Vermicelli and Capellini Handling Tests of forepaw dexterity take advantage of the natural inclination of rodents to manipulate food items using skillful forepaw and digit movements. Animals are videotaped while handling short strands of uncooked dry pasta. Slow motion video playback allows for the quantification of forepaw adjustments.
07/20/2010
Marie-Ève Tremblay1, Mustapha Riad2, Ania Majewska1
1Department of Neurobiology and Anatomy, University of Rochester Medical Center, 2Douglas Mental Health University Institute
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
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