Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
|
|
07/12/2010
Robert Sucher1,*, Rupert Oberhuber1,*, Christian Margreiter1, Guido Rumberg1, Rishi Jindal2, WP Andrew Lee2, Raimund Margreiter1, Johann Pratschke1, Stefan Schneeberger1, Gerald Brandacher1
1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center
* These authors contributed equally
Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.
07/10/2010
Sreemanti Basu, Hope M. Campbell, Bonnie N. Dittel, Avijit Ray
Blood Research Institute, BloodCenter of Wisconsin
For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.
07/08/2010
Christina Farr1, Stuart Berger1, 2
1Immunology, University of Toronto, 2Arthritis and Immune Disorder Research Centre, University Health Network
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
06/01/2010
Craig T. Lefort, Minsoo Kim
Center for Vaccine Biology and Immunology, University of Rochester
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
04/22/2010
Hong He1, Amy N. Courtney1, Eric Wieder2, K. Jagannadha Sastry1
1Department of Immunology, The University of Texas M.D. Anderson Cancer Center, 2Department of Medicine, University of Miami
We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.
Read More Articles >>
Get articles delivered to you via RSS: |
|
Make a SuggestionIs there a technique you would like to see published? Click the button above to make a suggestion. |
|
|
|
 |